This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
protein
product name :
Sp1 protein (human), (recombinant) (HA-tag)
catalog :
ALX-201-106-R050
quantity :
50 µl
citations: 5
Reference
Sakaguchi M, Sonegawa H, Nukui T, Sakaguchi Y, Miyazaki M, Namba M, et al. Bifurcated converging pathways for high Ca2+- and TGFbeta-induced inhibition of growth of normal human keratinocytes. Proc Natl Acad Sci U S A. 2005;102:13921-6 pubmed
Abdelrahim M, Liu S, Safe S. Induction of endoplasmic reticulum-induced stress genes in Panc-1 pancreatic cancer cells is dependent on Sp proteins. J Biol Chem. 2005;280:16508-13 pubmed
Suske G. The Sp-family of transcription factors. Gene. 1999;238:291-300 pubmed
Braun H, Suske G. Vectors for inducible expression of dual epitope-tagged proteins in insect cells. Biotechniques. 1999;26:1038-40, 1042 pubmed
Kadonaga J, Carner K, Masiarz F, Tjian R. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Cell. 1987;51:1079-90 pubmed
product information
Product ID :
ALX-201-106
Catalog Number :
ALX-201-106-R050
Product Name :
Sp1 protein (human), (recombinant) (HA-tag)
Product Type :
Protein
Size :
50 µl
list of Pubmed id :
16172401, 15760841, 10570957, 10376134, 3319186
Product Description :
The protein may be employed in the following fields of research:. i) Promoter studies: Identification of Sp1 binding promoter elements by bandshift or DNase1 footprint assays. ii) Detection of Sp1 interacting proteins.
Background :
The ubiquitously expressed Sp1 was the first identified and cloned member of the Sp-family of mammalian transcription factors. The 785 aa human protein contains an 81 aa DNA-binding domain consisting of three C2H2-type zinc fingers close to the C-terminus. Two glutamine-rich activation domains are present adjacent to serine/threonine stretches in the N-terminal part of the protein. Sp1 recognizes specifically G-rich elements such as the GC-box (GGGGCGGGG) and the GT/CACC-box (GGTGTGGGG) found in housekeeping as well as in many tissue-specific and viral genes.
Buffer :
Liquid. In 50mM TRIS-HCl, pH 7.9, containing 420mM KCl, 0.1% NP-40, 12.5mM MgCl2 and 20% glycerol.
Uses :
EMSA promoter characterization, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
Storage :
-80°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.