LC3B monoclonal antibody (2G6) | Enzo Life Sciences ALX-803-081-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

LC3B monoclonal antibody (2G6)

catalog :

ALX-803-081-C100

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2G6

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Enzo Life Sciences product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 6a immunocytochemistry; human; 1:1000; loading ...; fig 1c western blot; human; 1:1000; loading ...; fig 2	Cholanians A, Phan A, Ditzel E, Camenisch T, Lau S, Monks T. From the Cover: Arsenic Induces Accumulation of Î±-Synuclein: Implications for Synucleinopathies and Neurodegeneration. Toxicol Sci. 2016;153:271-81 pubmed publisher
immunocytochemistry; human; 1:250; fig 1c	Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
immunocytochemistry; rat western blot; rat	Sirohi K, Chalasani M, Sudhakar C, Kumari A, Radha V, Swarup G. M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells. Autophagy. 2013;9:510-27 pubmed publisher
	Nakajima H, Itakura M, Kubo T, Kaneshige A, Harada N, Izawa T, et al. Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Aggregation Causes Mitochondrial Dysfunction during Oxidative Stress-induced Cell Death. J Biol Chem. 2017;292:4727-4742 pubmed publisher

product information

Product ID :

ALX-803-081

Catalog Number :

ALX-803-081-C100

Product Name :

LC3B monoclonal antibody (2G6)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

Clone :

2G6

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Monkey, Hamster

Background :

Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.

ALTnames :

Microtubule-associated protein 1 light chain 3B, MAP1LC3B

Immunogen :

Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin.

Specificity :

Recognizes both forms of endogenous LC3B.

Buffer :

Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose.

Uses :

Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot.

Application Summary :

ICC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage