VASP monoclonal antibody (IE273) | Enzo Life Sciences ALX-804-177-C050 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

VASP monoclonal antibody (IE273)

catalog :

ALX-804-177-C050

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IE273

The same clone is also sold as:

Immunoglobe: 0016-blk, 0016-05
Abnova: MAB16120

reactivity :

human, bovine

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; fig s3	Latosinska A, Makridakis M, Frantzi M, Borràs D, Janssen B, Mullen W, et al. Integrative analysis of extracellular and intracellular bladder cancer cell line proteome with transcriptome: improving coverage and validity of -omics findings. Sci Rep. 2016;6:25619 pubmed publisher
immunocytochemistry; human; 1:50-1:100; loading ...; fig 4a	Tojkander S, Gateva G, Husain A, Krishnan R, Lappalainen P. Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly. elife. 2015;4:e06126 pubmed publisher
	Mülsch A, Oelze M, Kloss S, Mollnau H, Töpfer A, Smolenski A, et al. Effects of in vivo nitroglycerin treatment on activity and expression of the guanylyl cyclase and cGMP-dependent protein kinase and their downstream target vasodilator-stimulated phosphoprotein in aorta. Circulation. 2001;103:2188-94 pubmed
	Niebuhr K, Ebel F, Frank R, Reinhard M, Domann E, Carl U, et al. A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. EMBO J. 1997;16:5433-44 pubmed

product information

Product ID :

ALX-804-177

Catalog Number :

ALX-804-177-C050

Product Name :

VASP monoclonal antibody (IE273)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

11331261, 9312002, 9312002

Clone :

IE273

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Rabbit, Porcine, Bovine

Background :

VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:. the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a proline-rich motif, a more divergent proline-rich central domain (which is responsible for profilin binding), and a conserved C-terminal EVH2 domain.Particularly high VASP levels are present in platelets, although VASP is expressed in a wide variety of cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.

ALTnames :

Vasodilator stimulated phosphoprotein

Immunogen :

Purified human VASP (vasodilator stimulated phosphoprotein).

Specificity :

Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of VASP.

Buffer :

Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.

Application Summary :

ICC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage