product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSP90 monoclonal antibody (AC88)
catalog :
ADI-SPA-830-F
quantity :
200 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AC88

The same clone is also sold as:
reactivity :
human, mouse, rat, dogs, chicken
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:4000; fig 9a
Lüningschrör P, Binotti B, Dombert B, Heimann P, Pérez Lara A, Slotta C, et al. Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease. Nat Commun. 2017;8:678 pubmed publisher
  • flow cytometry; human; loading ...; fig 2
Bzowska M, Nogieć A, Bania K, Zygmunt M, Zarebski M, Dobrucki J, et al. Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages. J Leukoc Biol. 2017;102:763-774 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4d
Adachi E, Sakai K, Nishiuchi T, Imamura R, Sato H, Matsumoto K. Different growth and metastatic phenotypes associated with a cell-intrinsic change of Met in metastatic melanoma. Oncotarget. 2016;7:70779-70793 pubmed publisher
  • western blot; mouse; fig 5
Hogg S, Newbold A, Vervoort S, Cluse L, Martin B, Gregory G, et al. BET Inhibition Induces Apoptosis in Aggressive B-Cell Lymphoma via Epigenetic Regulation of BCL-2 Family Members. Mol Cancer Ther. 2016;15:2030-41 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3
Kruger L, O Malley H, Hull J, Kleeman A, Patino G, Isom L. ?1-C121W Is Down But Not Out: Epilepsy-Associated Scn1b-C121W Results in a Deleterious Gain-of-Function. J Neurosci. 2016;36:6213-24 pubmed publisher
  • western blot; human; fig 1
Tribollet V, Barenton B, Kroiss A, Vincent S, Zhang L, Forcet C, et al. miR-135a Inhibits the Invasion of Cancer Cells via Suppression of ERR?. PLoS ONE. 2016;11:e0156445 pubmed publisher
  • immunoprecipitation; human; fig 3
Huang K, Chen Z, Jiang Y, Akare S, Kolber Simonds D, Condon K, et al. Apratoxin A Shows Novel Pancreas-Targeting Activity through the Binding of Sec 61. Mol Cancer Ther. 2016;15:1208-16 pubmed publisher
  • western blot; human; fig 5
Lee J, Kuo C, Tsai S, Cheng S, Chen S, Chan H, et al. Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells. Front Pharmacol. 2016;7:81 pubmed publisher
  • western blot; human; 1:500; tbl 1
  • western blot; mouse; 1:500; tbl 1
Piedrahita D, Castro Álvarez J, Boudreau R, Villegas Lanau A, Kosik K, Gallego Gómez J, et al. β-Secretase 1's Targeting Reduces Hyperphosphorilated Tau, Implying Autophagy Actors in 3xTg-AD Mice. Front Cell Neurosci. 2015;9:498 pubmed publisher
  • flow cytometry; mouse; fig 1
Mikyšková R, Štěpánek I, Indrová M, Bieblová J, Šímová J, Truxová I, et al. Dendritic cells pulsed with tumor cells killed by high hydrostatic pressure induce strong immune responses and display therapeutic effects both in murine TC-1 and TRAMP-C2 tumors when combined with docetaxel chemotherapy. Int J Oncol. 2016;48:953-64 pubmed publisher
  • flow cytometry; human
Dudek Perić A, Ferreira G, Muchowicz A, Wouters J, Prada N, Martin S, et al. Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin. Cancer Res. 2015;75:1603-14 pubmed publisher
  • western blot; mouse
Gong J, Weng D, Eguchi T, Murshid A, Sherman M, Song B, et al. Targeting the hsp70 gene delays mammary tumor initiation and inhibits tumor cell metastasis. Oncogene. 2015;34:5460-71 pubmed publisher
  • flow cytometry; human; 1:100
Martin S, Dudek Perić A, Maes H, Garg A, Gabrysiak M, Demirsoy S, et al. Concurrent MEK and autophagy inhibition is required to restore cell death associated danger-signalling in Vemurafenib-resistant melanoma cells. Biochem Pharmacol. 2015;93:290-304 pubmed publisher
  • western blot; mouse
Moutaoufik M, El Fatimy R, Nassour H, Gareau C, Lang J, Tanguay R, et al. UVC-induced stress granules in mammalian cells. PLoS ONE. 2014;9:e112742 pubmed publisher
  • western blot; chicken
Blanc S, Ruggiero F, Birot A, Acloque H, Decimo D, Lerat E, et al. Subcellular localization of ENS-1/ERNI in chick embryonic stem cells. PLoS ONE. 2014;9:e92039 pubmed publisher
  • immunoprecipitation; human; fig 6
  • western blot; human; fig 6
Bots M, Verbrugge I, Martin B, Salmon J, Ghisi M, Baker A, et al. Differentiation therapy for the treatment of t(8;21) acute myeloid leukemia using histone deacetylase inhibitors. Blood. 2014;123:1341-52 pubmed publisher
  • western blot; human
Mao R, Rubio V, Chen H, Bai L, Mansour O, Shi Z. OLA1 protects cells in heat shock by stabilizing HSP70. Cell Death Dis. 2013;4:e491 pubmed publisher
  • western blot; human; 1:1000
Takahashi A, Torigoe T, Tamura Y, Kanaseki T, Tsukahara T, Sasaki Y, et al. Heat shock enhances the expression of cytotoxic granule proteins and augments the activities of tumor-associated antigen-specific cytotoxic T lymphocytes. Cell Stress Chaperones. 2012;17:757-63 pubmed publisher
  • western blot; rat
Peng H, Morishima Y, Pratt W, Osawa Y. Modulation of heme/substrate binding cleft of neuronal nitric-oxide synthase (nNOS) regulates binding of Hsp90 and Hsp70 proteins and nNOS ubiquitination. J Biol Chem. 2012;287:1556-65 pubmed publisher
Hogg S, Motorna O, Cluse L, Johanson T, Coughlan H, Raviram R, et al. Targeting histone acetylation dynamics and oncogenic transcription by catalytic P300/CBP inhibition. Mol Cell. 2021;81:2183-2200.e13 pubmed publisher
Yanda M, Liu Q, Cebotaru L. A potential strategy for reducing cysts in autosomal dominant polycystic kidney disease with a CFTR corrector. J Biol Chem. 2018;293:11513-11526 pubmed publisher
Sevin M, Kubovcakova L, Pernet N, Causse S, Vitte F, Villeval J, et al. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. Nat Commun. 2018;9:1431 pubmed publisher
Fedoseienko A, Wieringa H, Wisman G, Duiker E, Reyners A, Hofker M, et al. Nuclear COMMD1 Is Associated with Cisplatin Sensitivity in Ovarian Cancer. PLoS ONE. 2016;11:e0165385 pubmed publisher
Kasza A, Hunya A, Frank Z, Fulop F, Torok Z, Balogh G, et al. Dihydropyridine Derivatives Modulate Heat Shock Responses and have a Neuroprotective Effect in a Transgenic Mouse Model of Alzheimer's Disease. J Alzheimers Dis. 2016;53:557-71 pubmed publisher
Kallifatidis G, Munoz D, Singh R, Salazar N, Hoy J, Lokeshwar B. β-Arrestin-2 Counters CXCR7-Mediated EGFR Transactivation and Proliferation. Mol Cancer Res. 2016;14:493-503 pubmed publisher
Carter E, Gupta N, Ragsdale S. High Affinity Heme Binding to a Heme Regulatory Motif on the Nuclear Receptor Rev-erbβ Leads to Its Degradation and Indirectly Regulates Its Interaction with Nuclear Receptor Corepressor. J Biol Chem. 2016;291:2196-222 pubmed publisher
Baker A, Gregory G, Verbrugge I, Kats L, Hilton J, Vidacs E, et al. The CDK9 Inhibitor Dinaciclib Exerts Potent Apoptotic and Antitumor Effects in Preclinical Models of MLL-Rearranged Acute Myeloid Leukemia. Cancer Res. 2016;76:1158-69 pubmed publisher
Park Y, Lee K, Park S, Kim J, Kang E, Kim S, et al. Potential Prognostic Value of Histone Deacetylase 6 and Acetylated Heat-Shock Protein 90 in Early-Stage Breast Cancer. J Breast Cancer. 2015;18:249-55 pubmed publisher
Matthews G, Mehdipour P, Cluse L, Falkenberg K, Wang E, Roth M, et al. Functional-genetic dissection of HDAC dependencies in mouse lymphoid and myeloid malignancies. Blood. 2015;126:2392-403 pubmed publisher
Watkins R, Evans Molina C, Terrell J, Day K, Guindon L, Restrepo I, et al. Proinsulin and heat shock protein 90 as biomarkers of beta-cell stress in the early period after onset of type 1 diabetes. Transl Res. 2016;168:96-106.e1 pubmed publisher
Saeed H, Shalaby M, Embaby A, Ismael M, Pathan A, Ataya F, et al. The Arabian camel Camelus dromedarius heat shock protein 90α: cDNA cloning, characterization and expression. Int J Biol Macromol. 2015;81:195-204 pubmed publisher
Di Giancamillo A, Rossi R, Pastorelli G, Deponti D, Carollo V, Casamassima D, et al. The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets. J Anim Sci. 2015;93:2849-59 pubmed publisher
Sailland J, Tribollet V, Forcet C, Billon C, Barenton B, Carnesecchi J, et al. Estrogen-related receptor α decreases RHOA stability to induce orientated cell migration. Proc Natl Acad Sci U S A. 2014;111:15108-13 pubmed publisher
Marra M, Lombardi A, Agostinelli E, Giuberti G, Zappavigna S, Tempera G, et al. Bovine serum amine oxidase and spm potentiate docetaxel and interferon-alpha effects in inducing apoptosis on human cancer cells through the generation of oxidative stress. Biochim Biophys Acta. 2008;1783:2269-78 pubmed publisher
Morishima Y, Wang A, Yu Z, Pratt W, Osawa Y, Lieberman A. CHIP deletion reveals functional redundancy of E3 ligases in promoting degradation of both signaling proteins and expanded glutamine proteins. Hum Mol Genet. 2008;17:3942-52 pubmed publisher
Eder K, Leutenegger C, Köhler H, Werner I. Effects of neurotoxic insecticides on heat-shock proteins and cytokine transcription in Chinook salmon (Oncorhynchus tshawytscha). Ecotoxicol Environ Saf. 2009;72:182-190 pubmed publisher
Stahl M, Retzlaff M, Nassal M, Beck J. Chaperone activation of the hepadnaviral reverse transcriptase for template RNA binding is established by the Hsp70 and stimulated by the Hsp90 system. Nucleic Acids Res. 2007;35:6124-36 pubmed
Chen S, Brown I. Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases. Cell Stress Chaperones. 2007;12:51-8 pubmed
product information
Product ID :
ADI-SPA-830
Catalog Number :
ADI-SPA-830-F
Product Name :
HSP90 monoclonal antibody (AC88)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
200 µg
list of Pubmed id :
26472975, 26397425, 25659585, 25529535, 25288732, 23412384, 18848847, 18784277, 18573527, 17441507
Clone :
AC88
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat, Fusobacterium nucleatum, Water mold, Sheep, Scallop, Rabbit, Porcine, Mussel, Monkey, Mycobacterium leprae, Hamster, Guinea pig, Fish, E. coli, Dog, Chicken, C. elegans, Beluga
Background :
The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.
ALTnames :
Heat shock protein 90
Immunogen :
Achlya ambisexualis (water mold) Hsp90.
Buffer :
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~90kDa by Western blot.
Application Summary :
IHC (PS), WB
Storage :
-20°C
more info or order :
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.