product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSP70/HSP72 monoclonal antibody (C92F3A-5) (PE conjugate)
catalog :
ADI-SPA-810PE-D
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
C92F3A-5
reactivity :
human, mouse
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 49
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:400; loading ...; fig 3d
Wang T, Wu C, Ouzounov D, Gu W, Xia F, Kim M, et al. Quantitative analysis of 1300-nm three-photon calcium imaging in the mouse brain. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; human; 1:50; fig 7
  • western blot; human; 1:10,000; fig 3
  • western blot; mouse; 1:10,000; fig 1
Lin P, Folorunso O, Taglialatela G, Pierce A. Overexpression of heat shock factor 1 maintains TAR DNA binding protein 43 solubility via induction of inducible heat shock protein 70 in cultured cells. J Neurosci Res. 2016;94:671-82 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Larabee C, Georgescu C, Wren J, Plafker S. Expression profiling of the ubiquitin conjugating enzyme UbcM2 in murine brain reveals modest age-dependent decreases in specific neurons. BMC Neurosci. 2015;16:76 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Zhou J, Wan J, Gao X, Zhang X, Jaffrey S, Qian S. Dynamic m(6)A mRNA methylation directs translational control of heat shock response. Nature. 2015;526:591-4 pubmed publisher
Takagi R, Ogasawara R, Tsutaki A, Nakazato K, Ishii N. Regional adaptation of collagen in skeletal muscle to repeated bouts of strenuous eccentric exercise. Pflugers Arch. 2016;468:1565-72 pubmed publisher
Xie F, Zhan R, Yan L, Gong J, Zhao Y, Ma J, et al. Diet-induced elevation of circulating HSP70 may trigger cell adhesion and promote the development of atherosclerosis in rats. Cell Stress Chaperones. 2016;21:907-14 pubmed publisher
Sclip A, Bacaj T, Giam L, Sudhof T. Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction. PLoS ONE. 2016;11:e0158295 pubmed publisher
Podgorski K, Ranganathan G. Brain heating induced by near-infrared lasers during multiphoton microscopy. J Neurophysiol. 2016;116:1012-23 pubmed publisher
Chooi W, Chan B. Compression loading-induced stress responses in intervertebral disc cells encapsulated in 3D collagen constructs. Sci Rep. 2016;6:26449 pubmed publisher
Veit G, Oliver K, Apaja P, Perdomo D, Bidaud Meynard A, Lin S, et al. Ribosomal Stalk Protein Silencing Partially Corrects the ΔF508-CFTR Functional Expression Defect. PLoS Biol. 2016;14:e1002462 pubmed publisher
Kasza A, Hunya A, Frank Z, Fulop F, Torok Z, Balogh G, et al. Dihydropyridine Derivatives Modulate Heat Shock Responses and have a Neuroprotective Effect in a Transgenic Mouse Model of Alzheimer's Disease. J Alzheimers Dis. 2016;53:557-71 pubmed publisher
Lavinsky D, Wang J, Huie P, Dalal R, Lee S, Lee D, et al. Nondamaging Retinal Laser Therapy: Rationale and Applications to the Macula. Invest Ophthalmol Vis Sci. 2016;57:2488-500 pubmed publisher
Gutierrez E, Seebacher N, Arzuman L, Kovacevic Z, Lane D, Richardson V, et al. Lysosomal membrane stability plays a major role in the cytotoxic activity of the anti-proliferative agent, di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT). Biochim Biophys Acta. 2016;1863:1665-81 pubmed publisher
Andreeva N, Zatsepina O, Garbuz D, Evgen ev M, Belyavsky A. Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells. Cell Stress Chaperones. 2016;21:727-33 pubmed publisher
Kim J, Son Y, Jung M, Jeong Y, Kim S, Lee S, et al. Geranylgeranylacetone alleviates radiation-induced lung injury by inhibiting epithelial-to-mesenchymal transition signaling. Mol Med Rep. 2016;13:4666-70 pubmed publisher
Tamura Y, Matsunaga Y, Kitaoka Y, Hatta H. Effects of Heat Stress Treatment on Age-dependent Unfolded Protein Response in Different Types of Skeletal Muscle. J Gerontol A Biol Sci Med Sci. 2017;72:299-308 pubmed publisher
Peppler W, Anderson Z, Sutton C, Rector R, Wright D. Voluntary wheel running attenuates lipopolysaccharide-induced liver inflammation in mice. Am J Physiol Regul Integr Comp Physiol. 2016;310:R934-42 pubmed publisher
Saito Y, Nakagawa T, Kakihana A, Nakamura Y, Nabika T, Kasai M, et al. Yeast Two-Hybrid and One-Hybrid Screenings Identify Regulators of hsp70 Gene Expression. J Cell Biochem. 2016;117:2109-17 pubmed publisher
Frara N, Abdelmagid S, Tytell M, Amin M, Popoff S, Safadi F, et al. Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping. BMC Musculoskelet Disord. 2016;17:34 pubmed publisher
Asano Y, Kawase T, Okabe A, Tsutsumi S, Ichikawa H, Tatebe S, et al. IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis. Sci Rep. 2016;6:19174 pubmed publisher
Shi L, Gehin T, Chevolot Y, Souteyrand E, Mangé A, Solassol J, et al. Anti-heat shock protein autoantibody profiling in breast cancer using customized protein microarray. Anal Bioanal Chem. 2016;408:1497-506 pubmed publisher
Negishi T, Matsumoto M, Kojima M, Asai R, Kanehira T, Sakaguchi F, et al. Diphenylarsinic Acid Induced Activation of Cultured Rat Cerebellar Astrocytes: Phosphorylation of Mitogen-Activated Protein Kinases, Upregulation of Transcription Factors, and Release of Brain-Active Cytokines. Toxicol Sci. 2016;150:74-83 pubmed publisher
Sarkar S, Leo B, Carranza C, Chen S, Rivas Santiago C, Porter A, et al. Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification. PLoS ONE. 2015;10:e0143077 pubmed publisher
Clemente Vicario F, Alvarez C, ROWELL J, Roy S, London C, Kisseberth W, et al. Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines. PLoS ONE. 2015;10:e0142007 pubmed publisher
Briassouli E, Tzanoudaki M, Goukos D, Routsi C, Nanas S, Vardas K, et al. Glutamine may repress the weak LPS and enhance the strong heat shock induction of monocyte and lymphocyte HSP72 proteins but may not modulate the HSP72 mRNA in patients with sepsis or trauma. Biomed Res Int. 2015;2015:806042 pubmed publisher
Kern P, Cramp R, Seebacher F, Ghanizadeh Kazerouni E, Franklin C. Plasticity of protective mechanisms only partially explains interactive effects of temperature and UVR on upper thermal limits. Comp Biochem Physiol A Mol Integr Physiol. 2015;190:75-82 pubmed publisher
Xiong R, Zhou W, Siegel D, Kitson R, Freed C, Moody C, et al. A Novel Hsp90 Inhibitor Activates Compensatory Heat Shock Protein Responses and Autophagy and Alleviates Mutant A53T α-Synuclein Toxicity. Mol Pharmacol. 2015;88:1045-54 pubmed publisher
Jiang Q, Zhang Z, Li S, Wang Z, Ma Y, Hu Y. Defective heat shock factor 1 inhibits the growth of fibrosarcoma derived from simian virus 40/T antigen‑transformed MEF cells. Mol Med Rep. 2015;12:6517-26 pubmed publisher
Smith B, Simpson D, Miller R, Erdman J, O Brien W. Contrast Ultrasound Imaging Does Not Affect Heat Shock Protein 70 Expression in Cholesterol-Fed Rabbit Aorta. J Ultrasound Med. 2015;34:1209-16 pubmed publisher
Jeong Y, Jung M, Son Y, Jang J, Lee Y, Kim S, et al. Coniferyl aldehyde attenuates radiation enteropathy by inhibiting cell death and promoting endothelial cell function. PLoS ONE. 2015;10:e0128552 pubmed publisher
Alexandrova E, Yallowitz A, Li D, Xu S, Schulz R, Proia D, et al. Improving survival by exploiting tumour dependence on stabilized mutant p53 for treatment. Nature. 2015;523:352-6 pubmed publisher
Kim J, Lee Y, Choi D, Yi H. Expression of Heat Shock Proteins (HSPs) in Aged Skeletal Muscles Depends on the Frequency and Duration of Exercise Training. J Sports Sci Med. 2015;14:347-53 pubmed
Tamura Y, Kitaoka Y, Matsunaga Y, Hoshino D, Hatta H. Daily heat stress treatment rescues denervation-activated mitochondrial clearance and atrophy in skeletal muscle. J Physiol. 2015;593:2707-20 pubmed publisher
Saito K, Kukita K, Kutomi G, Okuya K, Asanuma H, Tabeya T, et al. Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE. Eur J Immunol. 2015;45:2028-41 pubmed publisher
Schilling D, Kühnel A, Tetzlaff F, Konrad S, Multhoff G. NZ28-induced inhibition of HSF1, SP1 and NF-κB triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells. Cancer Immunol Immunother. 2015;64:599-608 pubmed publisher
Schilling D, Kühnel A, Konrad S, Tetzlaff F, Bayer C, Yaglom J, et al. Sensitizing tumor cells to radiation by targeting the heat shock response. Cancer Lett. 2015;360:294-301 pubmed publisher
Morlé A, Garrido C, Micheau O. Hyperthermia restores apoptosis induced by death receptors through aggregation-induced c-FLIP cytosolic depletion. Cell Death Dis. 2015;6:e1633 pubmed publisher
Liu Y, Yu Y, Chu H, Bing D, Wang S, Zhou L, et al. 17-DMAG induces Hsp70 and protects the auditory hair cells from kanamycin ototoxicity in vitro. Neurosci Lett. 2015;588:72-7 pubmed publisher
Kabbaj F, Lu S, Faouzi M, Meddah B, Proksch P, Cherrah Y, et al. Bioactive metabolites from Chaetomium aureum: structure elucidation and inhibition of the Hsp90 machine chaperoning activity. Bioorg Med Chem. 2015;23:126-31 pubmed publisher
Davis A, Qiao S, Lesson J, Rojo de la Vega M, Park S, Seanez C, et al. The quinone methide aurin is a heat shock response inducer that causes proteotoxic stress and Noxa-dependent apoptosis in malignant melanoma cells. J Biol Chem. 2015;290:1623-38 pubmed publisher
Ramirez V, Krueger W, Aneskievich B. TNIP1 reduction of HSPA6 gene expression occurs in promoter regions lacking binding sites for known TNIP1-repressed transcription factors. Gene. 2015;555:430-7 pubmed publisher
Leyk J, Goldbaum O, Noack M, Richter Landsberg C. Inhibition of HDAC6 modifies tau inclusion body formation and impairs autophagic clearance. J Mol Neurosci. 2015;55:1031-46 pubmed publisher
Roufayel R, Johnston D, Mosser D. The elimination of miR-23a in heat-stressed cells promotes NOXA-induced cell death and is prevented by HSP70. Cell Death Dis. 2014;5:e1546 pubmed publisher
Dang V, Tanabe K, Tanaka Y, Tokumoto N, Misumi T, Saeki Y, et al. Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation. PLoS ONE. 2014;9:e110748 pubmed publisher
Ramirez V, Stamatis M, Shmukler A, Aneskievich B. Basal and stress-inducible expression of HSPA6 in human keratinocytes is regulated by negative and positive promoter regions. Cell Stress Chaperones. 2015;20:95-107 pubmed publisher
Agulla J, Brea D, Campos F, Sobrino T, Argibay B, Al Soufi W, et al. In vivo theranostics at the peri-infarct region in cerebral ischemia. Theranostics. 2013;4:90-105 pubmed publisher
Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
Mitra S, Giesselman B, De Jesús Andino F, Foster T. Tumor response to mTHPC-mediated photodynamic therapy exhibits strong correlation with extracellular release of HSP70. Lasers Surg Med. 2011;43:632-43 pubmed publisher
Needham P, Mikoluk K, Dhakarwal P, Khadem S, Snyder A, Subramanya A, et al. The thiazide-sensitive NaCl cotransporter is targeted for chaperone-dependent endoplasmic reticulum-associated degradation. J Biol Chem. 2011;286:43611-21 pubmed publisher
product information
Product ID :
ADI-SPA-810PE
Catalog Number :
ADI-SPA-810PE-D
Product Name :
HSP70/HSP72 monoclonal antibody (C92F3A-5) (PE conjugate)
Host species :
Mouse
Clonality :
Monoclonal
Conjugation :
PE
Product Type :
Antibody
Size :
50 µg
Clone :
C92F3A-5
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Fish, Drosophila, Dog, Chicken, Bovine, Beluga
Background :
The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
ALTnames :
Hsp70, HspA1A, Heat shock protein 70, HspA1B, Hsp72
Immunogen :
Native human Hsp70.
Buffer :
Liquid. In PBS containing 0.09% sodium azide.
Uses :
Detects a band of ~70kDa by Western blot.
Application Summary :
FC
Storage :
+4°C
more info or order :
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.