Ceramide monoclonal antibody (MID 15B4) | Enzo Life Sciences ALX-804-196-T050 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Ceramide monoclonal antibody (MID 15B4)

catalog :

ALX-804-196-T050

quantity :

50 tests

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MID 15B4

reactivity :

human, mouse, rat, dogs

application :

ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section, blocking or activating experiments

more info or order :

Enzo Life Sciences product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3a	Jeong H, Lim K, Goldenring J, Nam K. Rab25 Deficiency Perturbs Epidermal Differentiation and Skin Barrier Function in Mice. Biomol Ther (Seoul). 2019;27:553-561 pubmed publisher
immunocytochemistry; human; loading ...; fig 1f	Oleinik N, Kim J, Roth B, Selvam S, Gooz M, Johnson R, et al. Mitochondrial protein import is regulated by p17/PERMIT to mediate lipid metabolism and cellular stress. Sci Adv. 2019;5:eaax1978 pubmed publisher
immunocytochemistry; human; loading ...; fig 7a	Xiong X, Lee C, Li W, Yu J, Zhu L, Kim Y, et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open. 2019;: pubmed publisher
immunocytochemistry; human; 1:50; loading ...; fig 3m	Tawk C, Nigro G, Rodrigues Lopes I, Aguilar C, Lisowski C, Mano M, et al. Stress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens. EMBO J. 2018;37: pubmed publisher
blocking or activating experiments; mouse; loading ...; fig 6b	Shiba E, Izawa K, Kaitani A, Isobe M, Maehara A, Uchida K, et al. Ceramide-CD300f Binding Inhibits Lipopolysaccharide-induced Skin Inflammation. J Biol Chem. 2017;292:2924-2932 pubmed publisher
immunocytochemistry; human; 1:30; fig 4a	Boppana N, Stochaj U, Kodiha M, Bielawska A, Bielawski J, Pierce J, et al. Enhanced killing of SCC17B human head and neck squamous cell carcinoma cells after photodynamic therapy plus fenretinide via the de novo sphingolipid biosynthesis pathway and apoptosis. Int J Oncol. 2015;46:2003-10 pubmed publisher
immunocytochemistry; rat; 1:250	Jaishy B, Zhang Q, Chung H, Riehle C, Soto J, Jenkins S, et al. Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity. J Lipid Res. 2015;56:546-61 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:10	Yang D, Stavrides P, Saito M, Kumar A, Rodriguez Navarro J, Pawlik M, et al. Defective macroautophagic turnover of brain lipids in the TgCRND8 Alzheimer mouse model: prevention by correcting lysosomal proteolytic deficits. Brain. 2014;137:3300-18 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:10	Kim H, Al Mahrouki A, Gorjizadeh A, Sadeghi Naini A, Karshafian R, Czarnota G. Quantitative ultrasound characterization of tumor cell death: ultrasound-stimulated microbubbles for radiation enhancement. PLoS ONE. 2014;9:e102343 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:10	Kim H, Al Mahrouki A, Gorjizadeh A, Karshafian R, Czarnota G. Effects of biophysical parameters in enhancing radiation responses of prostate tumors with ultrasound-stimulated microbubbles. Ultrasound Med Biol. 2013;39:1376-87 pubmed publisher
	Gencer S, Oleinik N, Kim J, Panneer Selvam S, De Palma R, Dany M, et al. TGF-β receptor I/II trafficking and signaling at primary cilia are inhibited by ceramide to attenuate cell migration and tumor metastasis. Sci Signal. 2017;10: pubmed publisher
	Al Mamun Bhuyan A, Signoretto E, Bissinger R, Lang F. Enhanced Eryptosis Following Exposure to Dolutegravir. Cell Physiol Biochem. 2016;39:639-50 pubmed publisher
	Peter T, Bissinger R, Signoretto E, Mack A, Lang F. Micafungin-Induced Suicidal Erythrocyte Death. Cell Physiol Biochem. 2016;39:584-95 pubmed publisher
	Peter T, Bissinger R, Liu G, Lang F. Anidulafungin-Induced Suicidal Erythrocyte Death. Cell Physiol Biochem. 2016;38:2272-84 pubmed publisher
	Al Mahrouki A, Wong E, Czarnota G. Ultrasound-stimulated microbubble enhancement of radiation treatments: endothelial cell function and mechanism. Oncoscience. 2015;2:944-57 pubmed
	Qadri S, Donkor D, Bhakta V, Eltringham Smith L, Dwivedi D, Moore J, et al. Phosphatidylserine externalization and procoagulant activation of erythrocytes induced by Pseudomonas aeruginosa virulence factor pyocyanin. J Cell Mol Med. 2016;20:710-20 pubmed publisher
	Bouguerra G, Bissinger R, Abbes S, Lang F. Stimulation of Eryptosis by Narasin. Cell Physiol Biochem. 2015;37:1807-16 pubmed publisher
	Bouguerra G, Aljanadi O, Bissinger R, Abbes S, Lang F. Embelin-Induced Phosphatidylserine Translocation in the Erythrocyte Cell Membrane. Cell Physiol Biochem. 2015;37:1629-40 pubmed publisher
	Bissinger R, Malik A, Bouguerra G, Zhou Y, Singh Y, AbbÃ¨s S, et al. Triggering of Suicidal Erythrocyte Death by the Antibiotic Ionophore Nigericin. Basic Clin Pharmacol Toxicol. 2016;118:381-9 pubmed publisher
	Lupescu A, Bissinger R, Goebel T, Salker M, Alzoubi K, Liu G, et al. Enhanced suicidal erythrocyte death contributing to anemia in the elderly. Cell Physiol Biochem. 2015;36:773-83 pubmed publisher
	Malik A, Bissinger R, Liu G, Liu G, Lang F. Enhanced eryptosis following gramicidin exposure. Toxins (Basel). 2015;7:1396-410 pubmed publisher
	Boppana N, Stochaj U, Kodiha M, Bielawska A, Bielawski J, Pierce J, et al. C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy. J Photochem Photobiol B. 2015;143:163-8 pubmed publisher
	CalabrÃ² S, Alzoubi K, Bissinger R, Faggio C, Lang F. Stimulation of suicidal erythrocyte death by ellipticine. Basic Clin Pharmacol Toxicol. 2015;116:485-92 pubmed publisher
	Gills J, Zhang C, Abu Asab M, Castillo S, Marceau C, LoPiccolo J, et al. Ceramide mediates nanovesicle shedding and cell death in response to phosphatidylinositol ether lipid analogs and perifosine. Cell Death Dis. 2012;3:e340 pubmed publisher
	Korbelik M, Zhang W, Separovic D. Monitoring ceramide and sphingosine-1-phosphate levels in cancer cells and macrophages from tumours treated by photodynamic therapy. Photochem Photobiol Sci. 2012;11:779-84 pubmed publisher
	Jilani K, Abed M, Zelenak C, Lang E, Qadri S, Lang F. Triggering of erythrocyte cell membrane scrambling by ursolic acid. J Nat Prod. 2011;74:2181-6 pubmed publisher
	Kim S, Steelman A, Zhang Y, Kinney H, Li J. Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury. Brain Pathol. 2012;22:41-57 pubmed publisher
	Zhang X, Wu X, Li J, Sun Y, Gao P, Zhang C, et al. MDR1 (multidrug resistence 1) can regulate GCS (glucosylceramide synthase) in breast cancer cells. J Surg Oncol. 2011;104:466-71 pubmed publisher

product information

Product ID :

ALX-804-196

Catalog Number :

ALX-804-196-T050

Product Name :

Ceramide monoclonal antibody (MID 15B4)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 tests

list of Pubmed id :

26584281, 26021265, 25635908, 25529920, 25051356, 22764099, 22354109, 21923134, 21615590, 21538359

Clone :

MID 15B4

Isotype :

IgM

SPECIES REACTIVITY :

Species independent

Immunogen :

Ceramide (sphingosine-[trans-D-erythro-2-amino-4-octadecene-1,3-diol]) conjugated to BSA.

Specificity :

Recognizes C16- and C24-ceramide, dihydroceramide, sphingomyelin and phosphatidylcholine in highly artificial lipid overlay test systems. Under more physiological in vitro and in vivo conditions highly specific for ceramide and does not cross-react with sphingomyelin, cholesterol or other phospholipids.

Buffer :

Liquid. In PBS, pH 7.2, containing 0.5M sodium chloride, 0.1% BSA and 0.09% sodium azide.

Application Summary :

ELISA, FC, ICC, IHC (PS)

Storage :

+4°C

more info or order :

Enzo Life Sciences product webpage