c-IAP1 monoclonal antibody (1E1-1-10) | Enzo Life Sciences ALX-803-335-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

c-IAP1 monoclonal antibody (1E1-1-10)

catalog :

ALX-803-335-C100

quantity :

100 µg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

1E1-1-10

reactivity :

human, mouse

application :

western blot

more info or order :

Enzo Life Sciences product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 4a, s3c western blot; human; 1:500; loading ...; fig s3c	Giampazolias E, Zunino B, Dhayade S, Bock F, Cloix C, Cao K, et al. Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency. Nat Cell Biol. 2017;19:1116-1129 pubmed publisher
western blot; mouse; loading ...; fig s5a	Newton K, Wickliffe K, Maltzman A, Dugger D, Strasser A, Pham V, et al. RIPK1 inhibits ZBP1-driven necroptosis during development. Nature. 2016;540:129-133 pubmed publisher
western blot; mouse; fig s10	Takahashi N, Vereecke L, Bertrand M, Duprez L, Berger S, Divert T, et al. RIPK1 ensures intestinal homeostasis by protecting the epithelium against apoptosis. Nature. 2014;513:95-9 pubmed publisher
western blot; mouse; fig 3	Dannappel M, Vlantis K, Kumari S, Polykratis A, Kim C, Wachsmuth L, et al. RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis. Nature. 2014;513:90-4 pubmed publisher
western blot; human; fig s1	Takeda A, Oberoi Khanuja T, Glatz G, Schulenburg K, Scholz R, Carpy A, et al. Ubiquitin-dependent regulation of MEKK2/3-MEK5-ERK5 signaling module by XIAP and cIAP1. EMBO J. 2014;33:1784-801 pubmed publisher
western blot; human; 1:1000	Annibaldi A, Heulot M, Martinou J, Widmann C. TAT-RasGAP317-326-mediated tumor cell death sensitization can occur independently of Bax and Bak. Apoptosis. 2014;19:719-33 pubmed publisher
western blot; mouse	Kenneth N, Younger J, Hughes E, Marcotte D, Barker P, Saunders T, et al. An inactivating caspase 11 passenger mutation originating from the 129 murine strain in mice targeted for c-IAP1. Biochem J. 2012;443:355-9 pubmed publisher
	Kreckel J, Anany M, Siegmund D, Wajant H. TRAF2 Controls Death Receptor-Induced Caspase-8 Processing and Facilitates Proinflammatory Signaling. Front Immunol. 2019;10:2024 pubmed publisher
	Rizk J, Kaplinsky J, Agerholm R, Kadekar D, Ivars F, Agace W, et al. SMAC mimetics promote NIK-dependent inhibition of CD4+ TH17 cell differentiation. Sci Signal. 2019;12: pubmed publisher
	Ohoka N, Okuhira K, Ito M, Nagai K, Shibata N, Hattori T, et al. In Vivo Knockdown of Pathogenic Proteins via Specific and Nongenetic Inhibitor of Apoptosis Protein (IAP)-dependent Protein Erasers (SNIPERs). J Biol Chem. 2017;292:4556-4570 pubmed publisher
	Siegmund D, Kums J, Ehrenschwender M, Wajant H. Activation of TNFR2 sensitizes macrophages for TNFR1-mediated necroptosis. Cell Death Dis. 2016;7:e2375 pubmed publisher
	de Almagro M, Goncharov T, Newton K, Vucic D. Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination. Cell Death Dis. 2015;6:e1800 pubmed publisher
	Lawlor K, Khan N, Mildenhall A, Gerlic M, Croker B, D Cruz A, et al. RIPK3 promotes cell death and NLRP3 inflammasome activation in the absence of MLKL. Nat Commun. 2015;6:6282 pubmed publisher
	Kocab A, Veloso A, Paulsen M, Ljungman M, Duckett C. Effects of physiological and synthetic IAP antagonism on c-IAP-dependent signaling. Oncogene. 2015;34:5472-81 pubmed publisher
	Darding M, Feltham R, Tenev T, Bianchi K, Benetatos C, Silke J, et al. Molecular determinants of Smac mimetic induced degradation of cIAP1 and cIAP2. Cell Death Differ. 2011;18:1376-86 pubmed publisher
	Feltham R, Moulin M, Vince J, Mace P, Wong W, Anderton H, et al. Tumor necrosis factor (TNF) signaling, but not TWEAK (TNF-like weak inducer of apoptosis)-triggered cIAP1 (cellular inhibitor of apoptosis protein 1) degradation, requires cIAP1 RING dimerization and E2 binding. J Biol Chem. 2010;285:17525-36 pubmed publisher
	Lluis J, Nachbur U, Cook W, Gentle I, Moujalled D, Moulin M, et al. TAK1 is required for survival of mouse fibroblasts treated with TRAIL, and does so by NF-kappaB dependent induction of cFLIPL. PLoS ONE. 2010;5:e8620 pubmed publisher
	Geserick P, Hupe M, Moulin M, Wong W, Feoktistova M, Kellert B, et al. Cellular IAPs inhibit a cryptic CD95-induced cell death by limiting RIP1 kinase recruitment. J Cell Biol. 2009;187:1037-54 pubmed publisher
	Vince J, Pantaki D, Feltham R, Mace P, Cordier S, Schmukle A, et al. TRAF2 must bind to cellular inhibitors of apoptosis for tumor necrosis factor (tnf) to efficiently activate nf-{kappa}b and to prevent tnf-induced apoptosis. J Biol Chem. 2009;284:35906-15 pubmed publisher
	Vince J, Chau D, Callus B, Wong W, Hawkins C, Schneider P, et al. TWEAK-FN14 signaling induces lysosomal degradation of a cIAP1-TRAF2 complex to sensitize tumor cells to TNFalpha. J Cell Biol. 2008;182:171-84 pubmed publisher
	Gaither A, Porter D, Yao Y, Borawski J, Yang G, Donovan J, et al. A Smac mimetic rescue screen reveals roles for inhibitor of apoptosis proteins in tumor necrosis factor-alpha signaling. Cancer Res. 2007;67:11493-8 pubmed

product information

Product ID :

ALX-803-335

Catalog Number :

ALX-803-335-C100

Product Name :

c-IAP1 monoclonal antibody (1E1-1-10)

Host species :

Rat

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

26111062, 25659587, 22332634, 21331077, 20356846, 20062539, 20038679, 19815541, 18606850, 18089776

Clone :

1E1-1-10

Isotype :

IgG2a

SPECIES REACTIVITY :

Human, Mouse

Background :

All members of the inhibitor of apoptosis proteins (IAP) family contain at least one, but usually three copies of baculovirus IAP repeat (BIR), a ~70-aa zinc binding domain, and upon overexpression suppress apoptosis. The BIR motif is capable of interacting with caspases and occluding their catalytic pockets. Certain IAPs also possess C-terminal RING domains. RING-containing proteins often act as E3 ubiquitin ligases and can catalyse the degradation of both, themselves and selected target proteins through ubiquitylation. So far, eight human IAPs have been identified: Apollon (Bruce), c-IAP1 (HIAP2; MIHB), c-IAP2 (HIAP1; MIHC), ILP-2 (Ts-IAP), Livin (ML-IAP; KIAP), NAIP, Survivin (TIAP) and XIAP (ILP-1; MIHA). c-IAP1 has been shown to inhibit specific caspases.

ALTnames :

HIAP2, BIRC-2, Baculoviral IAP repeat-containing protein-2, Cellular inhibitor of apoptosis-1, Human inhibitor of apoptosis protein-2, Inhibitor of apoptosis protein-2

Immunogen :

Synthetic peptide corresponding to aa 221-232 of mouse c-IAP1.

Buffer :

Liquid. 0.2μm-filtered solution in PBS containing 0.05% sodium azide.

Uses :

Detects a band of ~62kDa by Western blot. Not recommended for Immunohistochemistry or Immunoprecipitation.

Application Summary :

Storage :

-80°C

more info or order :

Enzo Life Sciences product webpage