P-glycoprotein (human) monoclonal antibody (C494) | Enzo Life Sciences ALX-801-003-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

P-glycoprotein (human) monoclonal antibody (C494)

catalog :

ALX-801-003-C100

quantity :

1 ml

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C494

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Enzo Life Sciences product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:100	van der Heyden S, Chiers K, Vercauteren G, Daminet S, Wegge B, Paepe D, et al. Expression of multidrug resistance-associated P-glycoprotein in feline tumours. J Comp Pathol. 2011;144:164-9 pubmed publisher
immunohistochemistry - paraffin section; human	Kwan P, Li H, Al Jufairi E, Abdulla R, Gonzales M, Kaye A, et al. Association between temporal lobe P-glycoprotein expression and seizure recurrence after surgery for pharmacoresistant temporal lobe epilepsy. Neurobiol Dis. 2010;39:192-7 pubmed publisher
	Mahdi Z, Synal Hermanns U, Yoker A, Locher K, Stieger B. Role of Multidrug Resistance Protein 3 in Antifungal-Induced Cholestasis. Mol Pharmacol. 2016;90:23-34 pubmed publisher
	Le Vee M, Jouan E, Noël G, Stieger B, Fardel O. Polarized location of SLC and ABC drug transporters in monolayer-cultured human hepatocytes. Toxicol In Vitro. 2015;29:938-46 pubmed publisher
	PoÅºniak B, Pawlak A, ObmiÅ„ska Mrukowicz B. Flow cytometric assessment of P-glycoprotein and multidrug resistance-associated protein activity and expression in canine lymphoma. In Vivo. 2015;29:149-53 pubmed
	Feldmann M, Asselin M, Liu J, Wang S, McMahon A, Anton Rodriguez J, et al. P-glycoprotein expression and function in patients with temporal lobe epilepsy: a case-control study. Lancet Neurol. 2013;12:777-85 pubmed publisher
	Honscha K, Schirmer A, Reischauer A, Schoon H, Einspanier A, Gabel G. Expression of ABC-transport proteins in canine mammary cancer: consequences for chemotherapy. Reprod Domest Anim. 2009;44 Suppl 2:218-23 pubmed publisher
	Schroeijers A, Reurs A, Scheffer G, Stam A, de Jong M, Rustemeyer T, et al. Up-regulation of drug resistance-related vaults during dendritic cell development. J Immunol. 2002;168:1572-8 pubmed
	Nuti S, Mehdi A, Rao U. Activation of the human P-glycoprotein ATPase by trypsin. Biochemistry. 2000;39:3424-32 pubmed
	Ng I, Liu C, Fan S, Ng M. Expression of P-glycoprotein in hepatocellular carcinoma. A determinant of chemotherapy response. Am J Clin Pathol. 2000;113:355-63 pubmed
	Wyman J, Hornstein A, Meitner P, Mak S, Verdier P, Block J, et al. Multidrug resistance-1 and p-glycoprotein in human chondrosarcoma cell lines: expression correlates with decreased intracellular doxorubicin and in vitro chemoresistance. J Orthop Res. 1999;17:935-40 pubmed
	Jimenez R, Zalupski M, Frank J, Du W, Ryan J, Lucas D. Multidrug resistance phenotype in high grade soft tissue sarcoma: correlation of P-glycoprotein immunohistochemistry with pathologic response to chemotherapy. Cancer. 1999;86:976-81 pubmed
	Rao V, Dahlheimer J, Bardgett M, Snyder A, Finch R, Sartorelli A, et al. Choroid plexus epithelial expression of MDR1 P glycoprotein and multidrug resistance-associated protein contribute to the blood-cerebrospinal-fluid drug-permeability barrier. Proc Natl Acad Sci U S A. 1999;96:3900-5 pubmed
	Lacueva F, Teruel A, Calpena R, Medrano J, Mayol M, Perez Vazquez M, et al. Detection of P-glycoprotein in frozen and paraffin-embedded gastric adenocarcinoma tissues using a panel of monoclonal antibodies. Histopathology. 1998;32:328-34 pubmed
	Rao V, Anthony D, Piwnica Worms D. MDR1 gene-specific monoclonal antibody C494 cross-reacts with pyruvate carboxylase. Cancer Res. 1994;54:1536-41 pubmed

product information

Product ID :

ALX-801-003

Catalog Number :

ALX-801-003-C100

Product Name :

P-glycoprotein (human) monoclonal antibody (C494)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

1 ml

list of Pubmed id :

25862123, 23786896, 11823484, 10727237, 10705815, 10632461, 10491523, 10097135, 9602329, 7511043

Clone :

C494

Isotype :

IgG2a

SPECIES REACTIVITY :

Human

ALTnames :

ABCB1, CD243, P-glycoprotein 1

Immunogen :

SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary cell line and a human leukemic cell line.

Specificity :

Recognizes a gene-specific, internal cellular epitope present only on the human MDR1 isoform of P-glycoprotein.

Buffer :

Liquid. In PBS containing BSA and 0.1% sodium azide.

Uses :

Flow Cytometry: cell permeabilization required. Immunocytochemistry: Cytospin preparations. Immunoprecipitation: The C494 MAb can be used to immunoprecipitate P-glycoprotein from lysates of metabolically or surface radiolabelled cells.

Application Summary :

FC, ICC, IHC (FS), IHC (PS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage