HSP90 monoclonal antibody (2D12) | Enzo Life Sciences ADI-SPA-845-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSP90 monoclonal antibody (2D12)

catalog :

ADI-SPA-845-D

quantity :

50 µl

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

2D12

The same clone is also sold as:

Novus Biologicals: NBP1-97450-0.05ml

reactivity :

human, mouse, rat, dogs, chicken, bovine

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2	Bzowska M, Nogieć A, Bania K, Zygmunt M, Zarebski M, Dobrucki J, et al. Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages. J Leukoc Biol. 2017;102:763-774 pubmed publisher
immunoprecipitation; human; loading ...; fig 2c western blot; human; loading ...; fig 2a	Chen C, Zhuang Y, Chen X, Chen X, Li D, Fan Y, et al. Hsp90 N- and C-terminal double inhibition synergistically suppresses Bcr-Abl-positive human leukemia cells. Oncotarget. 2017;8:10025-10036 pubmed publisher
immunoprecipitation; human; fig 3	Huang K, Chen Z, Jiang Y, Akare S, Kolber Simonds D, Condon K, et al. Apratoxin A Shows Novel Pancreas-Targeting Activity through the Binding of Sec 61. Mol Cancer Ther. 2016;15:1208-16 pubmed publisher
immunoprecipitation; mouse; fig 7 western blot; mouse; fig 5 immunoprecipitation; human; fig 2 western blot; human; fig 10	Saito K, Kukita K, Kutomi G, Okuya K, Asanuma H, Tabeya T, et al. Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE. Eur J Immunol. 2015;45:2028-41 pubmed publisher
western blot; human	Sarkar S, Brautigan D, Parsons S, Larner J. Androgen receptor degradation by the E3 ligase CHIP modulates mitotic arrest in prostate cancer cells. Oncogene. 2014;33:26-33 pubmed publisher
	Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
	Chen Y, Schnetz M, Irarrazabal C, Shen R, Williams C, Burg M, et al. Proteomic identification of proteins associated with the osmoregulatory transcription factor TonEBP/OREBP: functional effects of Hsp90 and PARP-1. Am J Physiol Renal Physiol. 2007;292:F981-92 pubmed
	Russell K, Haynes M, Caulin Glaser T, Rosneck J, Sessa W, Bender J. Estrogen stimulates heat shock protein 90 binding to endothelial nitric oxide synthase in human vascular endothelial cells. Effects on calcium sensitivity and NO release. J Biol Chem. 2000;275:5026-30 pubmed
	Brunt S, Perdew G, Toft D, Silver J. Hsp90-containing multiprotein complexes in the eukaryotic microbe Achlya. Cell Stress Chaperones. 1998;3:44-56 pubmed
	Garcia Cardena G, Fan R, Shah V, Sorrentino R, Cirino G, Papapetropoulos A, et al. Dynamic activation of endothelial nitric oxide synthase by Hsp90. Nature. 1998;392:821-4 pubmed

product information

Product ID :

ADI-SPA-845

Catalog Number :

ADI-SPA-845-D

Product Name :

HSP90 monoclonal antibody (2D12)

Host species :

Rat

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µl

list of Pubmed id :

17148781, 10671543, 9585181, 9580552

Clone :

2D12

Isotype :

IgM

SPECIES REACTIVITY :

Human, Mouse, Rat, Water mold, Sheep, Rabbit, Monkey, Hamster, Guinea pig, Fish, Dog, Chicken, Bovine, Beluga

Background :

The 90kDa molecular chaperone family includes 90 kDa heat shock protein Hsp90 and 94 kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90α and Hsp90β, encoded by separate genes. The amino acid sequences of human and yeast Hsp90-alpha are 85% and 90% homologous to those of Hsp90β , respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signaling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophila Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits even after Hsp90 function is restored.

ALTnames :

HSP86, Heat shock protein 90β

Immunogen :

Native mouse Hsp90.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~90kDa by Western blot.

Application Summary :

IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage