HSP70/HSP72 monoclonal antibody (N15F2-5) | Enzo Life Sciences ADI-SPA-8133-D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSP70/HSP72 monoclonal antibody (N15F2-5)

catalog :

ADI-SPA-8133-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N15F2-5

reactivity :

human

application :

western blot

citations: 1

Reference
Kishor A, White E, Matsangos A, Yan Z, Tandukar B, Wilson G. Hsp70's RNA-binding and mRNA-stabilizing activities are independent of its protein chaperone functions. J Biol Chem. 2017;292:14122-14133 pubmed publisher

product information

Product ID :

ADI-SPA-8133

Catalog Number :

ADI-SPA-8133-D

Product Name :

HSP70/HSP72 monoclonal antibody (N15F2-5)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

Clone :

N15F2-5

Isotype :

IgG1

SPECIES REACTIVITY :

Human, E. coli

Background :

The 70 kDa heat shock protein Hsp70 belongs to the Hsp70 family of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. Hsc70 shares close biochemical and biological ties to Hsp70, and also belongs to the Hsp70 family. These proteins include cognate members found within major intracellular compartments and highly inducible isoforms predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family function as molecular chaperones involved in such cellular functions as protein folding, transport, maturation and degradation, operating in an ATP-dependent manner. The molecular chaperones of the Hsp70 family recognize and bind to nascent polypeptide chains or partially folded intermediates of proteins, preventing their aggregation and misfolding, and the binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. Data demonstrates that with a ubiquitin-like domain at its amino terminus and its association with the 26S proteosome in HeLa cells, Bag-1 modulates the chaperone activity of Hsc70 and Hsp70. These findings reveal Bag-1's role as a physical link between the Hsc70/Hsp70 chaperone system and the proteasome. Experimental data also shows that the ATPase domain and the substrate-binding domain of Hsp70 (or Hsc70) cooperate to form a co-chaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp), essential for normal neurotransmitter release.

ALTnames :

Hsp70, HspA1A, Heat shock protein 70, HspA1B, Hsp72

Immunogen :

Native human Hsp70.

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~70kDa by Western blot.

Application Summary :

Storage :

-20°C