product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R) monoclonal antibody (M86)
catalog :
ALX-801-090-1
quantity :
1 Vial
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M86
reactivity :
human, mouse, rat, pigs
application :
western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry knockout validation
more info or order :
Enzo Life Sciences product webpage
citations: 15
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; pigs ; 1:5; fig 2
Gao H, Li S, Sun W, Yun Z, Zhang X, Song J, et al. Quantification of α-Gal Antigen Removal in the Porcine Dermal Tissue by α-Galactosidase. Tissue Eng Part C Methods. 2015;21:1197-204 pubmed publisher
  • ELISA; pigs ; loading ...; tbl 6
Lu Y, Shao A, Shan Y, Zhao H, Leiguo M, Zhang Y, et al. A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application. Sci Rep. 2018;8:15424 pubmed publisher
  • immunohistochemistry - paraffin section; pigs ; fig 7
O Connor Mooney R, Davis N, Hoey D, Hogan L, McGloughlin T, Walsh M. On the Automatic Decellularisation of Porcine Aortae: A Repeatability Study Using a Non-Enzymatic Approach. Cells Tissues Organs. 2016;201:299-318 pubmed publisher
  • ELISA; human; loading ...; fig 3a
Ramm R, Hartmann T, Tudorache I, Haverich A, Hilfiker A. No evidence for αGal epitope transfer from media containing FCS onto human endothelial cells in culture. Xenotransplantation. 2015;22:345-55 pubmed publisher
  • immunohistochemistry; pigs ; loading ...; fig 4
Diao Y, Hong J. Feasibility and safety of porcine Descemet's membrane as a carrier for generating tissue-engineered corneal endothelium. Mol Med Rep. 2015;12:1929-34 pubmed publisher
  • immunohistochemistry - paraffin section; pigs ; 1:50; loading ...; fig 2b
Santoro R, Consolo F, Spiccia M, Piola M, Kassem S, Prandi F, et al. Feasibility of pig and human-derived aortic valve interstitial cells seeding on fixative-free decellularized animal pericardium. J Biomed Mater Res B Appl Biomater. 2016;104:345-56 pubmed publisher
Apostolovic D, Tran T, Hamsten C, Starkhammar M, Cirkovic Velickovic T, van Hage M. Immunoproteomics of processed beef proteins reveal novel galactose-?-1,3-galactose-containing allergens. Allergy. 2014;69:1308-15 pubmed publisher
Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, et al. Novel human renal proximal tubular cell line for the production of complex proteins. J Biotechnol. 2014;176:29-39 pubmed publisher
Takahashi H, Chinuki Y, Tanaka A, Morita E. Laminin ?-1 and collagen ?-1 (VI) chain are galactose-?-1,3-galactose-bound allergens in beef. Allergy. 2014;69:199-207 pubmed publisher
Rodriguez I, Welsh R. Possible role of a cell surface carbohydrate in evolution of resistance to viral infections in old world primates. J Virol. 2013;87:8317-26 pubmed publisher
Wong M, Wong J, Athanasiou K, Griffiths L. Stepwise solubilization-based antigen removal for xenogeneic scaffold generation in tissue engineering. Acta Biomater. 2013;9:6492-501 pubmed publisher
Puga Yung G, Li Y, Borsig L, Millard A, Karpova M, Zhou D, et al. Complete absence of the ?Gal xenoantigen and isoglobotrihexosylceramide in ?1,3galactosyltransferase knock-out pigs. Xenotransplantation. 2012;19:196-206 pubmed publisher
Morisset M, Richard C, Astier C, Jacquenet S, Croizier A, Beaudouin E, et al. Anaphylaxis to pork kidney is related to IgE antibodies specific for galactose-alpha-1,3-galactose. Allergy. 2012;67:699-704 pubmed publisher
Naso F, Gandaglia A, Iop L, Spina M, Gerosa G. First quantitative assay of alpha-Gal in soft tissues: presence and distribution of the epitope before and after cell removal from xenogeneic heart valves. Acta Biomater. 2011;7:1728-34 pubmed publisher
Gonzalez Andrades M, de la Cruz Cardona J, Ionescu A, Campos A, Del Mar Perez M, Alaminos M. Generation of bioengineered corneas with decellularized xenografts and human keratocytes. Invest Ophthalmol Vis Sci. 2011;52:215-22 pubmed publisher
product information
Product ID :
ALX-801-090
Catalog Number :
ALX-801-090-1
Product Name :
α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R) monoclonal antibody (M86)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
1 Vial
list of Pubmed id :
25809726, 24942937, 24553072, 24180678, 23740988, 23321301, 22702471, 22494361, 21118731, 20739475
Clone :
M86
Isotype :
IgM
Product Description :
Widely described antibody against α-galactose residues with broad cross-reactivity among different species. Useful for measuring the α-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimicks in vivo reactions and can be used for cytotoxicity assays for α-Gal specific pathways (addition or presence of complement necessary). Ideal as a high-throughput screening tool for inhibitors of antibody induced cytotoxicity. Organ transplantation from pig to human result in HAR. Humans naturally produce large quantities of anti-α-Gal antibodies, which represent 1-3% of all circulating immunoglobulins and are produced by about 1% of all B cells. When pig organs or tissues are transplanted into the human body, the IgM isotype of anti-Gal binds to α-Gal epitopes, which causes activation of the complement cascade, resulting in cell lysis. This rapid activation of complement by anti-Gal IgM is an immunological barrier that poses the greatest risk of initiating HAR. The monoclonal antibody M86 to Galα1-3Gal epitopes developed by U. Galili, et al. is an essential tool in the study of human xenotransplantation and related research.
SPECIES REACTIVITY :
Mouse, Rat, Porcine
ALTnames :
α-Galactose 1,3
Immunogen :
Rabbit red blood cell membrane.
Specificity :
Recognizes synthetic and naturally produced mouse, rat and pig Galα1-3Gal epitopes on glycoproteins and glycolipids.
Buffer :
Liquid. Tissue culture supernatant.
Uses :
Functional Application: can be used for cytotoxicity assays for α-Gal specific pathways
Application Summary :
ELISA, FC, IHC (PS), WB, FUNC
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.