product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSC70/HSP70 monoclonal antibody (BB70)
catalog :
ADI-SPA-822-D
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BB70

The same clone is also sold as:
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunoprecipitation, other
more info or order :
Enzo Life Sciences product webpage
citations: 14
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 1a
Ortega F, Roefs M, De Miguel Pérez D, Kooijmans S, de Jong O, Sluijter J, et al. Interfering with endolysosomal trafficking enhances release of bioactive exosomes. Nanomedicine. 2019;:102014 pubmed publisher
  • immunoprecipitation; human; fig 3
Huang K, Chen Z, Jiang Y, Akare S, Kolber Simonds D, Condon K, et al. Apratoxin A Shows Novel Pancreas-Targeting Activity through the Binding of Sec 61. Mol Cancer Ther. 2016;15:1208-16 pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
Narayan M, Seeley K, Jinwal U. Identification and quantitative analysis of cellular proteins affected by treatment with withaferin a using a SILAC-based proteomics approach. J Ethnopharmacol. 2015;175:86-92 pubmed publisher
Proia D, Smith D, Zhang J, Jimenez J, Sang J, Ogawa L, et al. HSP90 Inhibitor-SN-38 Conjugate Strategy for Targeted Delivery of Topoisomerase I Inhibitor to Tumors. Mol Cancer Ther. 2015;14:2422-32 pubmed publisher
Díaz F, Orobio R, Chavarriaga P, Toro Perea N. Differential expression patterns among heat-shock protein genes and thermal responses in the whitefly Bemisia tabaci (MEAM 1). J Therm Biol. 2015;52:199-207 pubmed publisher
Pearce M, Spartz E, Hong W, Luo L, Kopito R. Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain. Nat Commun. 2015;6:6768 pubmed publisher
Sun J, Li W, Jang W, Nayyar N, Sutton M, Edgerton M. Uptake of the antifungal cationic peptide Histatin 5 by Candida albicans Ssa2p requires binding to non-conventional sites within the ATPase domain. Mol Microbiol. 2008;70:1246-60 pubmed
Goldbaum O, Jensen P, Richter Landsberg C. The expression of tubulin polymerization promoting protein TPPP/p25alpha is developmentally regulated in cultured rat brain oligodendrocytes and affected by proteolytic stress. Glia. 2008;56:1736-46 pubmed publisher
Rank N, Bruce D, McMillan D, Barclay C, Dahlhoff E. Phosphoglucose isomerase genotype affects running speed and heat shock protein expression after exposure to extreme temperatures in a montane willow beetle. J Exp Biol. 2007;210:750-64 pubmed
Miki K, Eddy E. Tumor necrosis factor receptor 1 is an ATPase regulated by silencer of death domain. Mol Cell Biol. 2002;22:2536-43 pubmed
Sakahira H, Nagata S. Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase. J Biol Chem. 2002;277:3364-70 pubmed
Walters T, Ryan K, Mason P. Regional distribution of Hsp70 in the CNS of young and old food-restricted rats following hyperthermia. Brain Res Bull. 2001;55:367-74 pubmed
Croute F, Beau B, Arrabit C, Gaubin Y, Delmas F, Murat J, et al. Pattern of stress protein expression in human lung cell-line A549 after short- or long-term exposure to cadmium. Environ Health Perspect. 2000;108:55-60 pubmed
product information
Product ID :
ADI-SPA-822
Catalog Number :
ADI-SPA-822-D
Product Name :
HSC70/HSP70 monoclonal antibody (BB70)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
50 µg
list of Pubmed id :
26392330, 26271675, 26267515, 25866135, 19006817, 18563798, 17297136, 11909948, 11724800, 11489344
Clone :
BB70
Isotype :
IgG2a
SPECIES REACTIVITY :
Human, Mouse, Rat, Yeast, Sponge, Rabbit, Housefly, Drosophila
Background :
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
ALTnames :
Heat shock protein 70, Hsc70, Hsp73
Immunogen :
Chicken Hsp70/Hsp90 complex.
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~72kDa by Western blot.
Application Summary :
IHC, IP, WB
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.