DnaK (E. coli) monoclonal antibody (8E2/2) | Enzo Life Sciences ADI-SPA-880-J product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

DnaK (E. coli) monoclonal antibody (8E2/2)

catalog :

ADI-SPA-880-J

quantity :

1 mg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8E2/2

reactivity :

mouse,

application :

western blot

more info or order :

Enzo Life Sciences product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 5a	Günster R, Matthews S, Holden D, Thurston T. SseK1 and SseK3 Type III Secretion System Effectors Inhibit NF-κB Signaling and Necroptotic Cell Death in Salmonella-Infected Macrophages. Infect Immun. 2017;85: pubmed publisher
	Johnson R, Byrne A, Berger C, Klemm E, Crepin V, Dougan G, et al. The Type III Secretion System Effector SptP of Salmonella enterica Serovar Typhi. J Bacteriol. 2017;199: pubmed publisher
	Lange M, Beck B, Brown J, Farmer B, Barnett L, Webster C. Missing the target: DNAk is a dominant epitope in the humoral immune response of channel catfish (Ictalurus punctatus) to Flavobacterium columnare. Fish Shellfish Immunol. 2016;51:170-179 pubmed publisher
	White P, Subramanian C, Zhu Q, Zhang H, Zhao H, Gallagher R, et al. Novel HSP90 inhibitors effectively target functions of thyroid cancer stem cell preventing migration and invasion. Surgery. 2016;159:142-51 pubmed publisher
	Hu L, Zhang W, Zhou M, Han Q, Gao X, Zeng H, et al. Analysis of Salmonella PhoP/PhoQ regulation by dimethyl-SRM-based quantitative proteomics. Biochim Biophys Acta. 2016;1864:20-8 pubmed publisher
	Zhang Y, Tam J, Mena P, van der Velden A, Bliska J. CCR2+ Inflammatory Dendritic Cells and Translocation of Antigen by Type III Secretion Are Required for the Exceptionally Large CD8+ T Cell Response to the Protective YopE69-77 Epitope during Yersinia Infection. PLoS Pathog. 2015;11:e1005167 pubmed publisher
	Unterweger D, Kostiuk B, Ã–tjengerdes R, Wilton A, Diaz Satizabal L, Pukatzki S. Chimeric adaptor proteins translocate diverse type VI secretion system effectors in Vibrio cholerae. EMBO J. 2015;34:2198-210 pubmed publisher
	Zininga T, Achilonu I, Hoppe H, Prinsloo E, Dirr H, Shonhai A. Overexpression, Purification and Characterisation of the Plasmodium falciparum Hsp70-z (PfHsp70-z) Protein. PLoS ONE. 2015;10:e0129445 pubmed publisher
	Flores Kim J, Darwin A. Activity of a bacterial cell envelope stress response is controlled by the interaction of a protein binding domain with different partners. J Biol Chem. 2015;290:11417-30 pubmed publisher
	Garza Mayers A, Miller K, Russo B, Nagda D, Goldberg M. Shigella flexneri regulation of ARF6 activation during bacterial entry via an IpgD-mediated positive feedback loop. MBio. 2015;6:e02584 pubmed publisher
	van Lier C, Tiner B, Chauhan S, Motin V, Fitts E, Huante M, et al. Further characterization of a highly attenuated Yersinia pestis CO92 mutant deleted for the genes encoding Braun lipoprotein and plasminogen activator protease in murine alveolar and primary human macrophages. Microb Pathog. 2015;80:27-38 pubmed publisher
	Anderson D, Sato H, Dirck A, Feix J, Frank D. Ubiquitin activates patatin-like phospholipases from multiple bacterial species. J Bacteriol. 2015;197:529-41 pubmed publisher
	Cassone M, Vogiatzi P, La Montagna R, de Olivier Inacio V, Cudic P, Wade J, et al. Scope and limitations of the designer proline-rich antibacterial peptide dimer, A3-APO, alone or in synergy with conventional antibiotics. Peptides. 2008;29:1878-86 pubmed publisher
	Ashtekar A, Zhang P, Katz J, Deivanayagam C, Rallabhandi P, Vogel S, et al. TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis. J Leukoc Biol. 2008;84:1434-46 pubmed publisher
	Fredriksson A, Ballesteros M, Peterson C, Persson O, Silhavy T, Nystrom T. Decline in ribosomal fidelity contributes to the accumulation and stabilization of the master stress response regulator sigmaS upon carbon starvation. Genes Dev. 2007;21:862-74 pubmed
	Perez Rodriguez R, Fisher A, Perlmutter J, Hicks M, Chanal A, Santini C, et al. An essential role for the DnaK molecular chaperone in stabilizing over-expressed substrate proteins of the bacterial twin-arginine translocation pathway. J Mol Biol. 2007;367:715-30 pubmed

product information

Product ID :

ADI-SPA-880

Catalog Number :

ADI-SPA-880-J

Product Name :

DnaK (E. coli) monoclonal antibody (8E2/2)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

1 mg

list of Pubmed id :

26542767, 26468944, 26083397, 25802329, 25736891, 25697665, 18721837, 18708593, 17403784, 17280684

Clone :

8E2/2

Isotype :

IgG1

SPECIES REACTIVITY :

E. coli

Background :

DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.

ALTnames :

Hsp70

Immunogen :

E. coli DnaK.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~70kDa by Western blot.

Application Summary :

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage