product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSC70/HSP70 monoclonal antibody (N27F3-4) (PE conjugate)
catalog :
ADI-SPA-820PE-F
quantity :
200 µg
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
N27F3-4

The same clone is also sold as:
reactivity :
mouse
application :
western blot, flow cytometry
more info or order :
Enzo Life Sciences product webpage
citations: 14
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; fig 4b
Görtz D, Braun G, Maruta Y, Djudjaj S, van Roeyen C, Martin I, et al. Anti-interleukin-6 therapy through application of a monogenic protein inhibitor via gene delivery. Sci Rep. 2015;5:14685 pubmed publisher
Liu S, Hossinger A, Hofmann J, Denner P, Vorberg I. Horizontal Transmission of Cytosolic Sup35 Prions by Extracellular Vesicles. MBio. 2016;7: pubmed publisher
Gaponova A, Nikonova A, Deneka A, Kopp M, Kudinov A, Skobeleva N, et al. A Novel HSP90 Inhibitor-Drug Conjugate to SN38 Is Highly Effective in Small Cell Lung Cancer. Clin Cancer Res. 2016;22:5120-5129 pubmed publisher
Matamala N, Vargas M, González Cámpora R, Arias J, Menéndez P, Andrés León E, et al. MicroRNA deregulation in triple negative breast cancer reveals a role of miR-498 in regulating BRCA1 expression. Oncotarget. 2016;7:20068-79 pubmed publisher
Alam S, Rochon D. Cucumber Necrosis Virus Recruits Cellular Heat Shock Protein 70 Homologs at Several Stages of Infection. J Virol. 2015;90:3302-17 pubmed publisher
Franco L, Terrinca J, Rodríguez A, Espino J, Pariente J. Extracellular heat shock proteins protect U937 cells from H2O2-induced apoptotic cell death. Mol Cell Biochem. 2016;412:19-26 pubmed publisher
Jiang Q, Zhang Z, Li S, Wang Z, Ma Y, Hu Y. Defective heat shock factor 1 inhibits the growth of fibrosarcoma derived from simian virus 40/T antigen‑transformed MEF cells. Mol Med Rep. 2015;12:6517-26 pubmed publisher
Di Giancamillo A, Rossi R, Pastorelli G, Deponti D, Carollo V, Casamassima D, et al. The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets. J Anim Sci. 2015;93:2849-59 pubmed publisher
Chen H, Zhang X, Cheng Y, Abdelnasir A, Tang S, Kemper N, et al. Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo. Genet Mol Res. 2015;14:1994-2005 pubmed publisher
Zhou Z, Doggett T, Sene A, Apte R, Ferguson T. Autophagy supports survival and phototransduction protein levels in rod photoreceptors. Cell Death Differ. 2015;22:488-98 pubmed publisher
Chen H, Adam A, Cheng Y, Tang S, Hartung J, Bao E. Localization and expression of heat shock protein 70 with rat myocardial cell damage induced by heat stress in vitro and in vivo. Mol Med Rep. 2015;11:2276-84 pubmed publisher
Cao F, Xu L, Zhang X, Wang Y, Li M, Uzan G, et al. IP-FCM platform detects the existence and regulator-caused dissociation of components in naturally assembled HSP90 complex. Cytometry A. 2014;85:359-67 pubmed publisher
Chatain N, Ziegler P, Fahrenkamp D, Jost E, Moriggl R, Schmitz Van de Leur H, et al. Src family kinases mediate cytoplasmic retention of activated STAT5 in BCR-ABL-positive cells. Oncogene. 2013;32:3587-97 pubmed publisher
Peng H, Morishima Y, Pratt W, Osawa Y. Modulation of heme/substrate binding cleft of neuronal nitric-oxide synthase (nNOS) regulates binding of Hsp90 and Hsp70 proteins and nNOS ubiquitination. J Biol Chem. 2012;287:1556-65 pubmed publisher
product information
Product ID :
ADI-SPA-820PE
Catalog Number :
ADI-SPA-820PE-F
Product Name :
HSC70/HSP70 monoclonal antibody (N27F3-4) (PE conjugate)
Host species :
Mouse
Clonality :
Monoclonal
Conjugation :
PE
Product Type :
Antibody
Size :
200 µg
Clone :
N27F3-4
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat, Xenopus, Sheep, Rabbit, Plant, Porcine, Monkey, Hamster, Guinea pig, Fish, Dog, Chicken, Bovine, Beluga
Background :
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
ALTnames :
Heat shock protein 70, Hsc70, Hsp73
Immunogen :
Purified HSC70 and HSP70 isolated from human HeLa cells.
Buffer :
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Application Summary :
FC
Storage :
+4°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.