HSP40/Hdj2 polyclonal antibody | Enzo Life Sciences ADI-SPA-405-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HSP40/Hdj2 polyclonal antibody

catalog :

ADI-SPA-405-050

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

citations: 1

Reference
Veit G, Oliver K, Apaja P, Perdomo D, Bidaud Meynard A, Lin S, et al. Ribosomal Stalk Protein Silencing Partially Corrects the ΔF508-CFTR Functional Expression Defect. PLoS Biol. 2016;14:e1002462 pubmed publisher

product information

Product ID :

ADI-SPA-405

Catalog Number :

ADI-SPA-405-050

Product Name :

HSP40/Hdj2 polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

22777893, 20885985

SPECIES REACTIVITY :

Human, Mouse, Rat, Hamster

Background :

Hdj2 is an approximately 40kDa member of the heat shock protein (Hsp40) family, a eukaryotic homolog of the E. coli DnaJ protein. Eukaryotic DnaJ-like proteins share structural domain conservation with DnaJ namely: 1) an N-terminal ~70 amino acid J domain, 2) a glycine- and phenylalanine-rich domain, 3) a cysteine-rich zinc finger domain, and 4) a poorly conserved C-terminal domain. The human constitutive Hdj2 protein shares full domain conservation with DnaJ, as opposed to the human inducible Hdj1 which lacks the zinc-finger domain. The Hsp40 J domain regulates its interaction with Hsp70 (E. coli DnaK), increasing Hsp70 ATPase activity and in turn enhancing substrate binding by the Hsp70 chaperone. Hsp40 co-chaperones are involved in nearly all aspects of protein synthesis and secretion because of their importance in Hsp70 function, and they are also thought to have an intrinsic ability to bind and fold some misfolded proteins. Hdj2 has been specifically shown to interact with Hsc70, the constitutive form of Hsp70, in the biogenesis of the cystic fibrosis transmembrane conductance regulator (CFTR), mutations of which are known to cause inherited cystic fibrosis.

ALTnames :

Heat shock protein 40

Immunogen :

Recombinant human HSP40/Hdj2.

Specificity :

Has high specificity for the constitutively expressed Hdj2 compared to Hdj1 using recombinant controls.

Buffer :

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Uses :

Detects a band of ~45kDa by Western blot.

Application Summary :

Storage :

-20°C