HSF1 monoclonal antibody (10H8) | Enzo Life Sciences ADI-SPA-950-F product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSF1 monoclonal antibody (10H8)

catalog :

ADI-SPA-950-F

quantity :

200 µg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

10H8

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; fig 1g western blot; human	Gómez Pastor R, Burchfiel E, Neef D, Jaeger A, Cabiscol E, McKinstry S, et al. Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease. Nat Commun. 2017;8:14405 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Gong J, Weng D, Eguchi T, Murshid A, Sherman M, Song B, et al. Targeting the hsp70 gene delays mammary tumor initiation and inhibits tumor cell metastasis. Oncogene. 2015;34:5460-71 pubmed publisher
western blot; rat; 1:500	Moura C, Lollo P, Morato P, Nisishima L, Carneiro E, Amaya Farfan J. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS ONE. 2014;9:e83437 pubmed publisher
western blot; mouse; 1:1000; fig 4	Reina C, Nabet B, Young P, Pittman R. Basal and stress-induced Hsp70 are modulated by ataxin-3. Cell Stress Chaperones. 2012;17:729-42 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Murshid A, Chou S, Prince T, Zhang Y, Bharti A, Calderwood S. Protein kinase A binds and activates heat shock factor 1. PLoS ONE. 2010;5:e13830 pubmed publisher
western blot; mouse; 1:1000	Murapa P, Ward M, Gandhapudi S, Woodward J, D Orazio S. Heat shock factor 1 protects mice from rapid death during Listeria monocytogenes infection by regulating expression of tumor necrosis factor alpha during fever. Infect Immun. 2011;79:177-84 pubmed publisher
	White P, Subramanian C, Zhu Q, Zhang H, Zhao H, Gallagher R, et al. Novel HSP90 inhibitors effectively target functions of thyroid cancer stem cell preventing migration and invasion. Surgery. 2016;159:142-51 pubmed publisher

product information

Product ID :

ADI-SPA-950

Catalog Number :

ADI-SPA-950-F

Product Name :

HSF1 monoclonal antibody (10H8)

Host species :

Rat

Clonality :

Monoclonal

Product Type :

Antibody

Size :

200 µg

list of Pubmed id :

26542767, 22777893

Clone :

10H8

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Rabbit, Monkey

Background :

HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

ALTnames :

HSTF1, Heat shock factor protein 1

Immunogen :

Recombinant mouse HSF1.

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Predicted MW of ~60kDa. Detects a band of ~85kDa by Western blot.

Application Summary :

IHC (PS), WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage