HO-1 polyclonal antibody | Enzo Life Sciences ADI-SPA-896-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HO-1 polyclonal antibody

catalog :

ADI-SPA-896-D

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig s1a	Chen C, Wang S, Yu L, Mueller J, Fortunato F, Rausch V, et al. H2O2-mediated autophagy during ethanol metabolism. Redox Biol. 2021;46:102081 pubmed publisher
immunocytochemistry; human; loading ...; fig 5b	Krzeptowski W, Chudy P, Sokołowski G, Zukowska M, Kusienicka A, Seretny A, et al. Proximity Ligation Assay Detection of Protein-DNA Interactions-Is There a Link between Heme Oxygenase-1 and G-quadruplexes?. Antioxidants (Basel). 2021;10: pubmed publisher
western blot; human; 1:2000; loading ...; fig 2a	Detsika M, Myrtsi E, Koulocheri S, Haroutounian S, Lianos E, Roussos C. Induction of decay accelerating factor and membrane cofactor protein by resveratrol attenuates complement deposition in human coronary artery endothelial cells. Biochem Biophys Rep. 2019;19:100652 pubmed publisher
western blot; mouse; loading ...; fig 1b	Inouye S, Hatori Y, Kubo T, Saito S, Kitamura H, Akagi R. NRF2 and HSF1 coordinately regulate heme oxygenase-1 expression. Biochem Biophys Res Commun. 2018;506:7-11 pubmed publisher
	Scharn C, Collins A, Nair V, Stamm C, MARCIANO D, Graviss E, et al. Heme Oxygenase-1 Regulates Inflammation and Mycobacterial Survival in Human Macrophages during Mycobacterium tuberculosis Infection. J Immunol. 2016;196:4641-9 pubmed publisher
	Oh S, Hwang J, Lee Y, Choi S, Kim J, Kim J, et al. Isolation of basal membrane proteins from BeWo cells and their expression in placentas from fetal growth-restricted pregnancies. Placenta. 2016;39:24-32 pubmed publisher
	Marques L, Negre Salvayre A, Costa L, Canonne Hergaux F. Iron gene expression profile in atherogenic Mox macrophages. Biochim Biophys Acta. 2016;1862:1137-46 pubmed publisher
	van den Bosch T, Boichenko A, Leus N, Ourailidou M, Wapenaar H, Rotili D, et al. The histone acetyltransferase p300 inhibitor C646 reduces pro-inflammatory gene expression and inhibits histone deacetylases. Biochem Pharmacol. 2016;102:130-140 pubmed publisher
	Mercau M, Repetto E, Perez M, Martinez Calejman C, Sánchez Puch S, Finkielstein C, et al. Moderate Exercise Prevents Functional Remodeling of the Anterior Pituitary Gland in Diet-Induced Insulin Resistance in Rats: Role of Oxidative Stress and Autophagy. Endocrinology. 2016;157:1135-45 pubmed publisher
	Mohammed B, Fisher B, Kraskauskas D, Ward S, Wayne J, Brophy D, et al. Vitamin C promotes wound healing through novel pleiotropic mechanisms. Int Wound J. 2016;13:572-84 pubmed publisher

product information

Product ID :

ADI-SPA-896

Catalog Number :

ADI-SPA-896-D

Product Name :

HO-1 polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

26718586, 26672805, 26330549, 26165190, 25753484, 21697243, 19617415, 18846504, 12588181, 12395150

SPECIES REACTIVITY :

Human, Mouse, Rat, Rabbit, Monkey, Hamster, Dog

Background :

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

ALTnames :

HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1

Immunogen :

Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1.

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~32kDa by Western blot.

Application Summary :

IHC (PS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage