HSP47 monoclonal antibody (M16.10A1) | Enzo Life Sciences ADI-SPA-470-D product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSP47 monoclonal antibody (M16.10A1)

catalog :

ADI-SPA-470-D

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M16.10A1

The same clone is also sold as:

Novus Biologicals: NBP1-97491-0.05mg
Abnova: MAB16132

reactivity :

human, mouse, rat, dogs, chicken, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation, immunocytochemistry knockout validation

more info or order :

Enzo Life Sciences product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; mouse; loading ...; fig 5a western blot knockout validation; mouse; loading ...; fig 6c	Ishikawa Y, Ito S, Nagata K, Sakai L, Bachinger H. Intracellular mechanisms of molecular recognition and sorting for transport of large extracellular matrix molecules. Proc Natl Acad Sci U S A. 2016;113:E6036-E6044 pubmed
immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 4g immunocytochemistry; human; 1:1000; loading ...; fig 4e	Hemanthakumar K, Fang S, Anisimov A, Mäyränpää M, Mervaala E, Kivela R. Cardiovascular disease risk factors induce mesenchymal features and senescence in mouse cardiac endothelial cells. elife. 2021;10: pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000; fig 1	Oka T, Ohta K, Kanazawa T, Nakamura K. Interaction between Macrophages and Fibroblasts during Wound Healing of Burn Injuries in Rats. Kurume Med J. 2016;62:59-66 pubmed publisher
western blot; human; fig s2	Hsu C, Hsu C, Hsueh C, Wang C, Wu Y, Wu C, et al. Identification and Characterization of Potential Biomarkers by Quantitative Tissue Proteomics of Primary Lung Adenocarcinoma. Mol Cell Proteomics. 2016;15:2396-410 pubmed publisher
immunohistochemistry; human; fig 1	del Rey M, FarÃ© R, Usategui A, CaÃ±ete J, Bravo B, Galindo M, et al. CD271(+) stromal cells expand in arthritic synovium and exhibit a proinflammatory phenotype. Arthritis Res Ther. 2016;18:66 pubmed publisher
immunocytochemistry; dogs; 1:100 western blot; dogs; 1:1000; fig 1	Lindert U, Weis M, Rai J, Seeliger F, Hausser I, Leeb T, et al. Molecular Consequences of the SERPINH1/HSP47 Mutation in the Dachshund Natural Model of Osteogenesis Imperfecta. J Biol Chem. 2015;290:17679-89 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; fig 1	Jeanne M, Jorgensen J, Gould D. Molecular and Genetic Analyses of Collagen Type IV Mutant Mouse Models of Spontaneous Intracerebral Hemorrhage Identify Mechanisms for Stroke Prevention. Circulation. 2015;131:1555-65 pubmed publisher
immunoprecipitation; human; 1:500 immunocytochemistry; human; 1:100 western blot; human; 1:500	Durán I, Nevarez L, Sarukhanov A, Wu S, Lee K, Krejci P, et al. HSP47 and FKBP65 cooperate in the synthesis of type I procollagen. Hum Mol Genet. 2015;24:1918-28 pubmed publisher
	Francisco J, Zhang Y, Nakada Y, Jeong J, Huang C, Ivessa A, et al. AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis. Sci Rep. 2021;11:10553 pubmed publisher
	McCaughey J, Stevenson N, Cross S, Stephens D. ER-to-Golgi trafficking of procollagen in the absence of large carriers. J Cell Biol. 2019;218:929-948 pubmed publisher
	Ito S, Nagata K. Mutants of collagen-specific molecular chaperone Hsp47 causing osteogenesis imperfecta are structurally unstable with weak binding affinity to collagen. Biochem Biophys Res Commun. 2016;469:437-42 pubmed publisher
	Kitamura M, Nishino T, Obata Y, Oka S, Abe S, Muta K, et al. The kampo medicine Daikenchuto inhibits peritoneal fibrosis in mice. Biol Pharm Bull. 2015;38:193-200 pubmed publisher
	Taguchi Y, Imaoka K, Kataoka M, Uda A, Nakatsu D, Horii Okazaki S, et al. Yip1A, a novel host factor for the activation of the IRE1 pathway of the unfolded protein response during Brucella infection. PLoS Pathog. 2015;11:e1004747 pubmed publisher
	Lee R, Reese C, Bonner M, Tourkina E, Hajdu Z, Riemer E, et al. Bleomycin delivery by osmotic minipump: similarity to human scleroderma interstitial lung disease. Am J Physiol Lung Cell Mol Physiol. 2014;306:L736-48 pubmed publisher
	Xu C, Mikami T, Nakamura T, Tsuruta T, Nakada N, Yanagisawa N, et al. Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: the roles of Hsp47 and basic fibroblast growth factor. Pathol Res Pract. 2013;209:69-74 pubmed publisher
	Valsecchi F, Grefte S, Roestenberg P, Joosten Wagenaars J, Smeitink J, Willems P, et al. Primary fibroblasts of NDUFS4(-/-) mice display increased ROS levels and aberrant mitochondrial morphology. Mitochondrion. 2013;13:436-43 pubmed publisher
	Kurashige T, Abe K, Furusu A, Miyazaki M, Obata Y, Xia Z, et al. Renoprotective effect of azelnidipine in rats. Biol Pharm Bull. 2008;31:2237-44 pubmed
	Smith M, Cake M, Ghosh P, Schiavinato A, Read R, Little C. Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy. Rheumatology (Oxford). 2008;47:1172-8 pubmed publisher
	Li D, Guang W, Abuzeid W, Roy S, Gao G, Sauk J, et al. Novel adenoviral gene delivery system targeted against head and neck cancer. Laryngoscope. 2008;118:650-8 pubmed publisher
	Gould D, Marchant J, Savinova O, Smith R, John S. Col4a1 mutation causes endoplasmic reticulum stress and genetically modifiable ocular dysgenesis. Hum Mol Genet. 2007;16:798-807 pubmed
	Koide T, Nishikawa Y, Asada S, Yamazaki C, Takahara Y, Homma D, et al. Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins. J Biol Chem. 2006;281:11177-85 pubmed
	Koide T, Asada S, Takahara Y, Nishikawa Y, Nagata K, Kitagawa K. Specific recognition of the collagen triple helix by chaperone HSP47: minimal structural requirement and spatial molecular orientation. J Biol Chem. 2006;281:3432-8 pubmed
	Delany J, Floyd Z, Zvonic S, Smith A, Gravois A, Reiners E, et al. Proteomic analysis of primary cultures of human adipose-derived stem cells: modulation by Adipogenesis. Mol Cell Proteomics. 2005;4:731-40 pubmed

product information

Product ID :

ADI-SPA-470

Catalog Number :

ADI-SPA-470-D

Product Name :

HSP47 monoclonal antibody (M16.10A1)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

26692483, 26004778, 25747978, 25742138, 24583879, 23265436, 23234723, 19043206, 18565987, 18176343

Clone :

M16.10A1

Isotype :

IgG2b

SPECIES REACTIVITY :

Human, Mouse, Rat, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Dog, Chicken, Bovine

Background :

Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.

ALTnames :

SerpinH1, Colligin, Gp46, Heat shock protein 47, Collagen-binding protein

Immunogen :

Native rat HSP47.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~47kDa by Western blot.

Application Summary :

IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage