HSP60 monoclonal antibody (LK-2) | Enzo Life Sciences ADI-SPA-807-E product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSP60 monoclonal antibody (LK-2)

catalog :

ADI-SPA-807-E

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LK-2

The same clone is also sold as:

QED Bioscience: 11101
Abnova: MAB6622
Rockland Immunochemicals: 200-301-F61
Abcam: ab59458
OriGene: TA326374
Novus Biologicals: NBP2-34671-0.1mg, NBP2-32867-0.1mg
StressMarq Biosciences: SMC-111

reactivity :

, African green monkey, human, mouse, rat, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Enzo Life Sciences product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; African green monkey; 1:5000; loading ...; fig 1d immunocytochemistry; human; 1:5000; loading ...; fig 1b immunocytochemistry; mouse; 1:5000; loading ...; fig 2b	Newman L, Schiavon C, Zhou C, Kahn R. The abundance of the ARL2 GTPase and its GAP, ELMOD2, at mitochondria are modulated by the fusogenic activity of mitofusins and stressors. PLoS ONE. 2017;12:e0175164 pubmed publisher
immunocytochemistry; human; 1:5000; loading ...; fig 2	Newman L, Schiavon C, Kahn R. Plasmids for variable expression of proteins targeted to the mitochondrial matrix or intermembrane space. Cell Logist. 2016;6:e1247939 pubmed publisher
western blot; human; 1:4000; loading ...; fig 1	Nilsen T, Thorsen L, Kirkegaard C, Ugelstad I, Fossa S, Raastad T. The effect of strength training on muscle cellular stress in prostate cancer patients on ADT. Endocr Connect. 2016;5:74-82 pubmed publisher
blocking or activating experiments; mouse; fig 3	Heiserman J, Chen L, Kim B, Kim S, Tran A, Siebenborn N, et al. TLR4 mutation and HSP60-induced cell death in adult mouse cardiac myocytes. Cell Stress Chaperones. 2015;20:527-35 pubmed publisher
	Fischer F, Filippis C, Osiewacz H. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci Rep. 2015;5:12697 pubmed publisher
	Ortega A, Sala S, Espinosa E, Gonzalez Baron M, Cuezva J. HuR and the bioenergetic signature of breast cancer: a low tumor expression of the RNA-binding protein predicts a higher risk of disease recurrence. Carcinogenesis. 2008;29:2053-61 pubmed publisher
	Alam T, Kanki T, Muta T, Ukaji K, Abe Y, Nakayama H, et al. Human mitochondrial DNA is packaged with TFAM. Nucleic Acids Res. 2003;31:1640-5 pubmed
	Wang X, Wang D, Zhu W, Delrahim K, Dolnak D, Rapaport M. Studies characterizing 60 kda autoantibodies in subjects with schizophrenia. Biol Psychiatry. 2003;53:361-75 pubmed
	Satoh M, Hamamoto T, Seo N, Kagawa Y, Endo H. Differential sublocalization of the dynamin-related protein OPA1 isoforms in mitochondria. Biochem Biophys Res Commun. 2003;300:482-93 pubmed
	Tabeta K, Yamazaki K, Hotokezaka H, Yoshie H, Hara K. Elevated humoral immune response to heat shock protein 60 (hsp60) family in periodontitis patients. Clin Exp Immunol. 2000;120:285-93 pubmed
	Belles C, Kuhl A, Nosheny R, Carding S. Plasma membrane expression of heat shock protein 60 in vivo in response to infection. Infect Immun. 1999;67:4191-200 pubmed
	Giraldo P, Neuer A, Korneeva I, Ribeiro Filho A, Simoes J, Witkin S. Vaginal heat shock protein expression in symptom-free women with a history of recurrent vulvovaginitis. Am J Obstet Gynecol. 1999;180:524-9 pubmed
	Zhang Y, Ohashi N, Lee E, Tamura A, Rikihisa Y. Ehrlichia sennetsu groE operon and antigenic properties of the GroEL homolog. FEMS Immunol Med Microbiol. 1997;18:39-46 pubmed
	Munoz M, Jeremias J, Witkin S. The 60 kDa heat shock protein in human semen: relationship with antibodies to spermatozoa and Chlamydia trachomatis. Hum Reprod. 1996;11:2600-3 pubmed
	Boog C, de Graeff Meeder E, Lucassen M, van der Zee R, Voorhorst Ogink M, van Kooten P, et al. Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis. J Exp Med. 1992;175:1805-10 pubmed

product information

Product ID :

ADI-SPA-807

Catalog Number :

ADI-SPA-807-E

Product Name :

HSP60 monoclonal antibody (LK-2)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

18687667, 12626705, 12614989, 12504110, 10792378, 10417191, 10076122, 9215585, 9021358, 1316935

Clone :

LK-2

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Ehrlichia sennetsu, Rabbit, Plant, Porcine, Monkey, Hamster, Guinea pig, Dog, Chicken, C. elegans, Bovine

Background :

Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.

ALTnames :

Chaperonin 60, CPN60, HspD1, heat shock protein 60

Immunogen :

Recombinant human HSP60.

Buffer :

Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol.

Uses :

Detects a band of ~60kDa by Western blot.

Application Summary :

ELISA, FC, IHC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage