Cardif (human) polyclonal antibody (AT107) | Enzo Life Sciences ALX-210-929-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Cardif (human) polyclonal antibody (AT107)

catalog :

ALX-210-929-C100

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; fig 3	Chan Y, Gack M. A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity. Nat Immunol. 2016;17:523-30 pubmed publisher
	Hertzog J, Dias Júnior A, Rigby R, Donald C, Mayer A, Sezgin E, et al. Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling. Eur J Immunol. 2018;48:1120-1136 pubmed publisher
	DecorsiÃ¨re A, Mueller H, van Breugel P, Abdul F, Gerossier L, Beran R, et al. Hepatitis B virus X protein identifies the Smc5/6 complex as a host restriction factor. Nature. 2016;531:386-9 pubmed publisher

product information

Product ID :

ALX-210-929

Catalog Number :

ALX-210-929-C100

Product Name :

Cardif (human) polyclonal antibody (AT107)

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

20044805, 18756281, 18725224, 18689426, 17942531, 16858409

SPECIES REACTIVITY :

Human

Background :

RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.

ALTnames :

CARD adapter inducing interferon-β, IPS-1, Interferon-β promoter stimulator protein 1, MAVS, Mitochondrial antiviral signalling protein, VISA, Virus-induced signalling adapter

Immunogen :

Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).

Buffer :

Liquid. In PBS containing 0.02% sodium azide.

Uses :

Detects a band of ~65kDa by Western blot.

Application Summary :

ICC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage