Calreticulin monoclonal antibody (FMC 75) (PE conjugate) | Enzo Life Sciences ADI-SPA-601PE-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Calreticulin monoclonal antibody (FMC 75) (PE conjugate)

catalog :

ADI-SPA-601PE-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

FMC 75

The same clone is also sold as:

Novus Biologicals: NBP1-97502-0.05mg
Abcam: ab22683
Invitrogen: MA1-91034

reactivity :

human

application :

flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	Martínez Torres A, Quiney C, Attout T, Boullet H, Herbi L, Vela L, et al. CD47 agonist peptides induce programmed cell death in refractory chronic lymphocytic leukemia B cells via PLCÎ³1 activation: evidence from mice and humans. PLoS Med. 2015;12:e1001796 pubmed publisher
	MikyÅ¡kovÃ¡ R, Å tÄ›pÃ¡nek I, IndrovÃ¡ M, BieblovÃ¡ J, Å ÃmovÃ¡ J, TruxovÃ¡ I, et al. Dendritic cells pulsed with tumor cells killed by high hydrostatic pressure induce strong immune responses and display therapeutic effects both in murine TC-1 and TRAMP-C2 tumors when combined with docetaxel chemotherapy. Int J Oncol. 2016;48:953-64 pubmed publisher
	Kuriyama T, Takenaka K, Kohno K, Yamauchi T, Daitoku S, Yoshimoto G, et al. Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis. Blood. 2012;120:4058-67 pubmed publisher
	Suzuki Y, Mimura K, Yoshimoto Y, Watanabe M, Ohkubo Y, Izawa S, et al. Immunogenic tumor cell death induced by chemoradiotherapy in patients with esophageal squamous cell carcinoma. Cancer Res. 2012;72:3967-76 pubmed publisher
	Chao M, Jaiswal S, Weissman Tsukamoto R, Alizadeh A, Gentles A, Volkmer J, et al. Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med. 2010;2:63ra94 pubmed publisher

product information

Product ID :

ADI-SPA-601PE

Catalog Number :

ADI-SPA-601PE-D

Product Name :

Calreticulin monoclonal antibody (FMC 75) (PE conjugate)

Host species :

Mouse

Clonality :

Monoclonal

Conjugation :

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

25734483, 22990013, 22700877, 21178137

Clone :

FMC 75

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Monkey

Background :

The multifunctional, multi -compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins, as well as a Ca2+-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein), Crt contains the ER-retrieval sequence, KDEL, and is the solub le paralog of the ER membrane protein Calnexin (Cnx). Crt's three domains include a 180 residue N-terminal domain, a proline-rich Pdomainresidues 189 -288) that binds Ca2+ with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P -domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N -glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression.

ALTnames :

CALR, CRT

Immunogen :

Recombinant calreticulin.

Buffer :

Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.

Uses :

Predicted MW of ~63kDa.

Application Summary :

Storage :

+4°C

more info or order :

Enzo Life Sciences product webpage