product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSC70/HSP73 monoclonal antibody (1B5) (biotin conjugate)
catalog :
ADI-SPA-815B-D
quantity :
50 µg
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
1B5

The same clone is also sold as:
reactivity :
human, mouse, rat, dogs, chicken, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Enzo Life Sciences product webpage
citations: 7
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 1
Dhamad A, Zhou Z, Zhou J, Du Y. Systematic Proteomic Identification of the Heat Shock Proteins (Hsp) that Interact with Estrogen Receptor Alpha (ER?) and Biochemical Characterization of the ER?-Hsp70 Interaction. PLoS ONE. 2016;11:e0160312 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:300; fig 2
Trieb K, Sulzbacher I, Kubista B. Recurrence rate and progression of chondrosarcoma is correlated with heat shock protein expression. Oncol Lett. 2016;11:521-524 pubmed
  • western blot; mouse; 1:2000; fig 4
Ferreira J, Soares A, Ramalho J, Pereira P, Girao H. K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy. Sci Rep. 2015;5:10210 pubmed publisher
Veit G, Oliver K, Apaja P, Perdomo D, Bidaud Meynard A, Lin S, et al. Ribosomal Stalk Protein Silencing Partially Corrects the ΔF508-CFTR Functional Expression Defect. PLoS Biol. 2016;14:e1002462 pubmed publisher
Andreeva N, Zatsepina O, Garbuz D, Evgen ev M, Belyavsky A. Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells. Cell Stress Chaperones. 2016;21:727-33 pubmed publisher
Gleixner A, Posimo J, Pant D, Henderson M, Leak R. Astrocytes Surviving Severe Stress Can Still Protect Neighboring Neurons from Proteotoxic Injury. Mol Neurobiol. 2016;53:4939-60 pubmed publisher
Honegger A, Schilling D, Bastian S, Sponagel J, Kuryshev V, Sultmann H, et al. Dependence of intracellular and exosomal microRNAs on viral E6/E7 oncogene expression in HPV-positive tumor cells. PLoS Pathog. 2015;11:e1004712 pubmed publisher
product information
Product ID :
ADI-SPA-815B
Catalog Number :
ADI-SPA-815B-D
Product Name :
HSC70/HSP73 monoclonal antibody (1B5) (biotin conjugate)
Host species :
Rat
Clonality :
Monoclonal
Conjugation :
Biotin
Product Type :
Antibody
Size :
50 µg
Clone :
1B5
Isotype :
IgG2b
SPECIES REACTIVITY :
Human, Mouse, Rat, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Dog, Chicken, Bovine
Background :
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
ALTnames :
Heat shock protein 70, Hsc70, Hsp73
Immunogen :
Native hamster Hsc70 (Hsp73).
Buffer :
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Uses :
Detects a band of ~73kDa by Western blot.
Application Summary :
ELISA, FC, ICC, IHC, IP, WB, Electron microscopy
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.