product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSP27 monoclonal antibody (G3.1)
catalog :
ADI-SPA-800-D
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
G3.1

The same clone is also sold as:
reactivity :
human, mouse, rat, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
Enzo Life Sciences product webpage
citations: 22
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 2
Zeng X, Han I, Abd El Barr M, Aljuboori Z, Anderson J, Chi J, et al. The Effects of Thermal Preconditioning on Oncogenic and Intraspinal Cord Growth Features of Human Glioma Cells. Cell Transplant. 2016;25:2099-2109 pubmed publisher
  • western blot; human; fig s3
Morlé A, Garrido C, Micheau O. Hyperthermia restores apoptosis induced by death receptors through aggregation-induced c-FLIP cytosolic depletion. Cell Death Dis. 2015;6:e1633 pubmed publisher
  • western blot; mouse
Dang V, Tanabe K, Tanaka Y, Tokumoto N, Misumi T, Saeki Y, et al. Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation. PLoS ONE. 2014;9:e110748 pubmed publisher
  • immunohistochemistry; rat; 1:1000
Moloney T, Hyland R, O Toole D, Paucard A, Kirik D, O Doherty A, et al. Heat shock protein 70 reduces ?-synuclein-induced predegenerative neuronal dystrophy in the ?-synuclein viral gene transfer rat model of Parkinson's disease. CNS Neurosci Ther. 2014;20:50-8 pubmed publisher
  • western blot; human
Mao R, Rubio V, Chen H, Bai L, Mansour O, Shi Z. OLA1 protects cells in heat shock by stabilizing HSP70. Cell Death Dis. 2013;4:e491 pubmed publisher
  • western blot; human
Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
Sevin M, Kubovcakova L, Pernet N, Causse S, Vitte F, Villeval J, et al. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. Nat Commun. 2018;9:1431 pubmed publisher
Yang X, Hu Z, Fan S, Zhang Q, Zhong Y, Guo D, et al. Picornavirus 2A protease regulates stress granule formation to facilitate viral translation. PLoS Pathog. 2018;14:e1006901 pubmed publisher
Homa J, Ortmann W, Kolaczkowska E. Conservative Mechanisms of Extracellular Trap Formation by Annelida Eisenia andrei: Serine Protease Activity Requirement. PLoS ONE. 2016;11:e0159031 pubmed publisher
Qvit N, Joshi A, Cunningham A, Ferreira J, Mochly Rosen D. Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death. J Biol Chem. 2016;291:13608-21 pubmed publisher
Asano Y, Kawase T, Okabe A, Tsutsumi S, Ichikawa H, Tatebe S, et al. IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis. Sci Rep. 2016;6:19174 pubmed publisher
Shi L, Gehin T, Chevolot Y, Souteyrand E, Mangé A, Solassol J, et al. Anti-heat shock protein autoantibody profiling in breast cancer using customized protein microarray. Anal Bioanal Chem. 2016;408:1497-506 pubmed publisher
Xiong R, Zhou W, Siegel D, Kitson R, Freed C, Moody C, et al. A Novel Hsp90 Inhibitor Activates Compensatory Heat Shock Protein Responses and Autophagy and Alleviates Mutant A53T α-Synuclein Toxicity. Mol Pharmacol. 2015;88:1045-54 pubmed publisher
Lash L, Putt D, Jankovich A. Glutathione Levels and Susceptibility to Chemically Induced Injury in Two Human Prostate Cancer Cell Lines. Molecules. 2015;20:10399-414 pubmed publisher
Mu T, Ong D, Wang Y, Balch W, Yates J, Segatori L, et al. Chemical and biological approaches synergize to ameliorate protein-folding diseases. Cell. 2008;134:769-81 pubmed publisher
Othmen Z, Golli E, Abid Essefi S, Bacha H. Cytotoxicity effects induced by Zearalenone metabolites, alpha Zearalenol and beta Zearalenol, on cultured Vero cells. Toxicology. 2008;252:72-7 pubmed publisher
Vidyasagar A, Reese S, Acun Z, Hullett D, Djamali A. HSP27 is involved in the pathogenesis of kidney tubulointerstitial fibrosis. Am J Physiol Renal Physiol. 2008;295:F707-16 pubmed publisher
Paulsen G, Vissing K, Kalhovde J, Ugelstad I, Bayer M, Kadi F, et al. Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans. Am J Physiol Regul Integr Comp Physiol. 2007;293:R844-53 pubmed
Sakamoto H, Mashima T, Yamamoto K, Tsuruo T. Modulation of heat-shock protein 27 (Hsp27) anti-apoptotic activity by methylglyoxal modification. J Biol Chem. 2002;277:45770-5 pubmed
Radice S, Ferraris M, Marabini L, Chiesara E. Estrogenic activity of procymidone in primary cultured rainbow trout hepatocytes (Oncorhynchus mykiss). Toxicol In Vitro. 2002;16:475-80 pubmed
Jonak C, Klosner G, Kokesch C, FOdinger D, HOnigsmann H, Trautinger F. Subcorneal colocalization of the small heat shock protein, hsp27, with keratins and proteins of the cornified cell envelope. Br J Dermatol. 2002;147:13-9 pubmed
Garcia J, Wang P, Schaphorst K, Becker P, Borbiev T, Liu F, et al. Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability. FASEB J. 2002;16:1064-76 pubmed
product information
Product ID :
ADI-SPA-800
Catalog Number :
ADI-SPA-800-D
Product Name :
HSP27 monoclonal antibody (G3.1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
50 µg
list of Pubmed id :
26056813, 18775310, 18755238, 18596079, 17522120, 12226095, 12110288, 12100179, 12100179, 12087068
Clone :
G3.1
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat, Monkey, Fish, Bovine
Background :
Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.
ALTnames :
HspB1, Heat shock protein 27
Immunogen :
Native human Hsp27.
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~27kDa by Western blot.
Application Summary :
ELISA, ICC, IHC (PS), IP, WB, in vitro Assay, Electron microscopy
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.