HSP90 monoclonal antibody (16F1) | Enzo Life Sciences ADI-SPA-835-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSP90 monoclonal antibody (16F1)

catalog :

ADI-SPA-835-D

quantity :

50 µg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

16F1

The same clone is also sold as:

Novus Biologicals: NBP1-97506-0.05mg

reactivity :

Garra rufa, human, mouse, rat, dogs, chicken, bovine, fruit fly

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - free floating section

more info or order :

Enzo Life Sciences product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2	Bzowska M, Nogieć A, Bania K, Zygmunt M, Zarebski M, Dobrucki J, et al. Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages. J Leukoc Biol. 2017;102:763-774 pubmed publisher
western blot; mouse; loading ...; fig 2	Amlie Wolf A, Ryvkin P, Tong R, Dragomir I, Suh E, Xu Y, et al. Transcriptomic Changes Due to Cytoplasmic TDP-43 Expression Reveal Dysregulation of Histone Transcripts and Nuclear Chromatin. PLoS ONE. 2015;10:e0141836 pubmed publisher
immunohistochemistry - free floating section; fruit fly ; 1:100; fig 3	Pearce M, Spartz E, Hong W, Luo L, Kopito R. Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain. Nat Commun. 2015;6:6768 pubmed publisher
western blot; Garra rufa	Oksala N, EkmekÃ§i F, Ozsoy E, Kirankaya S, Kokkola T, Emecen G, et al. Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress. Redox Biol. 2014;3:25-8 pubmed publisher
western blot; mouse	Niesman I, Schilling J, Shapiro L, Kellerhals S, Bonds J, Kleschevnikov A, et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation. 2014;11:39 pubmed publisher
western blot; human	Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
	Sager R, Woodford M, Shapiro O, Mollapour M, Bratslavsky G. Sporadic renal angiomyolipoma in a patient with Birt-Hogg-Dubé: chaperones in pathogenesis. Oncotarget. 2018;9:22220-22229 pubmed publisher
	Conos S, Lawlor K, Vaux D, Vince J, Lindqvist L. Cell death is not essential for caspase-1-mediated interleukin-1Î² activation and secretion. Cell Death Differ. 2016;23:1827-1838 pubmed publisher
	Woodford M, Dunn D, Blanden A, Capriotti D, Loiselle D, Prodromou C, et al. The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding. Nat Commun. 2016;7:12037 pubmed publisher
	Andreeva N, Zatsepina O, Garbuz D, Evgen ev M, Belyavsky A. Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells. Cell Stress Chaperones. 2016;21:727-33 pubmed publisher
	Kavlashvili T, Jia Y, Dai D, Meng X, Thiel K, Leslie K, et al. Inverse Relationship between Progesterone Receptor and Myc in Endometrial Cancer. PLoS ONE. 2016;11:e0148912 pubmed publisher
	Jacobsen A, Lowes K, Tanzer M, Lucet I, Hildebrand J, Petrie E, et al. HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death. Cell Death Dis. 2016;7:e2051 pubmed publisher
	Clemente Vicario F, Alvarez C, ROWELL J, Roy S, London C, Kisseberth W, et al. Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines. PLoS ONE. 2015;10:e0142007 pubmed publisher
	Narayan M, Seeley K, Jinwal U. Identification and quantitative analysis of cellular proteins affected by treatment with withaferin a using a SILAC-based proteomics approach. J Ethnopharmacol. 2015;175:86-92 pubmed publisher
	Macri C, Wang F, Tasset I, Schall N, Page N, Briand J, et al. Modulation of deregulated chaperone-mediated autophagy by a phosphopeptide. Autophagy. 2015;11:472-86 pubmed publisher
	Nanduri P, Hao R, Fitzpatrick T, Yao T. Chaperone-mediated 26S proteasome remodeling facilitates free K63 ubiquitin chain production and aggresome clearance. J Biol Chem. 2015;290:9455-64 pubmed publisher
	Wu L, Wang C, Boisson B, Misra S, Rayman P, Finke J, et al. The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling. J Immunol. 2014;193:1590-9 pubmed publisher
	Orenstein S, Kuo S, Tasset I, Arias E, Koga H, Fernandez Carasa I, et al. Interplay of LRRK2 with chaperone-mediated autophagy. Nat Neurosci. 2013;16:394-406 pubmed publisher
	Wang C, Wu L, Bulek K, Martin B, Zepp J, Kang Z, et al. The psoriasis-associated D10N variant of the adaptor Act1 with impaired regulation by the molecular chaperone hsp90. Nat Immunol. 2013;14:72-81 pubmed publisher
	Hasham M, Snow K, Donghia N, Branca J, Lessard M, Stavnezer J, et al. Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase. J Immunol. 2012;189:2374-82 pubmed publisher
	Bento C, Marques F, Fernandes R, Pereira P. Methylglyoxal alters the function and stability of critical components of the protein quality control. PLoS ONE. 2010;5:e13007 pubmed publisher
	Sagan D, Mörtl S, Muller I, Eckardt Schupp F, Eichholtz Wirth H. Enhanced CD95-mediated apoptosis contributes to radiation hypersensitivity of NBS lymphoblasts. Apoptosis. 2007;12:753-67 pubmed
	Yamano T, Murata S, Shimbara N, Tanaka N, Chiba T, Tanaka K, et al. Two distinct pathways mediated by PA28 and hsp90 in major histocompatibility complex class I antigen processing. J Exp Med. 2002;196:185-96 pubmed

product information

Product ID :

ADI-SPA-835

Catalog Number :

ADI-SPA-835-D

Product Name :

HSP90 monoclonal antibody (16F1)

Host species :

Rat

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

26560147, 26392330, 25866135, 25024377, 23202271, 22826323, 20885985, 17219051

Clone :

16F1

Isotype :

IgG2a

SPECIES REACTIVITY :

Human, Mouse, Rat, Xenopus, Sheep, Scallop, Rabbit, Plant, Porcine, Opossum, Mussel, Monkey, Hamster, Guinea pig, Fish, Drosophila, Dog, Chicken, Bovine, Beluga

Background :

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

ALTnames :

Heat shock protein 90

Immunogen :

Native human Hsp90.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~90kDa by Western blot.

Application Summary :

IHC (PS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage