HSC70/HSP73 monoclonal antibody (1B5) (DyLight™ 488 conjugate) | Enzo Life Sciences ADI-SPA-815-488-E product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSC70/HSP73 monoclonal antibody (1B5) (DyLight™ 488 conjugate)

catalog :

ADI-SPA-815-488-E

quantity :

100 µg

clonality :

monoclonal

host :

rat

conjugate :

DyLight488

clone name :

1B5

reactivity :

human, mouse, rat, dogs, chicken, bovine

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Enzo Life Sciences product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1	Dhamad A, Zhou Z, Zhou J, Du Y. Systematic Proteomic Identification of the Heat Shock Proteins (Hsp) that Interact with Estrogen Receptor Alpha (ER?) and Biochemical Characterization of the ER?-Hsp70 Interaction. PLoS ONE. 2016;11:e0160312 pubmed publisher
immunohistochemistry - paraffin section; human; 1:300; fig 2	Trieb K, Sulzbacher I, Kubista B. Recurrence rate and progression of chondrosarcoma is correlated with heat shock protein expression. Oncol Lett. 2016;11:521-524 pubmed
western blot; mouse; 1:2000; fig 4	Ferreira J, Soares A, Ramalho J, Pereira P, Girao H. K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy. Sci Rep. 2015;5:10210 pubmed publisher
	Veit G, Oliver K, Apaja P, Perdomo D, Bidaud Meynard A, Lin S, et al. Ribosomal Stalk Protein Silencing Partially Corrects the ΔF508-CFTR Functional Expression Defect. PLoS Biol. 2016;14:e1002462 pubmed publisher
	Andreeva N, Zatsepina O, Garbuz D, Evgen ev M, Belyavsky A. Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells. Cell Stress Chaperones. 2016;21:727-33 pubmed publisher
	Gleixner A, Posimo J, Pant D, Henderson M, Leak R. Astrocytes Surviving Severe Stress Can Still Protect Neighboring Neurons from Proteotoxic Injury. Mol Neurobiol. 2016;53:4939-60 pubmed publisher
	Honegger A, Schilling D, Bastian S, Sponagel J, Kuryshev V, Sultmann H, et al. Dependence of intracellular and exosomal microRNAs on viral E6/E7 oncogene expression in HPV-positive tumor cells. PLoS Pathog. 2015;11:e1004712 pubmed publisher

product information

Product ID :

ADI-SPA-815-488

Catalog Number :

ADI-SPA-815-488-E

Product Name :

HSC70/HSP73 monoclonal antibody (1B5) (DyLight™ 488 conjugate)

Host species :

Rat

Clonality :

Monoclonal

Conjugation :

DyLight™ 488

Product Type :

Antibody

Size :

100 µg

Clone :

1B5

Isotype :

IgG2a

SPECIES REACTIVITY :

Human, Mouse, Rat, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Dog, Chicken, Bovine

Background :

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

ALTnames :

Heat shock protein 70, Hsc70, Hsp73

Immunogen :

Native hamster Hsc70 (Hsp73).

Buffer :

Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.

Uses :

Detects a band of ~73kDa by Western blot.

Application Summary :

Storage :

+4°C

more info or order :

Enzo Life Sciences product webpage