KDEL monoclonal antibody (10C3) (DyLight™ 488 conjugate) | Enzo Life Sciences ADI-SPA-827-488-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

KDEL monoclonal antibody (10C3) (DyLight™ 488 conjugate)

catalog :

ADI-SPA-827-488-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

DyLight488

clone name :

10C3

The same clone is also sold as:

Abcam: ab115638

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 2	Reisen F, Sauty De Chalon A, Pfeifer M, Zhang X, Gabriel D, Selzer P. Linking phenotypes and modes of action through high-content screen fingerprints. Assay Drug Dev Technol. 2015;13:415-27 pubmed publisher
	Taneike M, Nishida K, Omiya S, Zarrinpashneh E, Misaka T, Kitazume Taneike R, et al. mTOR Hyperactivation by Ablation of Tuberous Sclerosis Complex 2 in the Mouse Heart Induces Cardiac Dysfunction with the Increased Number of Small Mitochondria Mediated through the Down-Regulation of Autophagy. PLoS ONE. 2016;11:e0152628 pubmed publisher
	Laura R, Dong D, Reynolds W, Maki R. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation. PLoS ONE. 2016;11:e0149391 pubmed publisher
	Niwa Y, Suzuki T, Dohmae N, Simizu S. Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells. Mol Biol Cell. 2016;27:744-56 pubmed publisher
	Brunati M, Perucca S, Han L, Cattaneo A, Consolato F, Andolfo A, et al. The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin. elife. 2015;4:e08887 pubmed publisher
	Ulbrich L, Favaloro F, Trobiani L, Marchetti V, Patel V, Pascucci T, et al. Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system. Biochem J. 2016;473:423-34 pubmed publisher
	Koyama S, Omura T, Yonezawa A, Imai S, Nakagawa S, Nakagawa T, et al. Gefitinib and Erlotinib Lead to Phosphorylation of Eukaryotic Initiation Factor 2 Alpha Independent of Epidermal Growth Factor Receptor in A549 Cells. PLoS ONE. 2015;10:e0136176 pubmed publisher
	Ferron M, LaCombe J, Germain A, Oury F, Karsenty G. GGCX and VKORC1 inhibit osteocalcin endocrine functions. J Cell Biol. 2015;208:761-76 pubmed publisher
	Boslem E, Weir J, Macintosh G, Sue N, Cantley J, Meikle P, et al. Alteration of endoplasmic reticulum lipid rafts contributes to lipotoxicity in pancreatic ?-cells. J Biol Chem. 2013;288:26569-82 pubmed publisher
	Park E, Kim G, Yun S, Lim H, Hong Y, Kwon S, et al. Analysis of the endoplasmic reticulum subproteome in the livers of type 2 diabetic mice. Int J Mol Sci. 2012;13:17230-43 pubmed publisher

product information

Product ID :

ADI-SPA-827-488

Catalog Number :

ADI-SPA-827-488-D

Product Name :

KDEL monoclonal antibody (10C3) (DyLight™ 488 conjugate)

Host species :

Mouse

Clonality :

Monoclonal

Conjugation :

DyLight™ 488

Product Type :

Antibody

Size :

50 µg

Clone :

10C3

Isotype :

IgG2a

SPECIES REACTIVITY :

Yeast, Plant, Mammalian, Insect, Avian

Background :

The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.

ALTnames :

Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine

Immunogen :

Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.

Specificity :

Recognizes proteins containing the KDEL sequence.

Buffer :

Liquid. In PBS containing 0.09% sodium azide.

Uses :

Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.

Application Summary :

Storage :

+4°C

more info or order :

Enzo Life Sciences product webpage