PDI monoclonal antibody (1D3) (DyLight™ 488 conjugate) | Enzo Life Sciences ADI-SPA-891-488-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

PDI monoclonal antibody (1D3) (DyLight™ 488 conjugate)

catalog :

ADI-SPA-891-488-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

DyLight488

clone name :

1D3

reactivity :

human, mouse, rat, dogs, chicken

application :

western blot, flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:5000; fig 1.a,b	Treacy Abarca S, Mukherjee S. Legionella suppresses the host unfolded protein response via multiple mechanisms. Nat Commun. 2015;6:7887 pubmed publisher
western blot; mouse	McAlpine C, Bowes A, Khan M, Shi Y, Werstuck G. Endoplasmic reticulum stress and glycogen synthase kinase-3? activation in apolipoprotein E-deficient mouse models of accelerated atherosclerosis. Arterioscler Thromb Vasc Biol. 2012;32:82-91 pubmed publisher
	Bolar N, Golzio C, Zivná M, Hayot G, Van Hemelrijk C, Schepers D, et al. Heterozygous Loss-of-Function SEC61A1 Mutations Cause Autosomal-Dominant Tubulo-Interstitial and Glomerulocystic Kidney Disease with Anemia. Am J Hum Genet. 2016;99:174-87 pubmed publisher
	MÃ¤tlik K, Yu L, Eesmaa A, Hellman M, Lindholm P, PerÃ¤nen J, et al. Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity. Cell Death Dis. 2015;6:e2032 pubmed publisher
	Capuano A, Bucciotti F, Farwell K, Tippin Davis B, Mroske C, Hulick P, et al. Diagnostic Exome Sequencing Identifies a Novel Gene, EMILIN1, Associated with Autosomal-Dominant Hereditary Connective Tissue Disease. Hum Mutat. 2016;37:84-97 pubmed publisher
	Grigaravicius P, Kaminska E, HÃ¼bner C, McKinnon P, von Deimling A, Frappart P. Rint1 inactivation triggers genomic instability, ER stress and autophagy inhibition in the brain. Cell Death Differ. 2016;23:454-68 pubmed publisher
	Kelly J, Shao Q, Jagger D, Laird D. Cx30 exhibits unique characteristics including a long half-life when assembled into gap junctions. J Cell Sci. 2015;128:3947-60 pubmed publisher
	Radoshevich L, Impens F, Ribet D, Quereda J, Nam Tham T, Nahori M, et al. ISG15 counteracts Listeria monocytogenes infection. elife. 2015;4: pubmed publisher
	Kato S, Chen J, Cornog K, Zhang H, Roberts J. The Golgi apparatus regulates cGMP-dependent protein kinase I compartmentation and proteolysis. Am J Physiol Cell Physiol. 2015;308:C944-58 pubmed publisher
	Hyun S, Maruri Avidal L, Moss B. Topology of Endoplasmic Reticulum-Associated Cellular and Viral Proteins Determined with Split-GFP. Traffic. 2015;16:787-95 pubmed publisher
	Langer F, Spath B, Fischer C, Stolz M, Ayuk F, Kroger N, et al. Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase. Blood. 2013;121:2324-35 pubmed publisher
	Stantchev T, Paciga M, Lankford C, Schwartzkopff F, Broder C, Clouse K. Cell-type specific requirements for thiol/disulfide exchange during HIV-1 entry and infection. Retrovirology. 2012;9:97 pubmed publisher
	Offerdahl D, Dorward D, Hansen B, Bloom M. A three-dimensional comparison of tick-borne flavivirus infection in mammalian and tick cell lines. PLoS ONE. 2012;7:e47912 pubmed publisher
	Takayanagi S, Fukuda R, Takeuchi Y, Tsukada S, Yoshida K. Gene regulatory network of unfolded protein response genes in endoplasmic reticulum stress. Cell Stress Chaperones. 2013;18:11-23 pubmed publisher

product information

Product ID :

ADI-SPA-891-488

Catalog Number :

ADI-SPA-891-488-D

Product Name :

PDI monoclonal antibody (1D3) (DyLight™ 488 conjugate)

Host species :

Mouse

Clonality :

Monoclonal

Conjugation :

DyLight™ 488

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

23315166, 23206338, 23112871

Clone :

1D3

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Xenopus, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Dog, Chicken, Bovine

Background :

The mammalian protein disulphide-isomerase (PDI) family encompasses several highly divergent proteins involved in the processing and maturation of secretory proteins in the ER by catalyzing the rearrangement of disulphide bonds. PDI, an abundant protein of the ER (>400uM), contains a carboxy-terminal retention signal sequence, KDEL, similar to that of BiP and Grp94. The PDI proteins are characterized by the presence of one or more domains of ~95-110 amino acids related to the cytoplasmic protein thioredoxin. All but the PDI-D subfamily are composed entirely of repeats of such domains, with at least one domain containing - and one domain lacking - a redox-active-Cys-X-X-Cys-tetrapeptide. In addition to roles as redox catalysts and isomerases, PDI proteins perform such functions as peptide binding and cell adhesion, and may conduct chaperone activities. Platelet surface thiols and disulphides play an important role in platelet responses. Catalytically active PDI resides on platelet surfaces where it mediates platelet aggregation and secretion by reducing disulfide bonds, thus exposing fibrinogen receptors in platelets.

ALTnames :

P4HB, protein disulfide isomerase

Immunogen :

Synthetic peptide corresponding to the sequence near the C-terminus of rat PDI.

Buffer :

Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.

Uses :

Detects a band of ~58kDa by Western blot. Cited applications include immunofluorescence.

Application Summary :

Storage :

+4°C

more info or order :

Enzo Life Sciences product webpage