product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Bassoon monoclonal antibody (SAP7F407)
catalog :
ADI-VAM-PS003-F
quantity :
200 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SAP7F407
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
citations: 59
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:500; loading ...; fig s7a
Suzuki K, Elegheert J, Song I, Sasakura H, Senkov O, Matsuda K, et al. A synthetic synaptic organizer protein restores glutamatergic neuronal circuits. Science. 2020;369: pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 2a
Banerjee A, Lee J, Nemcova P, Liu C, Kaeser P. Synaptotagmin-1 is the Ca2+ sensor for fast striatal dopamine release. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 9e1
Nakamoto C, Konno K, Miyazaki T, Nakatsukasa E, Natsume R, Abe M, et al. Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain. J Comp Neurol. 2019;: pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 6e
Rubio Fernández M, Uribe M, Vicente Tejedor J, Germain F, Susín Lara C, Quereda C, et al. Impairment of photoreceptor ribbon synapses in a novel Pomt1 conditional knockout mouse model of dystroglycanopathy. Sci Rep. 2018;8:8543 pubmed publisher
  • immunohistochemistry; mouse; fig 1a
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser P. Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites. Cell. 2018;172:706-718.e15 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; tbl 1
Sai K, Wang S, Kaito A, Fujiwara T, Maruo T, Itoh Y, et al. Multiple roles of afadin in the ultrastructural morphogenesis of mouse hippocampal mossy fiber synapses. J Comp Neurol. 2017;525:2719-2734 pubmed publisher
  • immunocytochemistry; rat; 1:1000; loading ...; fig 4a
Goodman L, Baddeley D, Ambroziak W, Waites C, Garner C, Soeller C, et al. N-terminal SAP97 isoforms differentially regulate synaptic structure and postsynaptic surface pools of AMPA receptors. Hippocampus. 2017;27:668-682 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...
Luke M, LeVatte T, Rutishauser U, Tremblay F, Clarke D. Polysialylated Neural Cell Adhesion Molecule Protects Against Light-Induced Retinal Degeneration. Invest Ophthalmol Vis Sci. 2016;57:5066-5075 pubmed publisher
  • immunocytochemistry; rat; 1:1000; loading ...; fig 6a
Ampuero E, Jury N, Hartel S, Marzolo M, van Zundert B. Interfering of the Reelin/ApoER2/PSD95 Signaling Axis Reactivates Dendritogenesis of Mature Hippocampal Neurons. J Cell Physiol. 2017;232:1187-1199 pubmed publisher
  • immunocytochemistry; rat; fig 5h
Loh K, Stawski P, Draycott A, Udeshi N, Lehrman E, Wilton D, et al. Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts. Cell. 2016;166:1295-1307.e21 pubmed publisher
  • immunocytochemistry; mouse; 1:600; fig 2
Nishimune H, Badawi Y, Mori S, Shigemoto K. Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice. Sci Rep. 2016;6:27935 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig s3
Heise C, Schroeder J, Schoen M, Halbedl S, Reim D, Woelfle S, et al. Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus. Front Cell Neurosci. 2016;10:106 pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; fig 4a
Gresa Arribas N, Planaguma J, Petit Pedrol M, Kawachi I, Katada S, Glaser C, et al. Human neurexin-3α antibodies associate with encephalitis and alter synapse development. Neurology. 2016;86:2235-42 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 6
Wang X, Bey A, Katz B, Badea A, Kim N, David L, et al. Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. Nat Commun. 2016;7:11459 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:1000; loading ...
Pinilla I, Fernández Sánchez L, Segura F, Sánchez Cano A, Tamarit J, Fuentes Broto L, et al. Long time remodeling during retinal degeneration evaluated by optical coherence tomography, immunocytochemistry and fundus autofluorescence. Exp Eye Res. 2016;150:122-34 pubmed publisher
  • immunohistochemistry; mouse; fig 8
Du M, Otalora L, Martin A, Moiseyev G, Vanlandingham P, Wang Q, et al. Transgenic Mice Overexpressing Serum Retinol-Binding Protein Develop Progressive Retinal Degeneration through a Retinoid-Independent Mechanism. Mol Cell Biol. 2015;35:2771-89 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200
Jacobi A, Loy K, Schmalz A, Hellsten M, Umemori H, Kerschensteiner M, et al. FGF22 signaling regulates synapse formation during post-injury remodeling of the spinal cord. EMBO J. 2015;34:1231-43 pubmed publisher
  • western blot; human
Cuesto G, Jordán Álvarez S, Enriquez Barreto L, Ferrús A, Morales M, Acebes A. GSK3β inhibition promotes synaptogenesis in Drosophila and mammalian neurons. PLoS ONE. 2015;10:e0118475 pubmed publisher
  • immunohistochemistry; mouse; 1:500; fig 9E
Pérez de Sevilla Müller L, Sargoy A, Fernández Sánchez L, Rodriguez A, Liu J, Cuenca N, et al. Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina. J Comp Neurol. 2015;523:1443-60 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100
Chand K, Lee K, Schenning M, Lavidis N, Noakes P. Loss of β2-laminin alters calcium sensitivity and voltage-gated calcium channel maturation of neurotransmission at the neuromuscular junction. J Physiol. 2015;593:245-65 pubmed publisher
  • immunohistochemistry - free floating section; rat; 1 ug/ml; loading ...; fig 1d
Chounlamountry K, Castets F, Tell F, Kessler J. The excitatory amino acid carrier 1 (EAAC1) in the rat nucleus of the solitary tract: subcellular localization suggests no major role in glutamate clearance. Brain Struct Funct. 2016;221:1113-24 pubmed publisher
  • immunohistochemistry; mouse
Ishiyama S, Schmidt H, Cooper B, Brose N, Eilers J. Munc13-3 superprimes synaptic vesicles at granule cell-to-basket cell synapses in the mouse cerebellum. J Neurosci. 2014;34:14687-96 pubmed publisher
  • immunohistochemistry; rat; 1:250
Bourque S, Kuny S, Reyes L, Davidge S, Sauve Y. Prenatal hypoxia is associated with long-term retinal dysfunction in rats. PLoS ONE. 2013;8:e61861 pubmed publisher
  • western blot; mouse
Mandemakers W, Abuhatzira L, Xu H, Caromile L, Hébert S, Snellinx A, et al. Co-regulation of intragenic microRNA miR-153 and its host gene Ia-2 ?: identification of miR-153 target genes with functions related to IA-2? in pancreas and brain. Diabetologia. 2013;56:1547-56 pubmed publisher
  • immunocytochemistry; rat
Taylor A, Wu J, Tai H, Schuman E. Axonal translation of ?-catenin regulates synaptic vesicle dynamics. J Neurosci. 2013;33:5584-9 pubmed publisher
  • immunohistochemistry; rat; 1:100
Petralia R, Schwartz C, Wang Y, Mattson M, Yao P. Subcellular localization of Patched and Smoothened, the receptors for Sonic hedgehog signaling, in the hippocampal neuron. J Comp Neurol. 2011;519:3684-99 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000
O Brien B, Caldwell J, Ehring G, Bumsted O Brien K, Luo S, Levinson S. Tetrodotoxin-resistant voltage-gated sodium channels Na(v)1.8 and Na(v)1.9 are expressed in the retina. J Comp Neurol. 2008;508:940-51 pubmed publisher
  • immunohistochemistry; rat
Hagiwara A, Fukazawa Y, Deguchi Tawarada M, Ohtsuka T, Shigemoto R. Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labeling. J Comp Neurol. 2005;489:195-216 pubmed
Emperador Melero J, Huson V, Van Weering J, Bollmann C, Fischer von Mollard G, Toonen R, et al. Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi. Nat Commun. 2018;9:3421 pubmed publisher
Richter K, Revelo N, Seitz K, Helm M, Sarkar D, Saleeb R, et al. Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy. EMBO J. 2018;37:139-159 pubmed publisher
Goo M, Sancho L, Slepak N, Boassa D, Deerinck T, Ellisman M, et al. Activity-dependent trafficking of lysosomes in dendrites and dendritic spines. J Cell Biol. 2017;216:2499-2513 pubmed publisher
Kawabe H, Mitkovski M, Kaeser P, Hirrlinger J, Opazo F, Nestvogel D, et al. ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones. J Cell Biol. 2017;216:1143-1161 pubmed publisher
Yokoyama T, Saino T, Nakamuta N, Kusakabe T, Yamamoto Y. Three-dimensional architectures of P2X2-/P2X3-immunoreactive afferent nerve terminals in the rat carotid body as revealed by confocal laser scanning microscopy. Histochem Cell Biol. 2016;146:479-88 pubmed publisher
Vicidomini C, Ponzoni L, Lim D, Schmeisser M, Reim D, Morello N, et al. Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice. Mol Psychiatry. 2017;22:689-702 pubmed publisher
Spaulding E, Sleigh J, Morelli K, Pinter M, Burgess R, Seburn K. Synaptic Deficits at Neuromuscular Junctions in Two Mouse Models of Charcot-Marie-Tooth Type 2d. J Neurosci. 2016;36:3254-67 pubmed publisher
Jimeno D, Gómez C, Calzada N, de la Villa P, Lillo C, Santos E. RASGRF2 controls nuclear migration in postnatal retinal cone photoreceptors. J Cell Sci. 2016;129:729-42 pubmed publisher
Kawabata S, Takano M, Numasawa Kuroiwa Y, Itakura G, Kobayashi Y, Nishiyama Y, et al. Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury. Stem Cell Reports. 2016;6:1-8 pubmed publisher
Shin J, Eom Y, Yu W, Grzywacz N, CRAFT C, Lee E. TIMP-1 affects the spatial distribution of dendritic processes of second-order neurons in a rat model of Retinitis Pigmentosa. Exp Eye Res. 2015;140:41-52 pubmed publisher
Lee C, Huang C, Hsu K. The phospholipid-binding protein SESTD1 negatively regulates dendritic spine density by interfering with Rac1-Trio8 signaling pathway. Sci Rep. 2015;5:13250 pubmed publisher
Körber C, Horstmann H, Venkataramani V, Herrmannsdörfer F, Kremer T, Kaiser M, et al. Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover. Neuron. 2015;87:521-33 pubmed publisher
Laguna A, Schintu N, Nobre A, Alvarsson A, Volakakis N, Jacobsen J, et al. Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinson's disease. Nat Neurosci. 2015;18:826-35 pubmed publisher
Yamagata A, Yoshida T, Sato Y, Goto Ito S, Uemura T, Maeda A, et al. Mechanisms of splicing-dependent trans-synaptic adhesion by PTPδ-IL1RAPL1/IL-1RAcP for synaptic differentiation. Nat Commun. 2015;6:6926 pubmed publisher
Segovia Miranda F, Serrano F, Dyrda A, Ampuero E, Retamal C, Bravo Zehnder M, et al. Pathogenicity of lupus anti-ribosomal P antibodies: role of cross-reacting neuronal surface P antigen in glutamatergic transmission and plasticity in a mouse model. Arthritis Rheumatol. 2015;67:1598-610 pubmed publisher
Park D, Cerrone M, Morley G, Vasquez C, Fowler S, Liu N, et al. Genetically engineered SCN5A mutant pig hearts exhibit conduction defects and arrhythmias. J Clin Invest. 2015;125:403-12 pubmed publisher
Takaki F, Nakamuta N, Kusakabe T, Yamamoto Y. Sympathetic and sensory innervation of small intensely fluorescent (SIF) cells in rat superior cervical ganglion. Cell Tissue Res. 2015;359:441-451 pubmed publisher
Samuel M, Voinescu P, Lilley B, de Cabo R, Foretz M, Viollet B, et al. LKB1 and AMPK regulate synaptic remodeling in old age. Nat Neurosci. 2014;17:1190-7 pubmed publisher
Moscato E, Peng X, Jain A, Parsons T, Dalmau J, Balice Gordon R. Acute mechanisms underlying antibody effects in anti-N-methyl-D-aspartate receptor encephalitis. Ann Neurol. 2014;76:108-19 pubmed publisher
Dembla M, Wahl S, Katiyar R, Schmitz F. ArfGAP3 is a component of the photoreceptor synaptic ribbon complex and forms an NAD(H)-regulated, redox-sensitive complex with RIBEYE that is important for endocytosis. J Neurosci. 2014;34:5245-60 pubmed publisher
Yam P, Pincus Z, Gupta G, Bashkurov M, Charron F, Pelletier L, et al. N-cadherin relocalizes from the periphery to the center of the synapse after transient synaptic stimulation in hippocampal neurons. PLoS ONE. 2013;8:e79679 pubmed publisher
Wu F, Li R, Umino Y, Kaczynski T, Sapkota D, Li S, et al. Onecut1 is essential for horizontal cell genesis and retinal integrity. J Neurosci. 2013;33:13053-65, 13065a pubmed publisher
Nishimune H, Numata T, Chen J, Aoki Y, Wang Y, Starr M, et al. Active zone protein Bassoon co-localizes with presynaptic calcium channel, modifies channel function, and recovers from aging related loss by exercise. PLoS ONE. 2012;7:e38029 pubmed publisher
Djakovic S, Marquez Lona E, Jakawich S, Wright R, Chu C, Sutton M, et al. Phosphorylation of Rpt6 regulates synaptic strength in hippocampal neurons. J Neurosci. 2012;32:5126-31 pubmed publisher
Kantardzhieva A, Peppi M, Lane W, Sewell W. Protein composition of immunoprecipitated synaptic ribbons. J Proteome Res. 2012;11:1163-74 pubmed publisher
Chen J, Mizushige T, Nishimune H. Active zone density is conserved during synaptic growth but impaired in aged mice. J Comp Neurol. 2012;520:434-52 pubmed publisher
Schrof S, Staudt T, Rittweger E, Wittenmayer N, Dresbach T, Engelhardt J, et al. STED nanoscopy with mass-produced laser diodes. Opt Express. 2011;19:8066-72 pubmed publisher
Chen J, Billings S, Nishimune H. Calcium channels link the muscle-derived synapse organizer laminin ?2 to Bassoon and CAST/Erc2 to organize presynaptic active zones. J Neurosci. 2011;31:512-25 pubmed publisher
Fairless R, Masius H, Rohlmann A, Heupel K, Ahmad M, Reissner C, et al. Polarized targeting of neurexins to synapses is regulated by their C-terminal sequences. J Neurosci. 2008;28:12969-81 pubmed publisher
Lefebvre J, Zhang Y, Meister M, Wang X, Sanes J. gamma-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina. Development. 2008;135:4141-51 pubmed publisher
Medvedeva Y, Kim M, Usachev Y. Mechanisms of prolonged presynaptic Ca2+ signaling and glutamate release induced by TRPV1 activation in rat sensory neurons. J Neurosci. 2008;28:5295-311 pubmed publisher
product information
Product ID :
ADI-VAM-PS003
Catalog Number :
ADI-VAM-PS003-F
Product Name :
Bassoon monoclonal antibody (SAP7F407)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
200 µg
list of Pubmed id :
26724902, 26277580, 26272757, 25915474, 25908590, 25764078, 25086610, 24916964, 24719103, 24223993
Clone :
SAP7F407
Isotype :
IgG2a
SPECIES REACTIVITY :
Mouse, Rat
Background :
Bassoon is a 420 kDa protein that localizes at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.
Immunogen :
Recombinant rat Bassoon.
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~400kDa by Western blot.
Application Summary :
ICC, IF, IHC, IP, WB
Storage :
-20°C
more info or order :
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.