P-glycoprotein monoclonal antibody (C219) | Enzo Life Sciences ALX-801-002-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

P-glycoprotein monoclonal antibody (C219)

catalog :

ALX-801-002-C100

quantity :

1 ml

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C219

reactivity :

human, mouse, rat, dogs, domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Enzo Life Sciences product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; loading ...; fig 3a western blot; human; 1:300; loading ...; fig 2b	Mieszala K, Rudewicz M, Gomulkiewicz A, Ratajczak Wielgomas K, Grzegrzolka J, Dziegiel P, et al. Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol. Oncol Lett. 2018;15:5825-5832 pubmed publisher
western blot; human; fig 7a	Sayyed K, Vee M, Abdel Razzak Z, Jouan E, Stieger B, Denizot C, et al. Alteration of human hepatic drug transporter activity and expression by cigarette smoke condensate. Toxicology. 2016;363-364:58-71 pubmed publisher
immunocytochemistry; mouse; 1:50; fig 3c	Kanda M, Nagai T, Takahashi T, Liu M, Kondou N, Naito A, et al. Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction. PLoS ONE. 2016;11:e0156562 pubmed publisher
western blot; human; loading ...; fig 5b	Mahdi Z, Synal Hermanns U, Yoker A, Locher K, Stieger B. Role of Multidrug Resistance Protein 3 in Antifungal-Induced Cholestasis. Mol Pharmacol. 2016;90:23-34 pubmed publisher
western blot; domestic sheep; 1:100; loading ...; fig 1	Wilkens M, MatÃ© L, Schnepel N, Klinger S, Muscher Banse A, Ballent M, et al. Influence of 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 on expression of P-glycoprotein and cytochrome P450 3A in sheep. J Steroid Biochem Mol Biol. 2016;164:271-276 pubmed publisher
western blot; human; loading ...; fig 3A	Nourbakhsh M, Jaafari M, Lage H, Abnous K, Mosaffa F, Badiee A, et al. Nanolipoparticles-mediated MDR1 siRNA delivery reduces doxorubicin resistance in breast cancer cells and silences MDR1 expression in xenograft model of human breast cancer. Iran J Basic Med Sci. 2015;18:385-92 pubmed
immunocytochemistry; human; 1:100 western blot; human	Bernd A, Ott M, Ishikawa H, Schroten H, Schwerk C, Fricker G. Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier. Pharm Res. 2015;32:2973-82 pubmed publisher
western blot; human; 1:250	Doherty B, Lawlor D, Gillet J, Gottesman M, O Leary J, Stordal B. Collateral sensitivity to cisplatin in KB-8-5-11 drug-resistant cancer cells. Anticancer Res. 2014;34:503-7 pubmed
western blot; mouse	Aleksunes L, Xu J, Lin E, Wen X, Goedken M, Slitt A. Pregnancy represses induction of efflux transporters in livers of type I diabetic mice. Pharm Res. 2013;30:2209-20 pubmed publisher
western blot; human; 1:200	Vinot C, Gavard L, Treluyer J, Manceau S, Courbon E, Scherrmann J, et al. Placental transfer of maraviroc in an ex vivo human cotyledon perfusion model and influence of ABC transporter expression. Antimicrob Agents Chemother. 2013;57:1415-20 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:25	Brenn A, Grube M, Peters M, Fischer A, Jedlitschky G, Kroemer H, et al. Beta-Amyloid Downregulates MDR1-P-Glycoprotein (Abcb1) Expression at the Blood-Brain Barrier in Mice. Int J Alzheimers Dis. 2011;2011:690121 pubmed publisher
	Coculova M, Imrichova D, Seres M, Messingerová L, Boháčová V, Sulova Z, et al. The expression of P-glycoprotein in leukemia cells is associated with the upregulated expression of nestin, a class 6 filament protein. Leuk Res. 2016;48:32-9 pubmed publisher
	Ghoneim R, Piquette Miller M. Endotoxin-Mediated Downregulation of Hepatic Drug Transporters in HIV-1 Transgenic Rats. Drug Metab Dispos. 2016;44:709-19 pubmed publisher
	Kumar J, Wei B, Madigan J, Simpson R, Hall M, Gottesman M. Bioluminescent imaging of ABCG2 efflux activity at the blood-placenta barrier. Sci Rep. 2016;6:20418 pubmed publisher
	Chapy H, SaubamÃ©a B, Tournier N, Bourasset F, Behar Cohen F, DeclÃ¨ves X, et al. Blood-brain and retinal barriers show dissimilar ABC transporter impacts and concealed effect of P-glycoprotein on a novel verapamil influx carrier. Br J Pharmacol. 2016;173:497-510 pubmed publisher
	Wojtowicz K, Januchowski R, Nowicki M, Zabel M. Inhibition of protein glycosylation reverses the MDR phenotype of cancer cell lines. Biomed Pharmacother. 2015;74:49-56 pubmed publisher
	Imrichova D, Messingerová L, Seres M, Kavcová H, Pavlikova L, Coculova M, et al. Selection of resistant acute myeloid leukemia SKM-1 and MOLM-13 cells by vincristine-, mitoxantrone- and lenalidomide-induced upregulation of P-glycoprotein activity and downregulation of CD33 cell surface exposure. Eur J Pharm Sci. 2015;77:29-39 pubmed publisher
	Willyerd F, Empey P, Philbrick A, Ikonomovic M, Puccio A, Kochanek P, et al. Expression of ATP-Binding Cassette Transporters B1 and C1 after Severe Traumatic Brain Injury in Humans. J Neurotrauma. 2016;33:226-31 pubmed publisher
	Jacob A, Potin S, Chapy H, Crete D, Glacial F, Ganeshamoorthy K, et al. Aryl hydrocarbon receptor regulates CYP1B1 but not ABCB1 and ABCG2 in hCMEC/D3 human cerebral microvascular endothelial cells after TCDD exposure. Brain Res. 2015;1613:27-36 pubmed publisher
	Ellis K, Marlin J, Taylor T, Fitting S, Hauser K, Rice G, et al. The effects of human immunodeficiency virus infection on the expression of the drug efflux proteins P-glycoprotein and breast cancer resistance protein in a human intestine model. J Pharm Pharmacol. 2015;67:178-88 pubmed publisher
	Bexten M, Oswald S, Grube M, Jia J, Graf T, Zimmermann U, et al. Expression of drug transporters and drug metabolizing enzymes in the bladder urothelium in man and affinity of the bladder spasmolytic trospium chloride to transporters likely involved in its pharmacokinetics. Mol Pharm. 2015;12:171-8 pubmed publisher
	Stremmel W, Staffer S, Wannhoff A, Pathil A, Chamulitrat W. Plasma membrane phospholipase A2 controls hepatocellular fatty acid uptake and is responsive to pharmacological modulation: implications for nonalcoholic steatohepatitis. FASEB J. 2014;28:3159-70 pubmed publisher
	O Brien F, Clarke G, Dinan T, Cryan J, Griffin B. Human P-glycoprotein differentially affects antidepressant drug transport: relevance to blood-brain barrier permeability. Int J Neuropsychopharmacol. 2013;16:2259-72 pubmed publisher
	Feldmann M, Asselin M, Liu J, Wang S, McMahon A, Anton Rodriguez J, et al. P-glycoprotein expression and function in patients with temporal lobe epilepsy: a case-control study. Lancet Neurol. 2013;12:777-85 pubmed publisher
	Brenn A, Grube M, Jedlitschky G, Fischer A, Strohmeier B, Eiden M, et al. St. John's Wort reduces beta-amyloid accumulation in a double transgenic Alzheimer's disease mouse model-role of P-glycoprotein. Brain Pathol. 2014;24:18-24 pubmed publisher
	Lee W, Chakraborty P, Thevenod F. Pituitary homeobox 2 (PITX2) protects renal cancer cell lines against doxorubicin toxicity by transcriptional activation of the multidrug transporter ABCB1. Int J Cancer. 2013;133:556-67 pubmed publisher
	Gibson C, Hossain M, Richardson J, Aleksunes L. Inflammatory regulation of ATP binding cassette efflux transporter expression and function in microglia. J Pharmacol Exp Ther. 2012;343:650-60 pubmed publisher
	Bruyère A, Declèves X, Bouzom F, Ball K, Marques C, Treton X, et al. Effect of variations in the amounts of P-glycoprotein (ABCB1), BCRP (ABCG2) and CYP3A4 along the human small intestine on PBPK models for predicting intestinal first pass. Mol Pharm. 2010;7:1596-607 pubmed publisher
	Bottova I, Sauder U, Olivieri V, Hehl A, Sonda S. The P-glycoprotein inhibitor GF120918 modulates Ca2+-dependent processes and lipid metabolism in Toxoplasma gondii. PLoS ONE. 2010;5:e10062 pubmed publisher
	Borska S, Sopel M, Chmielewska M, Zabel M, Dziegiel P. Quercetin as a potential modulator of P-glycoprotein expression and function in cells of human pancreatic carcinoma line resistant to daunorubicin. Molecules. 2010;15:857-70 pubmed publisher

product information

Product ID :

ALX-801-002

Catalog Number :

ALX-801-002-C100

Product Name :

P-glycoprotein monoclonal antibody (C219)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

1 ml

list of Pubmed id :

26349962, 26002042, 24719358, 23931269, 23786896, 23701205, 21660212, 20604570, 20386707, 20335952

Clone :

C219

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Primate, Dog

ALTnames :

ABCB1, CD243, P-glycoprotein 1

Immunogen :

SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line.

Specificity :

Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein.

Buffer :

Liquid. In 0.01M PBS containing 1% BSA and 0.1% sodium azide.

Uses :

Flow Cytometry: cell permeabilization required. Detects bands of ~170kDa (MDR1 P-glycoprotein) and of ~140kDa (MDR3 P-glycoprotein) by Western blot. Cited applications include ELISA, IF, FC, and WB

Application Summary :

ICC, IHC (FS), IHC (PS), IP

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage