LAMP1 monoclonal antibody (Ly1C6) | Enzo Life Sciences ADI-VAM-EN001-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

LAMP1 monoclonal antibody (Ly1C6)

catalog :

ADI-VAM-EN001-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ly1C6

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; 1:100; loading ...; fig 8c	Wolfes A, Ahmed S, Awasthi A, Stahlberg M, Rajput A, Magruder D, et al. A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes. J Gen Physiol. 2017;149:149-170 pubmed publisher
immunocytochemistry; rat; 1 ug/ml; fig 2c	Kawahara K, Hirata H, Ohbuchi K, Nishi K, Maeda A, Kuniyasu A, et al. The novel monoclonal antibody 9F5 reveals expression of a fragment of GPNMB/osteoactivin processed by furin-like protease(s) in a subpopulation of microglia in neonatal rat brain. Glia. 2016;64:1938-61 pubmed publisher
immunocytochemistry; human; 1:100; fig 2	Dobrinskikh E, Lewis L, Brian Doctor R, Okamura K, Lee M, Altmann C, et al. Shank2 Regulates Renal Albumin Endocytosis. Physiol Rep. 2015;3: pubmed publisher
western blot; mouse; 1:3000; fig 2	Kaushik S, Cuervo A. Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis. Nat Cell Biol. 2015;17:759-70 pubmed publisher
immunocytochemistry; mouse	Underhill S, Wheeler D, Amara S. Differential regulation of two isoforms of the glial glutamate transporter EAAT2 by DLG1 and CaMKII. J Neurosci. 2015;35:5260-70 pubmed publisher
immunocytochemistry; rat; loading ...; fig 5a	Widagdo J, Chai Y, Ridder M, Chau Y, Johnson R, Sah P, et al. Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation. Cell Rep. 2015;10:783-795 pubmed publisher
western blot; mouse; 1:1000	Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
	Huang T, Zhao Y, Li X, Wang X, Tseng I, Thompson R, et al. SNX27 and SORLA Interact to Reduce Amyloidogenic Subcellular Distribution and Processing of Amyloid Precursor Protein. J Neurosci. 2016;36:7996-8011 pubmed publisher
	Hou Y, Ernst S, Stuenkel E, Lentz S, Williams J. Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion. PLoS ONE. 2015;10:e0125596 pubmed publisher
	Rong Y, Liu M, Ma L, Du W, Zhang H, Tian Y, et al. Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation. Nat Cell Biol. 2012;14:924-34 pubmed publisher
	Thomas D, Martin C, Weng N, Byrne J, Groblewski G. Tumor protein D52 expression and Ca2+-dependent phosphorylation modulates lysosomal membrane protein trafficking to the plasma membrane. Am J Physiol Cell Physiol. 2010;298:C725-39 pubmed publisher
	Grampp T, Notz V, Broll I, Fischer N, Benke D. Constitutive, agonist-accelerated, recycling and lysosomal degradation of GABA(B) receptors in cortical neurons. Mol Cell Neurosci. 2008;39:628-37 pubmed publisher
	Daniele T, Di Tullio G, Santoro M, Turacchio G, De Matteis M. ARAP1 regulates EGF receptor trafficking and signalling. Traffic. 2008;9:2221-35 pubmed publisher
	Wreden C, Johnson J, Tran C, Seal R, Copenhagen D, Reimer R, et al. The H+-coupled electrogenic lysosomal amino acid transporter LYAAT1 localizes to the axon and plasma membrane of hippocampal neurons. J Neurosci. 2003;23:1265-75 pubmed
	Sans N, Racca C, Petralia R, Wang Y, McCallum J, Wenthold R. Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway. J Neurosci. 2001;21:7506-16 pubmed
	Isobe I, Yanagisawa K, Michikawa M. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) causes Akt phosphorylation and morphological changes in intracellular organellae in cultured rat astrocytes. J Neurochem. 2001;77:274-80 pubmed
	Lewis V, Green S, Marsh M, Vihko P, Helenius A, Mellman I. Glycoproteins of the lysosomal membrane. J Cell Biol. 1985;100:1839-47 pubmed
	Harter C, Mellman I. Transport of the lysosomal membrane glycoprotein lgp120 (lgp-A) to lysosomes does not require appearance on the plasma membrane. J Cell Biol. 1992;117:311-25 pubmed

product information

Product ID :

ADI-VAM-EN001

Catalog Number :

ADI-VAM-EN001-D

Product Name :

LAMP1 monoclonal antibody (Ly1C6)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

22885770, 20032513, 18948198, 18764928, 12598615, 11567040, 11279283, 3922993, 1560028

Clone :

LY1C6

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Rat, Hamster

Background :

Lysosome-associated membrane proteins (LAMP1 and LAMP2) are type I integral membrane protein that are transported from the trans-Golgi network to endosomes and then lysosomes. Following activation of platelets, T-cells, neutrophils, or endothelium, LAMP1 and LAMP2 are expressed on the cell surface. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and loss of LAMP2 expression is associated with impaired autophagy observed in Danons disease.

ALTnames :

Lysosomal-associated membrane protein 1

Immunogen :

Rat liver lysosomal membrane preparation.

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~120kDa by Western blot.

Application Summary :

ICC, IF, IP, WB, Electron microscopy

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage