product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
KDEL monoclonal antibody (10C3)
catalog :
ADI-SPA-827-J
quantity :
1 mg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10C3
reactivity :
human, mouse, rat, fruit fly
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 40
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:100; loading ...
Otani Y, Ohno N, Cui J, Yamaguchi Y, Baba H. Upregulation of large myelin protein zero leads to Charcot-Marie-Tooth disease-like neuropathy in mice. Commun Biol. 2020;3:121 pubmed publisher
  • western blot; mouse; loading ...; fig 1d
Massa L pez D, Thelen M, Stahl F, Thiel C, Linhorst A, Sylvester M, et al. The lysosomal transporter MFSD1 is essential for liver homeostasis and critically depends on its accessory subunit GLMP. elife. 2019;8: pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 2c
Siekierska A, Stamberger H, Deconinck T, Oprescu S, Partoens M, Zhang Y, et al. Biallelic VARS variants cause developmental encephalopathy with microcephaly that is recapitulated in vars knockout zebrafish. Nat Commun. 2019;10:708 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 4i
Cabron A, El Azzouzi K, Boss M, Arnold P, Schwarz J, Rosas M, et al. Structural and Functional Analyses of the Shedding Protease ADAM17 in HoxB8-Immortalized Macrophages and Dendritic-like Cells. J Immunol. 2018;201:3106-3118 pubmed publisher
  • immunocytochemistry; fruit fly ; 1:200; loading ...; fig 3d
  • western blot; fruit fly ; 1:2000; loading ...; fig 1b
Umegawachi T, Yoshida H, Koshida H, Yamada M, Ohkawa Y, Sato T, et al. Control of tissue size and development by a regulatory element in the yorkie 3'UTR. Am J Cancer Res. 2017;7:673-687 pubmed
  • western blot; human; loading ...; fig 8a
Nakajima H, Itakura M, Kubo T, Kaneshige A, Harada N, Izawa T, et al. Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Aggregation Causes Mitochondrial Dysfunction during Oxidative Stress-induced Cell Death. J Biol Chem. 2017;292:4727-4742 pubmed publisher
  • western blot; rat; loading ...; fig 5a
Cóppola Segovia V, Cavarsan C, Maia F, Ferraz A, Nakao L, Lima M, et al. ER Stress Induced by Tunicamycin Triggers ?-Synuclein Oligomerization, Dopaminergic Neurons Death and Locomotor Impairment: a New Model of Parkinson's Disease. Mol Neurobiol. 2017;54:5798-5806 pubmed publisher
  • immunocytochemistry; human; fig 2
Reisen F, Sauty De Chalon A, Pfeifer M, Zhang X, Gabriel D, Selzer P. Linking phenotypes and modes of action through high-content screen fingerprints. Assay Drug Dev Technol. 2015;13:415-27 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig s5
Zhang Y, Jansen West K, Xu Y, Gendron T, Bieniek K, Lin W, et al. Aggregation-prone c9FTD/ALS poly(GA) RAN-translated proteins cause neurotoxicity by inducing ER stress. Acta Neuropathol. 2014;128:505-24 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200
Peng Z, Zhang N, Chandra D, Homanics G, Olsen R, Houser C. Altered localization of the ? subunit of the GABAA receptor in the thalamus of ?4 subunit knockout mice. Neurochem Res. 2014;39:1104-17 pubmed publisher
  • immunocytochemistry; mouse
Wang H, Yu X, Guo C, Zuo D, Fisher P, Subjeck J, et al. Enhanced endoplasmic reticulum entry of tumor antigen is crucial for cross-presentation induced by dendritic cell-targeted vaccination. J Immunol. 2013;191:6010-21 pubmed publisher
  • immunohistochemistry; rat; 1:250
Cox D, Racca C. Differential dendritic targeting of AMPA receptor subunit mRNAs in adult rat hippocampal principal neurons and interneurons. J Comp Neurol. 2013;521:1954-2007 pubmed publisher
  • western blot; mouse
Favero C, Henshaw R, Grimsley Myers C, Shrestha A, Beier D, Dwyer N. Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain. J Comp Neurol. 2013;521:677-96 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:400
Sakakibara S, Nakadate K, Tanaka Nakadate S, Yoshida K, Nogami S, Shirataki H, et al. Developmental and spatial expression pattern of alpha-taxilin in the rat central nervous system. J Comp Neurol. 2008;511:65-80 pubmed publisher
Taneike M, Nishida K, Omiya S, Zarrinpashneh E, Misaka T, Kitazume Taneike R, et al. mTOR Hyperactivation by Ablation of Tuberous Sclerosis Complex 2 in the Mouse Heart Induces Cardiac Dysfunction with the Increased Number of Small Mitochondria Mediated through the Down-Regulation of Autophagy. PLoS ONE. 2016;11:e0152628 pubmed publisher
Laura R, Dong D, Reynolds W, Maki R. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation. PLoS ONE. 2016;11:e0149391 pubmed publisher
Niwa Y, Suzuki T, Dohmae N, Simizu S. Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells. Mol Biol Cell. 2016;27:744-56 pubmed publisher
Brunati M, Perucca S, Han L, Cattaneo A, Consolato F, Andolfo A, et al. The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin. elife. 2015;4:e08887 pubmed publisher
Ulbrich L, Favaloro F, Trobiani L, Marchetti V, Patel V, Pascucci T, et al. Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system. Biochem J. 2016;473:423-34 pubmed publisher
Koga T, Suico M, Shimasaki S, Watanabe E, Kai Y, Koyama K, et al. Endoplasmic Reticulum (ER) Stress Induces Sirtuin 1 (SIRT1) Expression via the PI3K-Akt-GSK3β Signaling Pathway and Promotes Hepatocellular Injury. J Biol Chem. 2015;290:30366-74 pubmed publisher
Koyama S, Omura T, Yonezawa A, Imai S, Nakagawa S, Nakagawa T, et al. Gefitinib and Erlotinib Lead to Phosphorylation of Eukaryotic Initiation Factor 2 Alpha Independent of Epidermal Growth Factor Receptor in A549 Cells. PLoS ONE. 2015;10:e0136176 pubmed publisher
Teng Y, Rezvani K, De Biasi M. UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP. Biochem Pharmacol. 2015;97:518-530 pubmed publisher
Pinho A, Bensellam M, Wauters E, Rees M, Giry Laterriere M, Mawson A, et al. Pancreas-Specific Sirt1-Deficiency in Mice Compromises Beta-Cell Function without Development of Hyperglycemia. PLoS ONE. 2015;10:e0128012 pubmed publisher
Nam D, Han J, Lee T, Shishido T, Lim J, Kim G, et al. CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction. J Mol Cell Cardiol. 2015;85:168-77 pubmed publisher
Dunst S, Kazimiers T, von Zadow F, Jambor H, Sagner A, Brankatschk B, et al. Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila. Dev Cell. 2015;33:351-65 pubmed publisher
Hotokezaka Y, Katayama I, van Leyen K, Nakamura T. GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses. Cell Death Dis. 2015;6:e1719 pubmed publisher
Nanao T, Koike M, Yamaguchi J, Sasaki M, Uchiyama Y. Cellular localization and tissue distribution of endogenous DFCP1 protein. Biomed Res. 2015;36:121-33 pubmed publisher
Ferron M, LaCombe J, Germain A, Oury F, Karsenty G. GGCX and VKORC1 inhibit osteocalcin endocrine functions. J Cell Biol. 2015;208:761-76 pubmed publisher
Merulla J, Soldà T, Molinari M. A novel UGGT1 and p97-dependent checkpoint for native ectodomains with ionizable intramembrane residue. Mol Biol Cell. 2015;26:1532-42 pubmed publisher
Chen L, Xie Z, Turkson S, Zhuang X. A53T human α-synuclein overexpression in transgenic mice induces pervasive mitochondria macroautophagy defects preceding dopamine neuron degeneration. J Neurosci. 2015;35:890-905 pubmed publisher
Gehrke S, Wu Z, Klinkenberg M, Sun Y, Auburger G, Guo S, et al. PINK1 and Parkin control localized translation of respiratory chain component mRNAs on mitochondria outer membrane. Cell Metab. 2015;21:95-108 pubmed publisher
Dorweiler B, Grechowa I, Wallrath A, Vahl C, Horke S. Activation of the proapoptotic unfolded protein response in plaques of the human carotid artery. Eur J Vasc Endovasc Surg. 2014;48:248-57 pubmed publisher
Takayanagi T, Crawford K, Kobayashi T, Obama T, Tsuji T, Elliott K, et al. Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase. Clin Sci (Lond). 2014;126:785-94 pubmed publisher
Boslem E, Weir J, Macintosh G, Sue N, Cantley J, Meikle P, et al. Alteration of endoplasmic reticulum lipid rafts contributes to lipotoxicity in pancreatic ?-cells. J Biol Chem. 2013;288:26569-82 pubmed publisher
Park E, Kim G, Yun S, Lim H, Hong Y, Kwon S, et al. Analysis of the endoplasmic reticulum subproteome in the livers of type 2 diabetic mice. Int J Mol Sci. 2012;13:17230-43 pubmed publisher
Putz U, Howitt J, Lackovic J, Foot N, Kumar S, Silke J, et al. Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins. J Biol Chem. 2008;283:32621-7 pubmed publisher
Duriez M, Rossignol J, Sitterlin D. The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated degradation pathway. J Biol Chem. 2008;283:32352-60 pubmed publisher
Hosoi T, Sasaki M, Miyahara T, Hashimoto C, Matsuo S, Yoshii M, et al. Endoplasmic reticulum stress induces leptin resistance. Mol Pharmacol. 2008;74:1610-9 pubmed publisher
Okamura Y, Saga Y. Pofut1 is required for the proper localization of the Notch receptor during mouse development. Mech Dev. 2008;125:663-73 pubmed publisher
Ito D, Suzuki N. Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases. Ann Neurol. 2007;61:237-50 pubmed
product information
Product ID :
ADI-SPA-827
Catalog Number :
ADI-SPA-827-J
Product Name :
KDEL monoclonal antibody (10C3)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
1 mg
list of Pubmed id :
26499802, 26265139, 26046931, 26027784, 25942626, 25880086, 25876663, 25694454, 25609609, 25565208
Clone :
10C3
Isotype :
IgG2a
SPECIES REACTIVITY :
Yeast, Plant, Mammalian, Insect, Avian
Background :
The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.
ALTnames :
Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine
Immunogen :
Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
Specificity :
Recognizes proteins containing the KDEL sequence.
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.
Application Summary :
ELISA, FC, ICC, IF, IHC (PS), IP, WB, Electron microscopy
Storage :
-20°C
more info or order :
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.