product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSP60 monoclonal antibody (LK-1)
catalog :
ADI-SPA-806-J
quantity :
1 mg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
LK-1

The same clone is also sold as:
reactivity :
Garra rufa, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
Enzo Life Sciences product webpage
citations: 14
Published Application/Species/Sample/DilutionReference
  • western blot; rat; 1:1200
Van Laar V, Roy N, Liu A, Rajprohat S, Arnold B, Dukes A, et al. Glutamate excitotoxicity in neurons triggers mitochondrial and endoplasmic reticulum accumulation of Parkin, and, in the presence of N-acetyl cysteine, mitophagy. Neurobiol Dis. 2015;74:180-93 pubmed publisher
  • western blot; Garra rufa
Oksala N, Ekmekçi F, Ozsoy E, Kirankaya S, Kokkola T, Emecen G, et al. Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress. Redox Biol. 2014;3:25-8 pubmed publisher
  • western blot; rat; 1:2000
Moura C, Lollo P, Morato P, Nisishima L, Carneiro E, Amaya Farfan J. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS ONE. 2014;9:e83437 pubmed publisher
Pharaoh G, Pulliam D, Hill S, Sataranatarajan K, Van Remmen H. Ablation of the mitochondrial complex IV assembly protein Surf1 leads to increased expression of the UPR(MT) and increased resistance to oxidative stress in primary cultures of fibroblasts. Redox Biol. 2016;8:430-8 pubmed publisher
Kasza A, Hunya A, Frank Z, Fulop F, Torok Z, Balogh G, et al. Dihydropyridine Derivatives Modulate Heat Shock Responses and have a Neuroprotective Effect in a Transgenic Mouse Model of Alzheimer's Disease. J Alzheimers Dis. 2016;53:557-71 pubmed publisher
Tamura Y, Matsunaga Y, Kitaoka Y, Hatta H. Effects of Heat Stress Treatment on Age-dependent Unfolded Protein Response in Different Types of Skeletal Muscle. J Gerontol A Biol Sci Med Sci. 2017;72:299-308 pubmed publisher
Wichmann H, Vocke F, Brinkhoff T, Simon M, Richter Landsberg C. Cytotoxic Effects of Tropodithietic Acid on Mammalian Clonal Cell Lines of Neuronal and Glial Origin. Mar Drugs. 2015;13:7113-23 pubmed publisher
Kratochwill K, Bender T, Lichtenauer A, Herzog R, Tarantino S, Bialas K, et al. Cross-omics comparison of stress responses in mesothelial cells exposed to heat- versus filter-sterilized peritoneal dialysis fluids. Biomed Res Int. 2015;2015:628158 pubmed publisher
Kumar Singh M, Janardhan Reddy P, Sreedhar A, Tiwari P. Molecular characterization and expression analysis of hsp60 gene homologue of sheep blowfly, Lucilia cuprina. J Therm Biol. 2015;52:24-37 pubmed publisher
Tamura Y, Kitaoka Y, Matsunaga Y, Hoshino D, Hatta H. Daily heat stress treatment rescues denervation-activated mitochondrial clearance and atrophy in skeletal muscle. J Physiol. 2015;593:2707-20 pubmed publisher
Tamura Y, Matsunaga Y, Masuda H, Takahashi Y, Takahashi Y, Terada S, et al. Postexercise whole body heat stress additively enhances endurance training-induced mitochondrial adaptations in mouse skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2014;307:R931-43 pubmed publisher
Zhang X, He M, Cheng L, Chen Y, Zhou L, Zeng H, et al. Elevated heat shock protein 60 levels are associated with higher risk of coronary heart disease in Chinese. Circulation. 2008;118:2687-93 pubmed publisher
Horiguchi Y, Araki M, Motojima K. Identification and characterization of the ER/lipid droplet-targeting sequence in 17beta-hydroxysteroid dehydrogenase type 11. Arch Biochem Biophys. 2008;479:121-30 pubmed publisher
Ren J, Agata N, Chen D, Li Y, Yu W, Huang L, et al. Human MUC1 carcinoma-associated protein confers resistance to genotoxic anticancer agents. Cancer Cell. 2004;5:163-75 pubmed
product information
Product ID :
ADI-SPA-806
Catalog Number :
ADI-SPA-806-J
Product Name :
HSP60 monoclonal antibody (LK-1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
1 mg
list of Pubmed id :
26633426, 26495307, 26267495, 25080501, 19106391, 18804447, 14998492
Clone :
LK-1
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat, Xenopus, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Drosophila, Dog, Chicken, Bovine
Background :
Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.
ALTnames :
Chaperonin 60, CPN60, HspD1, heat shock protein 60
Immunogen :
Recombinant human Hsp60.
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~60kDa by Western blot.
Application Summary :
ELISA, FC, IF, IHC (PS), IP, WB
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.