HSC70/HSP70 monoclonal antibody (N27F3-4) | Enzo Life Sciences ADI-SPA-820-F product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSC70/HSP70 monoclonal antibody (N27F3-4)

catalog :

ADI-SPA-820-F

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N27F3-4

reactivity :

Nicotiana benthamiana, human, mouse, rat, dogs, chicken, bovine

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 6a	Tamaki Y, Shodai A, Morimura T, Hikiami R, Minamiyama S, Ayaki T, et al. Elimination of TDP-43 inclusions linked to amyotrophic lateral sclerosis by a misfolding-specific intrabody with dual proteolytic signals. Sci Rep. 2018;8:6030 pubmed publisher
western blot; Nicotiana benthamiana; fig 1b	Alam S, Rochon D. Evidence that Hsc70 Is Associated with Cucumber Necrosis Virus Particles and Plays a Role in Particle Disassembly. J Virol. 2017;91: pubmed publisher
western blot; mouse; 1:1000; fig 4	Liu S, Hossinger A, Hofmann J, Denner P, Vorberg I. Horizontal Transmission of Cytosolic Sup35 Prions by Extracellular Vesicles. MBio. 2016;7: pubmed publisher
western blot; mouse; fig 4b	GÃ¶rtz D, Braun G, Maruta Y, Djudjaj S, van Roeyen C, Martin I, et al. Anti-interleukin-6 therapy through application of a monogenic protein inhibitor via gene delivery. Sci Rep. 2015;5:14685 pubmed publisher
western blot; mouse; 1:2000	Zhou Z, Doggett T, Sene A, Apte R, Ferguson T. Autophagy supports survival and phototransduction protein levels in rod photoreceptors. Cell Death Differ. 2015;22:488-98 pubmed publisher
western blot; mouse; 1:1000	Smithline Z, Nikonova A, Hensley H, Cai K, Egleston B, Proia D, et al. Inhibiting heat shock protein 90 (HSP90) limits the formation of liver cysts induced by conditional deletion of Pkd1 in mice. PLoS ONE. 2014;9:e114403 pubmed publisher
western blot; rat	Peng H, Morishima Y, Pratt W, Osawa Y. Modulation of heme/substrate binding cleft of neuronal nitric-oxide synthase (nNOS) regulates binding of Hsp90 and Hsp70 proteins and nNOS ubiquitination. J Biol Chem. 2012;287:1556-65 pubmed publisher
	Gaponova A, Nikonova A, Deneka A, Kopp M, Kudinov A, Skobeleva N, et al. A Novel HSP90 Inhibitor-Drug Conjugate to SN38 Is Highly Effective in Small Cell Lung Cancer. Clin Cancer Res. 2016;22:5120-5129 pubmed publisher
	Matamala N, Vargas M, GonzÃ¡lez CÃ¡mpora R, Arias J, MenÃ©ndez P, AndrÃ©s LeÃ³n E, et al. MicroRNA deregulation in triple negative breast cancer reveals a role of miR-498 in regulating BRCA1 expression. Oncotarget. 2016;7:20068-79 pubmed publisher
	Alam S, Rochon D. Cucumber Necrosis Virus Recruits Cellular Heat Shock Protein 70 Homologs at Several Stages of Infection. J Virol. 2015;90:3302-17 pubmed publisher
	Franco L, Terrinca J, RodrÃguez A, Espino J, Pariente J. Extracellular heat shock proteins protect U937 cells from H2O2-induced apoptotic cell death. Mol Cell Biochem. 2016;412:19-26 pubmed publisher
	Jiang Q, Zhang Z, Li S, Wang Z, Ma Y, Hu Y. Defective heat shock factor 1 inhibits the growth of fibrosarcoma derived from simian virus 40/T antigenâ€‘transformed MEF cells. Mol Med Rep. 2015;12:6517-26 pubmed publisher
	Di Giancamillo A, Rossi R, Pastorelli G, Deponti D, Carollo V, Casamassima D, et al. The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets. J Anim Sci. 2015;93:2849-59 pubmed publisher
	Mulaudzi Masuku T, Mutepe R, Mukhoro O, Faro A, Ndimba B. Identification and characterization of a heat-inducible Hsp70 gene from Sorghum bicolor which confers tolerance to thermal stress. Cell Stress Chaperones. 2015;20:793-804 pubmed publisher
	Chen H, Zhang X, Cheng Y, Abdelnasir A, Tang S, Kemper N, et al. Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo. Genet Mol Res. 2015;14:1994-2005 pubmed publisher
	Chen H, Adam A, Cheng Y, Tang S, Hartung J, Bao E. Localization and expression of heat shock protein 70 with rat myocardial cell damage induced by heat stress in vitro and in vivo. Mol Med Rep. 2015;11:2276-84 pubmed publisher
	Cao F, Xu L, Zhang X, Wang Y, Li M, Uzan G, et al. IP-FCM platform detects the existence and regulator-caused dissociation of components in naturally assembled HSP90 complex. Cytometry A. 2014;85:359-67 pubmed publisher
	Chatain N, Ziegler P, Fahrenkamp D, Jost E, Moriggl R, Schmitz Van de Leur H, et al. Src family kinases mediate cytoplasmic retention of activated STAT5 in BCR-ABL-positive cells. Oncogene. 2013;32:3587-97 pubmed publisher
	Yaglom J, Gabai V, Sherman M. High levels of heat shock protein Hsp72 in cancer cells suppress default senescence pathways. Cancer Res. 2007;67:2373-81 pubmed
	Camarda G, Siepi F, Pajalunga D, Bernardini C, Rossi R, Montecucco A, et al. A pRb-independent mechanism preserves the postmitotic state in terminally differentiated skeletal muscle cells. J Cell Biol. 2004;167:417-23 pubmed
	Sakahira H, Nagata S. Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase. J Biol Chem. 2002;277:3364-70 pubmed
	Chen D, Androlewicz M. Heat shock protein 70 moderately enhances peptide binding and transport by the transporter associated with antigen processing. Immunol Lett. 2001;75:143-8 pubmed
	Porrello A, Cerone M, Coen S, Gurtner A, Fontemaggi G, Cimino L, et al. p53 regulates myogenesis by triggering the differentiation activity of pRb. J Cell Biol. 2000;151:1295-304 pubmed
	Dressel R, Elsner L, Quentin T, Walter L, Gunther E. Heat shock protein 70 is able to prevent heat shock-induced resistance of target cells to CTL. J Immunol. 2000;164:2362-71 pubmed
	Minota S, Cameron B, Welch W, Winfield J. Autoantibodies to the constitutive 73-kD member of the hsp70 family of heat shock proteins in systemic lupus erythematosus. J Exp Med. 1988;168:1475-80 pubmed
	Kost S, Smith D, Sullivan W, Welch W, Toft D. Binding of heat shock proteins to the avian progesterone receptor. Mol Cell Biol. 1989;9:3829-38 pubmed
	Allan G, Leng X, Tsai S, Weigel N, Edwards D, Tsai M, et al. Hormone and antihormone induce distinct conformational changes which are central to steroid receptor activation. J Biol Chem. 1992;267:19513-20 pubmed

product information

Product ID :

ADI-SPA-820

Catalog Number :

ADI-SPA-820-F

Product Name :

HSC70/HSP70 monoclonal antibody (N27F3-4)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

200 µg

list of Pubmed id :

26072391, 25571975, 17332370, 11724800, 11137139, 10679071, 3171482, 2779568, 1326555

Clone :

N27F3-4

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Xenopus, Sheep, Rabbit, Plant, Porcine, Monkey, Hamster, Guinea pig, Fish, Dog, Chicken, Bovine, Beluga

Background :

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

ALTnames :

Heat shock protein 70, Hsc70, Hsp73

Immunogen :

Purified HSC70 and HSP70 isolated from human HeLa cells.

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~72-73kDa by Western blot.

Application Summary :

FC, IHC (PS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage