product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSC70/HSP73 monoclonal antibody (1B5)
catalog :
ADI-SPA-815-D
quantity :
50 µg
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
1B5

The same clone is also sold as:
reactivity :
Garra rufa, human, mouse, rat, dogs, chicken, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Enzo Life Sciences product webpage
citations: 32
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 1d
Mailer R, Gisterå A, Polyzos K, Ketelhuth D, Hansson G. Hypercholesterolemia Enhances T Cell Receptor Signaling and Increases the Regulatory T Cell Population. Sci Rep. 2017;7:15655 pubmed publisher
  • western blot; human; fig 1
Dhamad A, Zhou Z, Zhou J, Du Y. Systematic Proteomic Identification of the Heat Shock Proteins (Hsp) that Interact with Estrogen Receptor Alpha (ER?) and Biochemical Characterization of the ER?-Hsp70 Interaction. PLoS ONE. 2016;11:e0160312 pubmed publisher
  • western blot; mouse; 1:5000; fig 1
Stewart M, Plante I, Penuela S, Laird D. Loss of Panx1 Impairs Mammary Gland Development at Lactation: Implications for Breast Tumorigenesis. PLoS ONE. 2016;11:e0154162 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:300; fig 2
Trieb K, Sulzbacher I, Kubista B. Recurrence rate and progression of chondrosarcoma is correlated with heat shock protein expression. Oncol Lett. 2016;11:521-524 pubmed
  • western blot; human; fig s3
Kowal J, Arras G, Colombo M, Jouve M, Morath J, Primdal Bengtson B, et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016;113:E968-77 pubmed publisher
  • western blot; mouse; fig 3
Hyenne V, Apaydin A, Rodriguez D, Spiegelhalter C, Hoff Yoessle S, Diem M, et al. RAL-1 controls multivesicular body biogenesis and exosome secretion. J Cell Biol. 2015;211:27-37 pubmed publisher
  • western blot; mouse; 1:2000; fig 4
Ferreira J, Soares A, Ramalho J, Pereira P, Girao H. K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy. Sci Rep. 2015;5:10210 pubmed publisher
  • western blot; human; 1:1000; fig 7
Honegger A, Schilling D, Bastian S, Sponagel J, Kuryshev V, Sultmann H, et al. Dependence of intracellular and exosomal microRNAs on viral E6/E7 oncogene expression in HPV-positive tumor cells. PLoS Pathog. 2015;11:e1004712 pubmed publisher
  • western blot; Garra rufa
Oksala N, Ekmekçi F, Ozsoy E, Kirankaya S, Kokkola T, Emecen G, et al. Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress. Redox Biol. 2014;3:25-8 pubmed publisher
  • western blot; human
Suico M, Fukuda R, Miyakita R, Koyama K, Taura M, Shuto T, et al. The transcription factor MEF/Elf4 is dually modulated by p53-MDM2 axis and MEF-MDM2 autoregulatory mechanism. J Biol Chem. 2014;289:26143-54 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
  • immunocytochemistry; human
Kitamura A, Inada N, Kubota H, Matsumoto G, Kinjo M, Morimoto R, et al. Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1. Genes Cells. 2014;19:209-24 pubmed publisher
  • western blot; mouse; 1:2500; fig 4
Reina C, Nabet B, Young P, Pittman R. Basal and stress-induced Hsp70 are modulated by ataxin-3. Cell Stress Chaperones. 2012;17:729-42 pubmed publisher
Kopp Y, Lang W, Schuster T, Martínez Limón A, Hofbauer H, Ernst R, et al. CHIP as a membrane-shuttling proteostasis sensor. elife. 2017;6: pubmed publisher
Seo J, Park J, Lee E, Vo T, Choi H, Kim J, et al. ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation. Nat Commun. 2016;7:12882 pubmed publisher
Santio N, Landor S, Vahtera L, Ylä Pelto J, Paloniemi E, Imanishi S, et al. Phosphorylation of Notch1 by Pim kinases promotes oncogenic signaling in breast and prostate cancer cells. Oncotarget. 2016;7:43220-43238 pubmed publisher
Veit G, Oliver K, Apaja P, Perdomo D, Bidaud Meynard A, Lin S, et al. Ribosomal Stalk Protein Silencing Partially Corrects the ΔF508-CFTR Functional Expression Defect. PLoS Biol. 2016;14:e1002462 pubmed publisher
Andreeva N, Zatsepina O, Garbuz D, Evgen ev M, Belyavsky A. Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells. Cell Stress Chaperones. 2016;21:727-33 pubmed publisher
Gleixner A, Posimo J, Pant D, Henderson M, Leak R. Astrocytes Surviving Severe Stress Can Still Protect Neighboring Neurons from Proteotoxic Injury. Mol Neurobiol. 2016;53:4939-60 pubmed publisher
Schmitz Rohmer D, Probst S, Yang Z, Laurent F, Stadler M, Zuniga A, et al. NDR Kinases Are Essential for Somitogenesis and Cardiac Looping during Mouse Embryonic Development. PLoS ONE. 2015;10:e0136566 pubmed publisher
Helenius T, Misiorek J, Nyström J, Fortelius L, Habtezion A, Liao J, et al. Keratin 8 absence down-regulates colonocyte HMGCS2 and modulates colonic ketogenesis and energy metabolism. Mol Biol Cell. 2015;26:2298-310 pubmed publisher
Heimdal K, Sanchez Guixé M, Aukrust I, Bollerslev J, Bruland O, Jablonski G, et al. STUB1 mutations in autosomal recessive ataxias - evidence for mutation-specific clinical heterogeneity. Orphanet J Rare Dis. 2014;9:146 pubmed publisher
Gleixner A, Pulugulla S, Pant D, Posimo J, Crum T, Leak R. Impact of aging on heat shock protein expression in the substantia nigra and striatum of the female rat. Cell Tissue Res. 2014;357:43-54 pubmed publisher
Suzuki S, Shuto T, Sato T, Kaneko M, Takada T, Suico M, et al. Inhibition of post-translational N-glycosylation by HRD1 that controls the fate of ABCG5/8 transporter. Sci Rep. 2014;4:4258 pubmed publisher
Honegger A, Leitz J, Bulkescher J, Hoppe Seyler K, Hoppe Seyler F. Silencing of human papillomavirus (HPV) E6/E7 oncogene expression affects both the contents and the amounts of extracellular microvesicles released from HPV-positive cancer cells. Int J Cancer. 2013;133:1631-42 pubmed publisher
Bento C, Marques F, Fernandes R, Pereira P. Methylglyoxal alters the function and stability of critical components of the protein quality control. PLoS ONE. 2010;5:e13007 pubmed publisher
Moerdyk Schauwecker M, Hwang S, Grdzelishvili V. Analysis of virion associated host proteins in vesicular stomatitis virus using a proteomics approach. Virol J. 2009;6:166 pubmed publisher
Li P, Itoh N, Watanabe M, Shi Y, Liu P, Yang H, et al. Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens. J Virol. 2009;83:37-46 pubmed publisher
Lu H, Sun T, Matsuzaki T, Yi X, Eswara J, Bouley R, et al. Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking. J Biol Chem. 2007;282:28721-32 pubmed
Tetzlaff J, Tanzer L, Jones K. Exogenous androgen treatment delays the stress response following hamster facial nerve injury. J Neuroendocrinol. 2007;19:383-9 pubmed
Meinander A, Söderström T, Kaunisto A, Poukkula M, Sistonen L, Eriksson J. Fever-like hyperthermia controls T Lymphocyte persistence by inducing degradation of cellular FLIPshort. J Immunol. 2007;178:3944-53 pubmed
Yamano T, Murata S, Shimbara N, Tanaka N, Chiba T, Tanaka K, et al. Two distinct pathways mediated by PA28 and hsp90 in major histocompatibility complex class I antigen processing. J Exp Med. 2002;196:185-96 pubmed
product information
Product ID :
ADI-SPA-815
Catalog Number :
ADI-SPA-815-D
Product Name :
HSC70/HSP73 monoclonal antibody (1B5)
Host species :
Rat
Clonality :
Monoclonal
Product Type :
Antibody
Size :
50 µg
list of Pubmed id :
26305214, 25958982, 25904331, 25760330, 25258038, 24723229, 23526637, 22777893, 20885985, 18945763
Clone :
1B5
Isotype :
IgG2a
SPECIES REACTIVITY :
Human, Mouse, Rat, Turtle, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Dog, Chicken, Bovine
Background :
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
ALTnames :
Heat shock protein 70, Hsc70, Hsp73
Immunogen :
Native hamster Hsc70 (Hsp73).
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~73kDa by Western blot.
Application Summary :
ELISA, FC, ICC, IF, IHC (PS), IP, WB, Electron microscopy
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.