DMPO monoclonal antibody (1) | Enzo Life Sciences ALX-803-340-C100 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

DMPO monoclonal antibody (1)

catalog :

ALX-803-340-C100

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

citations: 2

Reference
Takamiya M, Xu F, Suhonen H, Gourain V, Yang L, Ho N, et al. Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development. Sci Rep. 2016;6:25046 pubmed publisher
Chatterjee S, Ehrenshaft M, Bhattacharjee S, Deterding L, Bonini M, Corbett J, et al. Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic Biol Med. 2009;46:454-61 pubmed publisher

product information

Product ID :

ALX-803-340

Catalog Number :

ALX-803-340-C100

Product Name :

DMPO monoclonal antibody (1)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

19049863

Clone :

Isotype :

IgG

Product Description :

Oxidative stress resulting in the formation of biological radicals has been implicated in a number of human diseases, such as cancer and aging. There is, however, a paucity of reliable methods for in vivo or ex vivo detection of radical formation. Until now the only general technique that allowed for the detection of these highly reactive species was electron spin resonance (ESR) using spin traps. One of the most popular of these spin traps is 5,5-dimethyl-1-pyrroline N-oxide (DMPO). In the ESR method, radicals are trapped by DMPO, and the DMPO spin adduct signal is measured quantitatively by an ESR spectrometer. The DMPO, mAb (1) (Prod. No. ALX-803-340) specifically reacts with DMPO-protein and DMPO-DNA adducts and therefore highly simplifies the detection of radical formation compared to the ESR method.

SPECIES REACTIVITY :

Species independent

ALTnames :

5,5-dimethyl-1-pyrroline-N-oxide

Immunogen :

DMPO-octanoic acid conjugated to ovalbumin.

Specificity :

Recognizes DMPO (Prod. No. ALX-430-090), DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts.

Buffer :

Liquid. In PBS, pH 7.4, containing 10% glycerol and 0.02% sodium azide.

Application Summary :

ELISA, IP, WB

Storage :

+4°C