PDI monoclonal antibody (RL90) | Enzo Life Sciences ALX-804-012-R100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

PDI monoclonal antibody (RL90)

catalog :

ALX-804-012-R100

quantity :

100 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RL90

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunocytochemistry knockout validation

more info or order :

Enzo Life Sciences product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; human; loading ...; fig 5b	Baumann S, Hennet T. Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase. J Biol Chem. 2016;291:18514-24 pubmed publisher
	Venkatagopalan P, Daskalova S, Lopez L, Dolezal K, Hogue B. Coronavirus envelope (E) protein remains at the site of assembly. Virology. 2015;478:75-85 pubmed publisher
	Lahav J, Jurk K, Hess O, Barnes M, Farndale R, Luboshitz J, et al. Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange. Blood. 2002;100:2472-8 pubmed
	Mandel R, Ryser H, Ghani F, Wu M, Peak D. Inhibition of a reductive function of the plasma membrane by bacitracin and antibodies against protein disulfide-isomerase. Proc Natl Acad Sci U S A. 1993;90:4112-6 pubmed
	Ryser H, Levy E, Mandel R, DiSciullo G. Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction. Proc Natl Acad Sci U S A. 1994;91:4559-63 pubmed
	Kaetzel C, Rao C, Lamm M. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J. 1987;241:39-47 pubmed

product information

Product ID :

ALX-804-012

Catalog Number :

ALX-804-012-R100

Product Name :

PDI monoclonal antibody (RL90)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µl

list of Pubmed id :

25726972, 12239158, 8387210, 8183947, 3566712

Clone :

RL90

Isotype :

IgG2a

SPECIES REACTIVITY :

Human, Mouse, Rat, Porcine, Hamster

Background :

The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

ALTnames :

Protein disulfide-isomerase

Immunogen :

Purified rat PDI (protein disulfide-isomerase).

Buffer :

Liquid. Purified antibody containing 1mg/ml BSA and 0.05% sodium azide.

Uses :

Detects a band of ~59kDa (rat liver extract) or ~61kDa (human liver extract) by Western blot.

Application Summary :

FC, ICC, IHC (FS), IHC (PS), IP, WB, FUNC

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage