LC3B monoclonal antibody (5F10) | Enzo Life Sciences ALX-803-080-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

LC3B monoclonal antibody (5F10)

catalog :

ALX-803-080-C100

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5F10

reactivity :

human, mouse, rat, dogs

application :

western blot, immunocytochemistry

more info or order :

Enzo Life Sciences product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:750; loading ...; fig 4f	Bansal M, Moharir S, Sailasree S, Sirohi K, Sudhakar C, Sarathi D, et al. Optineurin promotes autophagosome formation by recruiting the autophagy-related Atg12-5-16L1 complex to phagophores containing the Wipi2 protein. J Biol Chem. 2018;293:132-147 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Parekh V, Wu L, Boyd K, Williams J, Gaddy J, Olivares Villagómez D, et al. Impaired autophagy, defective T cell homeostasis, and a wasting syndrome in mice with a T cell-specific deletion of Vps34. J Immunol. 2013;190:5086-101 pubmed publisher
immunocytochemistry; rat western blot; rat	Sirohi K, Chalasani M, Sudhakar C, Kumari A, Radha V, Swarup G. M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells. Autophagy. 2013;9:510-27 pubmed publisher
	Lim S, Jin L, Jin J, Yang C. Effect of Exendin-4 on Autophagy Clearance in Beta Cell of Rats with Tacrolimus-induced Diabetes Mellitus. Sci Rep. 2016;6:29921 pubmed publisher
	Mauvezin C, Orpinell M, Francis V, Mansilla F, Duran J, Ribas V, et al. The nuclear cofactor DOR regulates autophagy in mammalian and Drosophila cells. EMBO Rep. 2010;11:37-44 pubmed publisher
	Nishida Y, Arakawa S, Fujitani K, Yamaguchi H, Mizuta T, Kanaseki T, et al. Discovery of Atg5/Atg7-independent alternative macroautophagy. Nature. 2009;461:654-8 pubmed publisher
	Razi M, Chan E, Tooze S. Early endosomes and endosomal coatomer are required for autophagy. J Cell Biol. 2009;185:305-21 pubmed publisher

product information

Product ID :

ALX-803-080

Catalog Number :

ALX-803-080-C100

Product Name :

LC3B monoclonal antibody (5F10)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

20010805, 19794493, 19364919

Clone :

5F10

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Hamster, Dog

Background :

Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.

ALTnames :

Microtubule-associated protein 1 light chain 3B, MAP1LC3B

Immunogen :

Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin.

Specificity :

Recognizes both forms of endogenous LC3B.

Buffer :

Lyophilized from 1ml PBS containing PEG, sucrose, and 0.09% sodium azide.

Uses :

Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot.

Application Summary :

ICC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage