HSC70/HSP70 monoclonal antibody (BB70) | Enzo Life Sciences ADI-SPA-822-F product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSC70/HSP70 monoclonal antibody (BB70)

catalog :

ADI-SPA-822-F

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BB70

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation, other

more info or order :

Enzo Life Sciences product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1a	Ortega F, Roefs M, De Miguel Pérez D, Kooijmans S, de Jong O, Sluijter J, et al. Interfering with endolysosomal trafficking enhances release of bioactive exosomes. Nanomedicine. 2019;:102014 pubmed publisher
immunoprecipitation; human; fig 3	Huang K, Chen Z, Jiang Y, Akare S, Kolber Simonds D, Condon K, et al. Apratoxin A Shows Novel Pancreas-Targeting Activity through the Binding of Sec 61. Mol Cancer Ther. 2016;15:1208-16 pubmed publisher
other; human; loading ...; fig st1	Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
	Narayan M, Seeley K, Jinwal U. Identification and quantitative analysis of cellular proteins affected by treatment with withaferin a using a SILAC-based proteomics approach. J Ethnopharmacol. 2015;175:86-92 pubmed publisher
	Proia D, Smith D, Zhang J, Jimenez J, Sang J, Ogawa L, et al. HSP90 Inhibitor-SN-38 Conjugate Strategy for Targeted Delivery of Topoisomerase I Inhibitor to Tumors. Mol Cancer Ther. 2015;14:2422-32 pubmed publisher
	DÃaz F, Orobio R, Chavarriaga P, Toro Perea N. Differential expression patterns among heat-shock protein genes and thermal responses in the whitefly Bemisia tabaci (MEAM 1). J Therm Biol. 2015;52:199-207 pubmed publisher
	Pearce M, Spartz E, Hong W, Luo L, Kopito R. Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain. Nat Commun. 2015;6:6768 pubmed publisher
	Sun J, Li W, Jang W, Nayyar N, Sutton M, Edgerton M. Uptake of the antifungal cationic peptide Histatin 5 by Candida albicans Ssa2p requires binding to non-conventional sites within the ATPase domain. Mol Microbiol. 2008;70:1246-60 pubmed
	Goldbaum O, Jensen P, Richter Landsberg C. The expression of tubulin polymerization promoting protein TPPP/p25alpha is developmentally regulated in cultured rat brain oligodendrocytes and affected by proteolytic stress. Glia. 2008;56:1736-46 pubmed publisher
	Rank N, Bruce D, McMillan D, Barclay C, Dahlhoff E. Phosphoglucose isomerase genotype affects running speed and heat shock protein expression after exposure to extreme temperatures in a montane willow beetle. J Exp Biol. 2007;210:750-64 pubmed
	Miki K, Eddy E. Tumor necrosis factor receptor 1 is an ATPase regulated by silencer of death domain. Mol Cell Biol. 2002;22:2536-43 pubmed
	Sakahira H, Nagata S. Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase. J Biol Chem. 2002;277:3364-70 pubmed
	Walters T, Ryan K, Mason P. Regional distribution of Hsp70 in the CNS of young and old food-restricted rats following hyperthermia. Brain Res Bull. 2001;55:367-74 pubmed

product information

Product ID :

ADI-SPA-822

Catalog Number :

ADI-SPA-822-F

Product Name :

HSC70/HSP70 monoclonal antibody (BB70)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

200 µg

list of Pubmed id :

26392330, 26271675, 26267515, 25866135, 19006817, 18563798, 17297136, 11909948, 11724800, 11489344

Clone :

BB70

Isotype :

IgG2a

SPECIES REACTIVITY :

Human, Mouse, Rat, Yeast, Sponge, Rabbit, Housefly, Drosophila

Background :

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

ALTnames :

Heat shock protein 70, Hsc70, Hsp73

Immunogen :

Chicken Hsp70/Hsp90 complex.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~72kDa by Western blot.

Application Summary :

IHC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage