DnaK (E. coli), (recombinant) | Enzo Life Sciences ADI-SPP-630-D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

DnaK (E. coli), (recombinant)

catalog :

ADI-SPP-630-D

quantity :

50 µg

citations: 6

Reference
Cassone M, Vogiatzi P, La Montagna R, de Olivier Inacio V, Cudic P, Wade J, et al. Scope and limitations of the designer proline-rich antibacterial peptide dimer, A3-APO, alone or in synergy with conventional antibiotics. Peptides. 2008;29:1878-86 pubmed publisher
Vostiar I, Tkac J, Mandenius C. Monitoring of the heat-shock response in Escherichia coli using an optical biosensor. Anal Biochem. 2003;322:156-63 pubmed
Liu Z, Tek V, Akoev V, Zolkiewski M. Conserved amino acid residues within the amino-terminal domain of ClpB are essential for the chaperone activity. J Mol Biol. 2002;321:111-20 pubmed
Maier J, Haug M, Föll J, Beck H, Kalbacher H, Rammensee H, et al. Possible association of non-binding of HSP70 to HLA-DRB1 peptide sequences and protection from rheumatoid arthritis. Immunogenetics. 2002;54:67-73 pubmed
Vandenbroeck K, Alloza I, Brehmer D, Billiau A, Proost P, McFerran N, et al. The conserved helix C region in the superfamily of interferon-gamma /interleukin-10-related cytokines corresponds to a high-affinity binding site for the HSP70 chaperone DnaK. J Biol Chem. 2002;277:25668-76 pubmed
Agou F, Ye F, Goffinont S, Courtois G, Yamaoka S, Israel A, et al. NEMO trimerizes through its coiled-coil C-terminal domain. J Biol Chem. 2002;277:17464-75 pubmed

product information

Product ID :

ADI-SPP-630

Catalog Number :

ADI-SPP-630-D

Product Name :

DnaK (E. coli), (recombinant)

Product Type :

Protein

Size :

50 µg

list of Pubmed id :

18721837, 14596822, 12139937, 12037598, 11970958, 11877453

Background :

DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.

ALTnames :

Heat shock protein 70, HSP70

Purity :

≥95% (SDS-PAGE; Western blot)

Buffer :

Liquid. In 40mM TRIS, pH 7.5, containing 80mM sodium chloride, 0.8mM DTT, 0.08mM PMSF, and 20% glycerol.

Uses :

ATPase activity assay (positive). Western blot control.

Application Summary :

ELISA, WB, in vitro Assay, Activity assay

Storage :

-80°C