α-Actinin-4 polyclonal antibody | Enzo Life Sciences ALX-210-356-C050 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

α-Actinin-4 polyclonal antibody

catalog :

ALX-210-356-C050

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1d	Miikkulainen P, Högel H, Rantanen K, Suomi T, Kouvonen P, Elo L, et al. HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma. Cancer Metab. 2017;5:5 pubmed publisher
	Djuric I, Siebrasse J, Schulze U, Granado D, SchlÃ¼ter M, Kubitscheck U, et al. The C-terminal domain controls the mobility of Crumbs 3 isoforms. Biochim Biophys Acta. 2016;1863:1208-17 pubmed publisher
	Sun Y, Jaldin Fincati J, Liu Z, Bilan P, Klip A. A complex of Rab13 with MICAL-L2 and Î±-actinin-4 is essential for insulin-dependent GLUT4 exocytosis. Mol Biol Cell. 2016;27:75-89 pubmed publisher

product information

Product ID :

ALX-210-356

Catalog Number :

ALX-210-356-C050

Product Name :

α-Actinin-4 polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

26538022, 25944910, 25860875, 16105041, 15772161, 15031339

SPECIES REACTIVITY :

Human, Mouse, Rat, Porcine, Chinese hamster

Background :

α-Actinin-4 is an actin-bundling protein of ~100kDa that is associated with cell motility, endocytosis, and cancer invasion. The α-actinin family comprises two non-muscle isoforms (α-actinin-1 and -4) and two skeletal muscle isoforms (α-actinin-2 and -3), with α-actinin-2 being also expressed in cardiac muscle. While α-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the α-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the α-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. α-actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, α-actinin-4 forms a ternary complex with Ca2 / Calmodulin-dependent protein kinase II and Densin-180, a protein of postsynaptic densities in CNS neurons. Ca2 -dependent association of α-actinin-4 with E3KARP is required for Ca2 -dependent inhibition of the Na / H exchanger 3 (NHE3).

Immunogen :

Synthetic peptide corresponding to aa 28-38 (M28GDYMAQEDDW38) of human and rat actinin-4 and to aa 29-39 of mouse actinin-4.

Buffer :

Liquid. In PBS containing 1mg/ml BSA and 0.01% sodium azide.

Application Summary :

IHC (FS), IHC (PS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage