This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
[pSer15]HSP27 polyclonal antibody
catalog :
ADI-SPA-525-D
quantity :
50 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, dogs, bovine
application :
western blot, immunohistochemistry, immunocytochemistry
product information
Product ID :
ADI-SPA-525
Catalog Number :
ADI-SPA-525-D
Product Name :
[pSer15]HSP27 polyclonal antibody
Host species :
Rabbit
Clonality :
Polyclonal
Antigen modification :
Phosphorylated
Product Type :
Antibody
Size :
50 µg
SPECIES REACTIVITY :
Human, Monkey, Guinea pig, Dog, Bovine
Background :
Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.
ALTnames :
HspB1, Heat shock protein 27
Immunogen :
Synthetic peptide corresponding to a portion of human HSP27 phosphorylated at Ser15.
Buffer :
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~27kDa by Western blot.
Application Summary :
IF, IHC, WB
Storage :
-20°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.