This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Estrogen receptor α monoclonal antibody (33)
catalog :
ALX-803-004-C050
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
33

The same clone is also sold as:
reactivity :
human, mouse, rat, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 4
Reference
Cao S, Hudnall S, Kohen F, Lu L. Measurement of estrogen receptors in intact cells by flow cytometry. Cytometry. 2000;41:109-14 pubmed
Hosli E, Rühl W, Hösli L. Histochemical and electrophysiological evidence for estrogen receptors on cultured astrocytes: colocalization with cholinergic receptors. Int J Dev Neurosci. 2000;18:101-11 pubmed
Hosli E, Hösli L. Cellular localization of estrogen receptors on neurones in various regions of cultured rat CNS: coexistence with cholinergic and galanin receptors. Int J Dev Neurosci. 1999;17:317-30 pubmed
Traish A, Ettinger R, Kim N, Marshak Rothstein A, Wotiz H. Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor. Steroids. 1990;55:196-208 pubmed
product information
Product ID :
ALX-803-004
Catalog Number :
ALX-803-004-C050
Product Name :
Estrogen receptor α monoclonal antibody (33)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
50 µg
list of Pubmed id :
11002266, 10708911, 10479067, 2360217
Clone :
33
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat, Monkey, Bovine
Background :
Steroid receptors are a large superfamily of ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Estrogen receptor (ER) has been classified into two distinct isoforms, α and β. ERα is known to interact in a ligand dependent and independent manner with several known coactivators including Receptor-Interacting Protein 140 (RIP140/ERAP140), ERAP160, and Steroid Receptor Coactivator-1 (SRC-1) to enhance transcriptional activity of target genes.
ALTnames :
ERα
Immunogen :
Synthetic peptide corresponding to aa 247-261 (E247VGMMKGGIRKDRRG261) of the DNA binding domain of ERα (estrogen receptor α). This sequence is completely conserved in many species including mouse, rat, pig, bovine, sheep, chicken and frog.
Specificity :
Recognizes both the steroid occupied and unoccupied forms of ERα.
Buffer :
Liquid. In PBS containing 0.1% sodium azide.
Uses :
Immunohistochemistry: Staining of ERα in mouse Leydig cells results in both nuclear and cytoplasmic staining. Detects a band of ~67kDa by Western blot. Does not inhibit binding of ER to nuclei and DNA.
Application Summary :
FC, ICC, IHC (FS), IHC (PS), IP, WB
Storage :
-20°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.