Glucocorticoid receptor β (human) polyclonal antibody | Enzo Life Sciences ALX-210-154-R050 product information

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product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Glucocorticoid receptor β (human) polyclonal antibody

catalog :

ALX-210-154-R050

quantity :

50 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

product information

Product ID :

ALX-210-154

Catalog Number :

ALX-210-154-R050

Product Name :

Glucocorticoid receptor β (human) polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

50 µl

list of Pubmed id :

9348235

SPECIES REACTIVITY :

Human

Background :

Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR.The human GR exists in two forms, α and β, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that the α and β forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94kDa GRα isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90kDa GRβ isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GRβ might function as a dominant negative inhibitor of GRα activity.

ALTnames :

GRβ

Immunogen :

Synthetic peptide corresponding to aa 728-742 (N728VMWLKPESTSHTLI742) of human GRβ (glucocorticoid receptor β).

Buffer :

Liquid. In PBS containing 0.05% sodium azide.

Uses :

Immunocytochemical staining of GRβ in HeLa cells with Prod. No. ALX-210-154 results in nuclear staining. Detects a band of ~90kDa by Western blot.

Application Summary :

ICC, IP, WB

Storage :

-20°C