LC3 monoclonal antibody (5H3) | Enzo Life Sciences ALX-803-082-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

LC3 monoclonal antibody (5H3)

catalog :

ALX-803-082-C100

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5H3

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Enzo Life Sciences product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 2	Li C, Jung S, Yang Y, Kim K, Lim J, Cheon C, et al. Inhibitory role of TRIP-Br1 oncoprotein in hypoxia-induced apoptosis in breast cancer cell lines. Int J Oncol. 2016;48:2639-46 pubmed publisher
western blot; human; fig 3	Jung S, Li C, Duan J, Lee S, Kim K, Park Y, et al. TRIP-Br1 oncoprotein inhibits autophagy, apoptosis, and necroptosis under nutrient/serum-deprived condition. Oncotarget. 2015;6:29060-75 pubmed publisher
western blot; human; fig 3	Li C, Jung S, Lee S, Jeong D, Yang Y, Kim K, et al. Nutrient/serum starvation derived TRIP-Br3 down-regulation accelerates apoptosis by destabilizing XIAP. Oncotarget. 2015;6:7522-35 pubmed
immunocytochemistry; rat western blot; rat	Sirohi K, Chalasani M, Sudhakar C, Kumari A, Radha V, Swarup G. M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells. Autophagy. 2013;9:510-27 pubmed publisher

product information

Product ID :

ALX-803-082

Catalog Number :

ALX-803-082-C100

Product Name :

LC3 monoclonal antibody (5H3)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

Clone :

5H3

Isotype :

IgG1

SPECIES REACTIVITY :

Human

Background :

Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.

ALTnames :

Microtubule-associated protein 1 light chain 3, MAP1LC3

Immunogen :

Synthetic peptide corresponding to an internal sequence identical in LC3A, LC3B and LC3C conjugated to hemocyanin.

Specificity :

Recognizes both forms of endogenous LC3.

Buffer :

Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose.

Uses :

Detects a band of ~18kDa (LC3-I; cytoplasmic form) and a band of ~16kDa (LC3-II; lipidated form) by Western blot.

Application Summary :

ICC, IHC, WB

Storage :

-80°C

more info or order :

Enzo Life Sciences product webpage