HO-1 polyclonal antibody | Enzo Life Sciences ADI-SPA-895-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HO-1 polyclonal antibody

catalog :

ADI-SPA-895-D

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:2000; loading ...; fig 2a	Ghosh A, Abdo S, Zhao S, Wu C, Shi Y, Lo C, et al. Insulin Inhibits Nrf2 Gene Expression via Heterogeneous Nuclear Ribonucleoprotein F/K in Diabetic Mice. Endocrinology. 2017;158:903-919 pubmed publisher
western blot; human; 1:1000; fig 3	Pan H, Guan D, Liu X, Li J, Wang L, Wu J, et al. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Res. 2016;26:190-205 pubmed publisher
	Chen Roetling J, Regan R. Haptoglobin increases the vulnerability of CD163-expressing neurons to hemoglobin. J Neurochem. 2016;139:586-595 pubmed publisher
	Halin Bergström S, Nilsson M, Adamo H, Thysell E, Jernberg E, Stattin P, et al. Extratumoral Heme Oxygenase-1 (HO-1) Expressing Macrophages Likely Promote Primary and Metastatic Prostate Tumor Growth. PLoS ONE. 2016;11:e0157280 pubmed publisher
	Cao S, Zheng M, Hua Y, Chen G, Keep R, Xi G. Hematoma Changes During Clot Resolution After Experimental Intracerebral Hemorrhage. Stroke. 2016;47:1626-31 pubmed publisher
	Kim S, Choi K, Cho S, Yun S, Jeon J, Koh Y, et al. Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors. Sci Rep. 2016;6:24933 pubmed publisher
	Deliyanti D, Lee J, Petratos S, Meyer C, Ward K, Wilkinson Berka J, et al. A potent Nrf2 activator, dh404, bolsters antioxidant capacity in glial cells and attenuates ischaemic retinopathy. Clin Sci (Lond). 2016;130:1375-87 pubmed publisher
	Chen K, Huang K, Lin C, Weng W, Hsu L, Goto S, et al. MicroRNA-27b Enhances the Hepatic Regenerative Properties of Adipose-Derived Mesenchymal Stem Cells. Mol Ther Nucleic Acids. 2016;5:e285 pubmed publisher
	Fujie T, Murakami M, Yoshida E, Tachinami T, Shinkai Y, Fujiwara Y, et al. Copper diethyldithiocarbamate as an activator of Nrf2 in cultured vascular endothelial cells. J Biol Inorg Chem. 2016;21:263-73 pubmed publisher
	Kongpetch S, Puapairoj A, Ong C, Senggunprai L, Prawan A, Kukongviriyapan U, et al. Haem oxygenase 1 expression is associated with prognosis in cholangiocarcinoma patients and with drug sensitivity in xenografted mice. Cell Prolif. 2016;49:90-101 pubmed publisher
	Cheng T, Wang W, Li Q, Han X, Xing J, Qi C, et al. Cerebroprotection of flavanol (-)-epicatechin after traumatic brain injury via Nrf2-dependent and -independent pathways. Free Radic Biol Med. 2016;92:15-28 pubmed publisher
	Barone E, Di Domenico F, Cassano T, Arena A, Tramutola A, Lavecchia M, et al. Impairment of biliverdin reductase-A promotes brain insulin resistance in Alzheimer disease: A new paradigm. Free Radic Biol Med. 2016;91:127-42 pubmed publisher
	Lim J, van der Pol S, Di Dio F, Van het Hof B, Kooij G, de Vries H, et al. Protective effects of monomethyl fumarate at the inflamed blood-brain barrier. Microvasc Res. 2016;105:61-9 pubmed publisher
	Kang L, Grande J, Hillestad M, Croatt A, Barry M, Katusic Z, et al. A new model of an arteriovenous fistula in chronic kidney disease in the mouse: beneficial effects of upregulated heme oxygenase-1. Am J Physiol Renal Physiol. 2016;310:F466-76 pubmed publisher

product information

Product ID :

ADI-SPA-895

Catalog Number :

ADI-SPA-895-D

Product Name :

HO-1 polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

26724590, 26698666, 26679389, 26296046, 26238177, 26237274, 26071936, 25667042, 25148934, 25054000

Image Legend 4 :

Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10µg/ml.

SPECIES REACTIVITY :

Human, Mouse, Rat, Sheep, Rabbit, Dog

Background :

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

ALTnames :

HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1

Immunogen :

Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region.

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~32kDa by Western blot.

Application Summary :

FC, ICC, IHC (PS), WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage