Calreticulin monoclonal antibody (FMC 75) | Enzo Life Sciences ADI-SPA-601-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Calreticulin monoclonal antibody (FMC 75)

catalog :

ADI-SPA-601-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

FMC 75

The same clone is also sold as:

Novus Biologicals: NBP1-97502-0.05mg
Abcam: ab22683
Invitrogen: MA1-91034

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, other

more info or order :

Enzo Life Sciences product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
other; human; loading ...; fig st1	Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
immunocytochemistry; mouse; fig 1	MikyÅ¡kovÃ¡ R, Å tÄ›pÃ¡nek I, IndrovÃ¡ M, BieblovÃ¡ J, Å ÃmovÃ¡ J, TruxovÃ¡ I, et al. Dendritic cells pulsed with tumor cells killed by high hydrostatic pressure induce strong immune responses and display therapeutic effects both in murine TC-1 and TRAMP-C2 tumors when combined with docetaxel chemotherapy. Int J Oncol. 2016;48:953-64 pubmed publisher
	Martínez Torres A, Reyes Ruiz A, Calvillo Rodriguez K, Alvarez Valadez K, Uscanga Palomeque A, Tamez Guerra R, et al. IMMUNEPOTENT CRP induces DAMPS release and ROS-dependent autophagosome formation in HeLa and MCF-7 cells. BMC Cancer. 2020;20:647 pubmed publisher
	Arshad N, Cresswell P. Tumor-associated calreticulin variants functionally compromise the peptide loading complex and impair its recruitment of MHC-I. J Biol Chem. 2018;293:9555-9569 pubmed publisher
	D Eliseo D, Di Renzo L, Santoni A, Velotti F. Docosahexaenoic acid (DHA) promotes immunogenic apoptosis in human multiple myeloma cells, induces autophagy and inhibits STAT3 in both tumor and dendritic cells. Genes Cancer. 2017;8:426-437 pubmed publisher
	Epple L, Dodd R, Merz A, Dechkovskaia A, Herring M, Winston B, et al. Induction of the unfolded protein response drives enhanced metabolism and chemoresistance in glioma cells. PLoS ONE. 2013;8:e73267 pubmed publisher
	Kavaliauskiene S, Nymark C, Bergan J, Simm R, Sylvänne T, Simolin H, et al. Cell density-induced changes in lipid composition and intracellular trafficking. Cell Mol Life Sci. 2014;71:1097-116 pubmed publisher
	Gabillet J, Millet A, Pederzoli Ribeil M, Tacnet Delorme P, Guillevin L, Mouthon L, et al. Proteinase 3, the autoantigen in granulomatosis with polyangiitis, associates with calreticulin on apoptotic neutrophils, impairs macrophage phagocytosis, and promotes inflammation. J Immunol. 2012;189:2574-83 pubmed publisher
	Mu T, Ong D, Wang Y, Balch W, Yates J, Segatori L, et al. Chemical and biological approaches synergize to ameliorate protein-folding diseases. Cell. 2008;134:769-81 pubmed publisher
	Pagliuca M, Lerose R, Cigliano S, Leone A. Regulation by heavy metals and temperature of the human BAG-3 gene, a modulator of Hsp70 activity. FEBS Lett. 2003;541:11-5 pubmed
	Broquet A, Thomas G, Masliah J, Trugnan G, Bachelet M. Expression of the molecular chaperone Hsp70 in detergent-resistant microdomains correlates with its membrane delivery and release. J Biol Chem. 2003;278:21601-6 pubmed
	Partridge C, Beech D, Sivaprasadarao A. Identification and pharmacological correction of a membrane trafficking defect associated with a mutation in the sulfonylurea receptor causing familial hyperinsulinism. J Biol Chem. 2001;276:35947-52 pubmed
	Morello J, Salahpour A, Laperriere A, Bernier V, Arthus M, Lonergan M, et al. Pharmacological chaperones rescue cell-surface expression and function of misfolded V2 vasopressin receptor mutants. J Clin Invest. 2000;105:887-95 pubmed

product information

Product ID :

ADI-SPA-601

Catalog Number :

ADI-SPA-601-D

Product Name :

Calreticulin monoclonal antibody (FMC 75)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

24039668, 23921715, 22844112, 18775310, 12706811, 12682040, 11457841, 10749568

Clone :

FMC 75

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Monkey

Background :

Calreticulin (CRT) is a multifunctional, multi-compartmental protein most abundant in the ER lumen. CRT contains the ER-retrieval sequence, KDEL, and has been best characterized as a soluble molecular chaperone of new or misfolded proteins and a Ca2+- binding protein. Both CRT and its membrane bound homolog, Calnexin (CNX) interact with proteins and glycoproteins that have monoglucosylated N-glycans. The CRT/CNX cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligimerization, regulates ER degradation, and provides quality control by preventing incompletely folded glycoproteins from exiting to the Golgi complex.

ALTnames :

CALR, CRT

Immunogen :

Recombinant calreticulin.

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Predicted MW of ~63kDa.

Application Summary :

ICC, IHC (PS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage