HSP60 (rat), (recombinant) | Enzo Life Sciences ADI-SPP-742-F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

HSP60 (rat), (recombinant)

catalog :

ADI-SPP-742-F

quantity :

200 µg

citations: 2

Reference
Habich C, Kempe K, van der Zee R, Burkart V, Kolb H. Different heat shock protein 60 species share pro-inflammatory activity but not binding sites on macrophages. FEBS Lett. 2003;533:105-9 pubmed
Bethke K, Staib F, Distler M, Schmitt U, Jonuleit H, Enk A, et al. Different efficiency of heat shock proteins (HSP) to activate human monocytes and dendritic cells: superiority of HSP60. J Immunol. 2002;169:6141-8 pubmed

product information

Product ID :

ADI-SPP-742

Catalog Number :

ADI-SPP-742-F

Product Name :

HSP60 (rat), (recombinant)

Product Type :

Protein

Size :

200 µg

list of Pubmed id :

12505167, 12444117

Background :

Rat Hsp60 belongs to a highly conserved family that includes molecular chaperones from a variety of species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E. coli Hsp60, and the 65 kDa major antigen of mycobacteria. Hsp60 is localized in the mitochondrial matrix of eukaryotes, and in the chloroplast of plants. Hsp60s from divergent species share a number of common characteristics: high abundance; induction with environmental stress such as heat shock; homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg+ and ATP; ATPase activity; and a role in folding and assembly of oligomeric protein structures. These similarities correspond with studies in which the single-ring human mitochondrial homolog Hsp60 and its co-chaperonin Hsp10 were expressed in anE. coli strain engineered to keep the groE operon under strict regulatory control. The findings demonstrate that expression of Hsp60 -Hsp10 enabled successful performance of all essential in vivo functions of GroEL and its co-chaperonin, GroES. Several studies reveal a possible link between members of the Hsp60 family and a number of autoimmune diseases, atherosclerosis, and chlamydial dise ase. Overexpression of self Hsp60 is seen in the synovial tissue of rheumatoid arthritic (RA) patients , and can accompany both cellular and humoral reactivity against Hsp60 in RA. Chlamydial heat shock protein Hsp60, a homolog ofE. coli GroEL, appears capable of eliciting macrophage activation, and several studies reveal a correlation between Hsp60 responses and the immunopathologic manifestations of human chlamydial disease.

ALTnames :

Chaperonin 60, CPN60, HspD1, Heat shock protein 60

Purity :

≥85% (SDS-PAGE; Western blot)

Buffer :

Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.1mM PMSF, and 1.0mM DTT.

Uses :

ATPase activity assay (positive). Western blot control.

Application Summary :

WB, in vitro Assay, Activity assay

Storage :

-80°C