product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Grp94 monoclonal antibody (9G10)
catalog :
ADI-SPA-850-D
quantity :
50 µg
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
9G10

The same clone is also sold as:
reactivity :
human, mouse, rat, dogs, chicken, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry knockout validation
more info or order :
Enzo Life Sciences product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; fig 2
Rachidi S, Sun S, Wu B, Jones E, Drake R, Ogretmen B, et al. Endoplasmic reticulum heat shock protein gp96 maintains liver homeostasis and promotes hepatocellular carcinogenesis. J Hepatol. 2015;62:879-88 pubmed publisher
  • western blot; mouse; loading ...; fig 3c
Fu Y, Zhang B, Mu T. LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochem Biophys Res Commun. 2019;511:356-362 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
Gómez Fernández P, Urtasun A, Paton A, Paton J, Borrego F, Dersh D, et al. Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response. Front Immunol. 2018;9:2934 pubmed publisher
  • ELISA; mouse; loading ...; fig s4c
Keklikoglou I, Cianciaruso C, Güç E, Squadrito M, Spring L, Tazzyman S, et al. Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. Nat Cell Biol. 2019;21:190-202 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2b
Carpier J, Zucchetti A, Bataille L, Dogniaux S, Shafaq Zadah M, Bardin S, et al. Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. J Exp Med. 2018;215:1245-1265 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
Alloatti A, Rookhuizen D, Joannas L, Carpier J, Iborra S, Magalhaes J, et al. Critical role for Sec22b-dependent antigen cross-presentation in antitumor immunity. J Exp Med. 2017;214:2231-2241 pubmed publisher
  • immunohistochemistry; mouse; fig 2
Yamanaka T, Tosaki A, Miyazaki H, Kurosawa M, Koike M, Uchiyama Y, et al. Differential roles of NF-Y transcription factor in ER chaperone expression and neuronal maintenance in the CNS. Sci Rep. 2016;6:34575 pubmed publisher
  • western blot; human; fig s3
Kowal J, Arras G, Colombo M, Jouve M, Morath J, Primdal Bengtson B, et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016;113:E968-77 pubmed publisher
  • western blot; human; fig 2
Chhabra S, Jain S, Wallace C, Hong F, Liu B. High expression of endoplasmic reticulum chaperone grp94 is a novel molecular hallmark of malignant plasma cells in multiple myeloma. J Hematol Oncol. 2015;8:77 pubmed publisher
  • western blot; human; 1:1000; fig 2
Alkhateeb A, Buckett P, Gardeck A, Kim J, Byrne S, Fraenkel P, et al. The small molecule ferristatin II induces hepatic hepcidin expression in vivo and in vitro. Am J Physiol Gastrointest Liver Physiol. 2015;308:G1019-26 pubmed publisher
  • flow cytometry; mouse; fig 7
Zhang Y, Wu B, Metelli A, Thaxton J, Hong F, Rachidi S, et al. GP96 is a GARP chaperone and controls regulatory T cell functions. J Clin Invest. 2015;125:859-69 pubmed publisher
  • western blot; mouse; 1:15,000
Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
  • western blot; mouse
Hong F, Liu B, Chiosis G, Gewirth D, Li Z. ?7 helix region of ?I domain is crucial for integrin binding to endoplasmic reticulum chaperone gp96: a potential therapeutic target for cancer metastasis. J Biol Chem. 2013;288:18243-8 pubmed publisher
  • western blot; mouse
Barton E, Park S, James J, Makarewich C, Philippou A, Eletto D, et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J. 2012;26:3691-702 pubmed publisher
  • flow cytometry; mouse
Wu S, Hong F, Gewirth D, Guo B, Liu B, Li Z. The molecular chaperone gp96/GRP94 interacts with Toll-like receptors and integrins via its C-terminal hydrophobic domain. J Biol Chem. 2012;287:6735-42 pubmed publisher
Dersh D, Iwamoto Y, Argon Y. Tay-Sachs disease mutations in HEXA target the ? chain of hexosaminidase A to endoplasmic reticulum-associated degradation. Mol Biol Cell. 2016;27:3813-3827 pubmed
Di X, Wang Y, Han D, Fu Y, Duerfeldt A, Blagg B, et al. Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation. J Biol Chem. 2016;291:9526-39 pubmed publisher
Collins T, Ylanko J, Geng F, Andrews D. A Versatile Cell Death Screening Assay Using Dye-Stained Cells and Multivariate Image Analysis. Assay Drug Dev Technol. 2015;13:547-57 pubmed publisher
Wang H, Wang X, Ke Z, Comer A, Xu M, Frank J, et al. Tunicamycin-induced unfolded protein response in the developing mouse brain. Toxicol Appl Pharmacol. 2015;283:157-67 pubmed publisher
Panter M, Jain A, Leonhardt R, Ha T, Cresswell P. Dynamics of major histocompatibility complex class I association with the human peptide-loading complex. J Biol Chem. 2012;287:31172-84 pubmed publisher
Xargay Torrent S, Lopez Guerra M, Saborit Villarroya I, Rosich L, Campo E, Roué G, et al. Vorinostat-induced apoptosis in mantle cell lymphoma is mediated by acetylation of proapoptotic BH3-only gene promoters. Clin Cancer Res. 2011;17:3956-68 pubmed publisher
Yang Y, Liu B, Dai J, Srivastava P, Zammit D, Lefrancois L, et al. Heat shock protein gp96 is a master chaperone for toll-like receptors and is important in the innate function of macrophages. Immunity. 2007;26:215-26 pubmed
Berro R, de La Fuente C, Klase Z, Kehn K, Parvin L, Pumfery A, et al. Identifying the membrane proteome of HIV-1 latently infected cells. J Biol Chem. 2007;282:8207-18 pubmed
Bando Y, Katayama T, Kasai K, Taniguchi M, Tamatani M, Tohyama M. GRP94 (94 kDa glucose-regulated protein) suppresses ischemic neuronal cell death against ischemia/reperfusion injury. Eur J Neurosci. 2003;18:829-40 pubmed
Beckhelling C, Chang P, Chevalier S, Ford C, Houliston E. Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts. Mol Biol Cell. 2003;14:1125-37 pubmed
Yun S, Gartner U, Arendt T, Hoyer S. Increase in vulnerability of middle-aged rat brain to lead by cerebral energy depletion. Brain Res Bull. 2000;52:371-8 pubmed
Allen S, Abuzenadah A, Hinks J, Blagg J, Gursel T, Ingerslev J, et al. A novel von Willebrand disease-causing mutation (Arg273Trp) in the von Willebrand factor propeptide that results in defective multimerization and secretion. Blood. 2000;96:560-8 pubmed
Heike M, Frenzel C, Meier D, Galle P. Expression of stress protein gp96, a tumor rejection antigen, in human colorectal cancer. Int J Cancer. 2000;86:489-93 pubmed
product information
Product ID :
ADI-SPA-850
Catalog Number :
ADI-SPA-850-D
Product Name :
Grp94 monoclonal antibody (9G10)
Host species :
Rat
Clonality :
Monoclonal
Product Type :
Antibody
Size :
50 µg
list of Pubmed id :
26422066, 25620058, 25607841, 17275357, 17237230, 12925009, 12631728, 10922516, 10887119, 10797260
Clone :
9G10
Isotype :
IgG2a
SPECIES REACTIVITY :
Human, Mouse, Rat, Xenopus, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Dog, Chicken, Bovine
Background :
Grp94 (Glucose-regulated protein 94) is an abundant resident endoplasmic reticulum (ER) lumenal stress protein, which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. Grp94 expression is upregulated by stress conditions such as glucose starvation and heat shock, which promote protein misfolding or unfolding. In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.
ALTnames :
Endoplasmin, Tra1, Hsp90B1, Gp96
Immunogen :
Native chicken Grp94.
Buffer :
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~98kDa by Western blot.
Application Summary :
ICC, IF, IHC (PS), IP, WB, Electron microscopy
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.