Laminin-2 (α-2-chain) monoclonal antibody (4H8-2) | Enzo Life Sciences ALX-804-190-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Laminin-2 (α-2-chain) monoclonal antibody (4H8-2)

catalog :

ALX-804-190-C100

quantity :

100 µg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

4H8-2

reactivity :

human, mouse

application :

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Enzo Life Sciences product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; loading ...; fig 2k	Loo T, Ye X, Chai R, Ito M, Bonne G, Ferguson Smith A, et al. The mammalian LINC complex component SUN1 regulates muscle regeneration by modulating drosha activity. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 4b	Takemoto Y, Inaba S, Zhang L, Tsujikawa K, Uezumi A, Fukada S. Implication of basal lamina dependency in survival of Nrf2-null muscle stem cells via an antioxidative-independent mechanism. J Cell Physiol. 2019;234:1689-1698 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:400; fig 4	Lee S, Won J, Yang J, Lee J, Kim S, Lee E, et al. AKAP6 inhibition impairs myoblast differentiation and muscle regeneration: Positive loop between AKAP6 and myogenin. Sci Rep. 2015;5:16523 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:250; loading ...; fig s2b	Di Rocco A, Uchibe K, Larmour C, Berger R, Liu M, Barton E, et al. Selective Retinoic Acid Receptor Î³ Agonists Promote Repair of Injured Skeletal Muscle in Mouse. Am J Pathol. 2015;185:2495-504 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 4d	Lee S, Yang J, Kim S, Jeong H, Lee J, Kim W, et al. MicroRNA-26a induced by hypoxia targets HDAC6 in myogenic differentiation of embryonic stem cells. Nucleic Acids Res. 2015;43:2057-73 pubmed publisher
immunohistochemistry - frozen section; mouse; fig s1	WirsdÃ¶rfer F, Bangen J, Pastille E, Hansen W, FlohÃ© S. Breaking the co-operation between bystander T-cells and natural killer cells prevents the development of immunosuppression after traumatic skeletal muscle injury in mice. Clin Sci (Lond). 2015;128:825-38 pubmed publisher
immunohistochemistry - frozen section; human	von Renesse A, Petkova M, Lützkendorf S, Heinemeyer J, Gill E, Hübner C, et al. POMK mutation in a family with congenital muscular dystrophy with merosin deficiency, hypomyelination, mild hearing deficit and intellectual disability. J Med Genet. 2014;51:275-82 pubmed publisher
immunohistochemistry - frozen section; human; 1:100	Cirak S, Foley A, Herrmann R, Willer T, Yau S, Stevens E, et al. ISPD gene mutations are a common cause of congenital and limb-girdle muscular dystrophies. Brain. 2013;136:269-81 pubmed publisher
	Jarad G, Pippin J, Shankland S, Kreidberg J, Miner J. Dystroglycan does not contribute significantly to kidney development or function, in health or after injury. Am J Physiol Renal Physiol. 2011;300:F811-20 pubmed publisher
	Mizuno Y, Chang H, Umeda K, Niwa A, Iwasa T, Awaya T, et al. Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells. FASEB J. 2010;24:2245-53 pubmed publisher
	Goldberg S, Adair Kirk T, Senior R, Miner J. Maintenance of glomerular filtration barrier integrity requires laminin alpha5. J Am Soc Nephrol. 2010;21:579-86 pubmed publisher
	Mori J, Ishihara Y, Matsuo K, Nakajima H, Terada N, Kosaka K, et al. Hematopoietic contribution to skeletal muscle regeneration in acid alpha-glucosidase knockout mice. J Histochem Cytochem. 2008;56:811-7 pubmed publisher
	Patton B, Wang B, Tarumi Y, Seburn K, Burgess R. A single point mutation in the LN domain of LAMA2 causes muscular dystrophy and peripheral amyelination. J Cell Sci. 2008;121:1593-604 pubmed publisher
	Nguyen N, Pulkkinen L, Schlueter J, Meneguzzi G, Uitto J, Senior R. Lung development in laminin gamma2 deficiency: abnormal tracheal hemidesmosomes with normal branching morphogenesis and epithelial differentiation. Respir Res. 2006;7:28 pubmed
	Miner J, Li C, Patton B. Laminins alpha2 and alpha4 in pancreatic acinar basement membranes are required for basal receptor localization. J Histochem Cytochem. 2004;52:153-6 pubmed
	Yuasa K, Fukumoto S, Kamasaki Y, Yamada A, Fukumoto E, Kanaoka K, et al. Laminin alpha2 is essential for odontoblast differentiation regulating dentin sialoprotein expression. J Biol Chem. 2004;279:10286-92 pubmed
	Montanaro F, Liadaki K, Volinski J, Flint A, Kunkel L. Skeletal muscle engraftment potential of adult mouse skin side population cells. Proc Natl Acad Sci U S A. 2003;100:9336-41 pubmed

product information

Product ID :

ALX-804-190

Catalog Number :

ALX-804-190-C100

Product Name :

Laminin-2 (α-2-chain) monoclonal antibody (4H8-2)

Host species :

Rat

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

25662604, 21209007, 20181939, 20150535, 18505932, 18430779, 16483354, 14729866, 14681233, 12886022

Clone :

4H8-2

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse

Background :

The laminins are a family of glycoproteins that provide an integral part of the structural scaffolding of basement membranes in almost every tissue. Each laminin is a heterotrimer assembled from α, β and γ chain subunits, secreted and incorporated into cell-associated extracellular matrices. The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycans, and other receptors. Through these interactions, laminins critically contribute to cell differentiation, cell shape and movement, maintenance of tissue phenotypes and promotion of tissue survival.

ALTnames :

Merosin, LAMA2

Immunogen :

Mouse heart laminin-2 (merosin).

Specificity :

Recognizes an N-terminal portion of the α2 chain that is deleted in congenital muscular dystrophies.

Buffer :

Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.

Uses :

Not recommended for Western blot.

Application Summary :

ELISA, IHC, IP

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage