product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
HSP47 monoclonal antibody (M16.10A1)
catalog :
ADI-SPA-470-F
quantity :
200 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M16.10A1

The same clone is also sold as:
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
more info or order :
Enzo Life Sciences product webpage
citations: 21
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; loading ...; fig 5a
  • western blot; mouse; loading ...; fig 6c
Ishikawa Y, Ito S, Nagata K, Sakai L, Bachinger H. Intracellular mechanisms of molecular recognition and sorting for transport of large extracellular matrix molecules. Proc Natl Acad Sci U S A. 2016;113:E6036-E6044 pubmed
  • immunohistochemistry - frozen section; rat; 1:1000; fig 1
Oka T, Ohta K, Kanazawa T, Nakamura K. Interaction between Macrophages and Fibroblasts during Wound Healing of Burn Injuries in Rats. Kurume Med J. 2016;62:59-66 pubmed publisher
  • immunohistochemistry; human; fig 1
del Rey M, Faré R, Usategui A, Cañete J, Bravo B, Galindo M, et al. CD271(+) stromal cells expand in arthritic synovium and exhibit a proinflammatory phenotype. Arthritis Res Ther. 2016;18:66 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 1
Jeanne M, Jorgensen J, Gould D. Molecular and Genetic Analyses of Collagen Type IV Mutant Mouse Models of Spontaneous Intracerebral Hemorrhage Identify Mechanisms for Stroke Prevention. Circulation. 2015;131:1555-65 pubmed publisher
  • immunoprecipitation; human; 1:500
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:500
Durán I, Nevarez L, Sarukhanov A, Wu S, Lee K, Krejci P, et al. HSP47 and FKBP65 cooperate in the synthesis of type I procollagen. Hum Mol Genet. 2015;24:1918-28 pubmed publisher
McCaughey J, Stevenson N, Cross S, Stephens D. ER-to-Golgi trafficking of procollagen in the absence of large carriers. J Cell Biol. 2019;218:929-948 pubmed publisher
Hsu C, Hsu C, Hsueh C, Wang C, Wu Y, Wu C, et al. Identification and Characterization of Potential Biomarkers by Quantitative Tissue Proteomics of Primary Lung Adenocarcinoma. Mol Cell Proteomics. 2016;15:2396-410 pubmed publisher
Ito S, Nagata K. Mutants of collagen-specific molecular chaperone Hsp47 causing osteogenesis imperfecta are structurally unstable with weak binding affinity to collagen. Biochem Biophys Res Commun. 2016;469:437-42 pubmed publisher
Lindert U, Weis M, Rai J, Seeliger F, Hausser I, Leeb T, et al. Molecular Consequences of the SERPINH1/HSP47 Mutation in the Dachshund Natural Model of Osteogenesis Imperfecta. J Biol Chem. 2015;290:17679-89 pubmed publisher
Kitamura M, Nishino T, Obata Y, Oka S, Abe S, Muta K, et al. The kampo medicine Daikenchuto inhibits peritoneal fibrosis in mice. Biol Pharm Bull. 2015;38:193-200 pubmed publisher
Taguchi Y, Imaoka K, Kataoka M, Uda A, Nakatsu D, Horii Okazaki S, et al. Yip1A, a novel host factor for the activation of the IRE1 pathway of the unfolded protein response during Brucella infection. PLoS Pathog. 2015;11:e1004747 pubmed publisher
Lee R, Reese C, Bonner M, Tourkina E, Hajdu Z, Riemer E, et al. Bleomycin delivery by osmotic minipump: similarity to human scleroderma interstitial lung disease. Am J Physiol Lung Cell Mol Physiol. 2014;306:L736-48 pubmed publisher
Xu C, Mikami T, Nakamura T, Tsuruta T, Nakada N, Yanagisawa N, et al. Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: the roles of Hsp47 and basic fibroblast growth factor. Pathol Res Pract. 2013;209:69-74 pubmed publisher
Valsecchi F, Grefte S, Roestenberg P, Joosten Wagenaars J, Smeitink J, Willems P, et al. Primary fibroblasts of NDUFS4(-/-) mice display increased ROS levels and aberrant mitochondrial morphology. Mitochondrion. 2013;13:436-43 pubmed publisher
Kurashige T, Abe K, Furusu A, Miyazaki M, Obata Y, Xia Z, et al. Renoprotective effect of azelnidipine in rats. Biol Pharm Bull. 2008;31:2237-44 pubmed
Smith M, Cake M, Ghosh P, Schiavinato A, Read R, Little C. Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy. Rheumatology (Oxford). 2008;47:1172-8 pubmed publisher
Li D, Guang W, Abuzeid W, Roy S, Gao G, Sauk J, et al. Novel adenoviral gene delivery system targeted against head and neck cancer. Laryngoscope. 2008;118:650-8 pubmed publisher
Gould D, Marchant J, Savinova O, Smith R, John S. Col4a1 mutation causes endoplasmic reticulum stress and genetically modifiable ocular dysgenesis. Hum Mol Genet. 2007;16:798-807 pubmed
Koide T, Nishikawa Y, Asada S, Yamazaki C, Takahara Y, Homma D, et al. Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins. J Biol Chem. 2006;281:11177-85 pubmed
Koide T, Asada S, Takahara Y, Nishikawa Y, Nagata K, Kitagawa K. Specific recognition of the collagen triple helix by chaperone HSP47: minimal structural requirement and spatial molecular orientation. J Biol Chem. 2006;281:3432-8 pubmed
Delany J, Floyd Z, Zvonic S, Smith A, Gravois A, Reiners E, et al. Proteomic analysis of primary cultures of human adipose-derived stem cells: modulation by Adipogenesis. Mol Cell Proteomics. 2005;4:731-40 pubmed
product information
Product ID :
ADI-SPA-470
Catalog Number :
ADI-SPA-470-F
Product Name :
HSP47 monoclonal antibody (M16.10A1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
200 µg
list of Pubmed id :
26692483, 26004778, 25747978, 25742138, 24583879, 23265436, 23234723, 19043206, 18565987, 18176343
Clone :
M16.10A1
Isotype :
IgG2b
SPECIES REACTIVITY :
Human, Mouse, Rat, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Dog, Chicken, Bovine
Background :
Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.
ALTnames :
SerpinH1, Colligin, Gp46, Heat shock protein 47, Collagen-binding protein
Immunogen :
Native rat HSP47.
Buffer :
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Uses :
Detects a band of ~47kDa by Western blot.
Application Summary :
IHC, WB
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.